{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-1416"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-1416","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Feeding Female Growing Rats Different Sources of Omega-3 Polyunsaturated Fatty Acids Affects Lung Fatty Acid Composition and Gene Expression","abstract":"Justification: The lungs are constantly exposed to oxidative stress and inflammation which increase the risk of lung damage. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), found in different dietary sources, regulate the expression of various genes involved in eicosanoid production and oxidative stability.;Objective: to determine whether providing different sources of n-3 PUFAs will affect the fatty acid composition, inflammation and oxidation in the lungs of growing rats.;Methods: Growing (age 28 d) Sprague-Dawley rats were randomly assigned (n=10/group) to diets consisting of either corn oil (CO) or n-3 PUFA rich flaxseed (FO), krill (KO), salmon (SO) or tuna (TO) oil for 8 weeks. Diet and tissue fatty acid composition was analyzed by gas chromatography. The enzymes involved in eicosanoid synthesis, 5-lipoxygenase (5-LOX) and cyclooxygenase II (COX II), and the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase 1 (SOD1), were determined using real-time polymerase chain reaction.;Results: Rats fed FO, with the highest alpha-linolenic acid (ALA, 18:2n-3) content, had the highest (P<0.001) lung ALA deposition. Rats fed KO, with the highest eicosapentaenoic acid (EPA, 20:5n3) content, had the highest (P<0.001) lung EPA deposition compared to all diet groups, except SO-fed rats. Rats fed TO, with the highest docosahexaenoic acid (DHA, 22:6n-3) content, had the highest (P<0.001) lung DHA deposition compared to rats fed CO or FO. The relative expression of 5-LOX was significantly up-regulated (P=0.007) in rats fed FO compared to CO-fed rats. Expression of the antioxidant defense enzyme, SOD1 was down-regulated (P=0.01) in TO-fed rats compared to rats fed CO. Dietary TO has an EPA/DHA ratio of 1:2 and had a higher ( P<0.001) lung DHA content compared to rats fed CO.;Significance: The fatty acid composition of the lungs reflected dietary intake. The increase in o-3 PUFAs in lung tissue may affect the risk of lung damage, and in turn, susceptibility to lung diseases by altering gene expression of inflammation and oxidative stability.","abstract_html":"Justification: The lungs are constantly exposed to oxidative stress and inflammation which increase the risk of lung damage. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), found in different dietary sources, regulate the expression of various genes involved in eicosanoid production and oxidative stability.;Objective: to determine whether providing different sources of n-3 PUFAs will affect the fatty acid composition, inflammation and oxidation in the lungs of growing rats.;Methods: Growing (age 28 d) Sprague-Dawley rats were randomly assigned (n=10/group) to diets consisting of either corn oil (CO) or n-3 PUFA rich flaxseed (FO), krill (KO), salmon (SO) or tuna (TO) oil for 8 weeks. Diet and tissue fatty acid composition was analyzed by gas chromatography. The enzymes involved in eicosanoid synthesis, 5-lipoxygenase (5-LOX) and cyclooxygenase II (COX II), and the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase 1 (SOD1), were determined using real-time polymerase chain reaction.;Results: Rats fed FO, with the highest alpha-linolenic acid (ALA, 18:2n-3) content, had the highest (P&lt;0.001) lung ALA deposition. Rats fed KO, with the highest eicosapentaenoic acid (EPA, 20:5n3) content, had the highest (P&lt;0.001) lung EPA deposition compared to all diet groups, except SO-fed rats. Rats fed TO, with the highest docosahexaenoic acid (DHA, 22:6n-3) content, had the highest (P&lt;0.001) lung DHA deposition compared to rats fed CO or FO. The relative expression of 5-LOX was significantly up-regulated (P=0.007) in rats fed FO compared to CO-fed rats. Expression of the antioxidant defense enzyme, SOD1 was down-regulated (P=0.01) in TO-fed rats compared to rats fed CO. Dietary TO has an EPA/DHA ratio of 1:2 and had a higher ( P&lt;0.001) lung DHA content compared to rats fed CO.;Significance: The fatty acid composition of the lungs reflected dietary intake. The increase in o-3 PUFAs in lung tissue may affect the risk of lung damage, and in turn, susceptibility to lung diseases by altering gene expression of inflammation and oxidative stability.","abstract_has_math":false,"creators":["Dalzot, Maria L."],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Animal and Nutritional Sciences","degree_department":null,"school":null,"contributors":["Janet C. Tou","Vagner Benedito","Joseph Moritz"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-05-01T07:00:00Z","date_published":"2012-05-01T07:00:00Z","updated_at":"2026-07-24T06:14:24Z","subjects":["Nutrition"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/413"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/413","href":"https://researchrepository.wvu.edu/etd/413","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.413","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Janet C. Tou","Vagner Benedito","Joseph Moritz"]},{"key":"dc:creator","label":"Author","values":["Dalzot, Maria L."