{"id":{"repo_id":"wvu","oai_identifier":"oai:researchrepository.wvu.edu:etd-1100"},"canonical_url":"https://search.dev.ndltd.org/etd/wvu/oai:researchrepository.wvu.edu:etd-1100","repository":{"repo_id":"wvu","name":"West Virginia University","base_url":"https://researchrepository.wvu.edu/do/oai/"},"display":{"title":"Evaluation of non-invasive biomarkers for carcinogenic exposure to cigarette smoke","abstract":"Purpose. To investigate urinary 1-hydroxypyrene glucuronide (1-OHPG) and benzo (a) pyrene 7,10/8,-tetrol (BaPT) levels as non-invasive biomarkers for exposure to cigarette smoke.;Methods. Volunteers, smokers ({dollar}\\ge{dollar}20 cigarettes/day) and non-smokers (5 females + 6 males in each group), provided multiple urine samples. Urine samples from psoriasis patients undergoing coal tar therapy were also obtained. Urinary BaPT and 1-OHPG levels were assayed.;Results. While BaPT levels ranged from undetectable to 11 pmol/10ml in psoriasis patients (mean = 4 {dollar}\\pm{dollar} 3.4 pmol/10ml), most samples from smokers or non-smokers had undetectable levels (limit of detection = 25 fmol/10ml urine). Urinary 1-OHPG levels were significantly higher among smokers (mean log 1-OHPG = 1.72 {dollar}\\pm{dollar} 0.93) than among nonsmokers (mean log 1-OHPG = 0.80 {dollar}\\pm{dollar} 0.92) (P {dollar}<{dollar} 0.05). Conclusion. Higher assay sensitivity is required to monitor urinary levels of BaPT in smokers. 1-OHPG levels in urine may prove useful in monitoring exposure to carcinogenic PAH from tobacco smoke.","abstract_html":"Purpose. To investigate urinary 1-hydroxypyrene glucuronide (1-OHPG) and benzo (a) pyrene 7,10/8,-tetrol (BaPT) levels as non-invasive biomarkers for exposure to cigarette smoke.;Methods. Volunteers, smokers ({dollar}\\ge{dollar}20 cigarettes/day) and non-smokers (5 females + 6 males in each group), provided multiple urine samples. Urine samples from psoriasis patients undergoing coal tar therapy were also obtained. Urinary BaPT and 1-OHPG levels were assayed.;Results. While BaPT levels ranged from undetectable to 11 pmol/10ml in psoriasis patients (mean = 4 {dollar}\\pm{dollar} 3.4 pmol/10ml), most samples from smokers or non-smokers had undetectable levels (limit of detection = 25 fmol/10ml urine). Urinary 1-OHPG levels were significantly higher among smokers (mean log 1-OHPG = 1.72 {dollar}\\pm{dollar} 0.93) than among nonsmokers (mean log 1-OHPG = 0.