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal and Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nutrition"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.413","https://researchrepository.wvu.edu/etd/413"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Justification: The lungs are constantly exposed to oxidative stress and inflammation which increase the risk of lung damage. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), found in different dietary sources, regulate the expression of various genes involved in eicosanoid production and oxidative stability.;Objective: to determine whether providing different sources of n-3 PUFAs will affect the fatty acid composition, inflammation and oxidation in the lungs of growing rats.;Methods: Growing (age 28 d) Sprague-Dawley rats were randomly assigned (n=10/group) to diets consisting of either corn oil (CO) or n-3 PUFA rich flaxseed (FO), krill (KO), salmon (SO) or tuna (TO) oil for 8 weeks. Diet and tissue fatty acid composition was analyzed by gas chromatography. The enzymes involved in eicosanoid synthesis, 5-lipoxygenase (5-LOX) and cyclooxygenase II (COX II), and the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase 1 (SOD1), were determined using real-time polymerase chain reaction.;Results: Rats fed FO, with the highest alpha-linolenic acid (ALA, 18:2n-3) content, had the highest (P<0.001) lung ALA deposition. Rats fed KO, with the highest eicosapentaenoic acid (EPA, 20:5n3) content, had the highest (P<0.001) lung EPA deposition compared to all diet groups, except SO-fed rats. Rats fed TO, with the highest docosahexaenoic acid (DHA, 22:6n-3) content, had the highest (P<0.001) lung DHA deposition compared to rats fed CO or FO. The relative expression of 5-LOX was significantly up-regulated (P=0.007) in rats fed FO compared to CO-fed rats. Expression of the antioxidant defense enzyme, SOD1 was down-regulated (P=0.01) in TO-fed rats compared to rats fed CO. Dietary TO has an EPA/DHA ratio of 1:2 and had a higher ( P<0.001) lung DHA content compared to rats fed CO.;Significance: The fatty acid composition of the lungs reflected dietary intake. The increase in o-3 PUFAs in lung tissue may affect the risk of lung damage, and in turn, susceptibility to lung diseases by altering gene expression of inflammation and oxidative stability."]},{"key":"dc:title","label":"Title","values":["Feeding Female Growing Rats Different Sources of Omega-3 Polyunsaturated Fatty Acids Affects Lung Fatty Acid Composition and Gene Expression"]}]}],"canonical_facts":{"dc:contributor":["Janet C. Tou","Vagner Benedito","Joseph Moritz"],"dc:creator":["Dalzot, Maria L."],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["Justification: The lungs are constantly exposed to oxidative stress and inflammation which increase the risk of lung damage. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), found in different dietary sources, regulate the expression of various genes involved in eicosanoid production and oxidative stability.;Objective: to determine whether providing different sources of n-3 PUFAs will affect the fatty acid composition, inflammation and oxidation in the lungs of growing rats.;Methods: Growing (age 28 d) Sprague-Dawley rats were randomly assigned (n=10/group) to diets consisting of either corn oil (CO) or n-3 PUFA rich flaxseed (FO), krill (KO), salmon (SO) or tuna (TO) oil for 8 weeks. Diet and tissue fatty acid composition was analyzed by gas chromatography. The enzymes involved in eicosanoid synthesis, 5-lipoxygenase (5-LOX) and cyclooxygenase II (COX II), and the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase 1 (SOD1), were determined using real-time polymerase chain reaction.;Results: Rats fed FO, with the highest alpha-linolenic acid (ALA, 18:2n-3) content, had the highest (P<0.001) lung ALA deposition. Rats fed KO, with the highest eicosapentaenoic acid (EPA, 20:5n3) content, had the highest (P<0.001) lung EPA deposition compared to all diet groups, except SO-fed rats. Rats fed TO, with the highest docosahexaenoic acid (DHA, 22:6n-3) content, had the highest (P<0.001) lung DHA deposition compared to rats fed CO or FO. The relative expression of 5-LOX was significantly up-regulated (P=0.007) in rats fed FO compared to CO-fed rats. Expression of the antioxidant defense enzyme, SOD1 was down-regulated (P=0.01) in TO-fed rats compared to rats fed CO. Dietary TO has an EPA/DHA ratio of 1:2 and had a higher ( P<0.001) lung DHA content compared to rats fed CO.;Significance: The fatty acid composition of the lungs reflected dietary intake. The increase in o-3 PUFAs in lung tissue may affect the risk of lung damage, and in turn, susceptibility to lung diseases by altering gene expression of inflammation and oxidative stability."],"dc:identifier":["https://doi.org/10.33915/etd.413","https://researchrepository.wvu.edu/etd/413"],"dc:subject":["Nutrition"],"dc:title":["Feeding Female Growing Rats Different Sources of Omega-3 Polyunsaturated Fatty Acids Affects Lung Fatty Acid Composition and Gene Expression"],"thesis:degree_discipline":["Animal and Nutritional Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:14:24Z"}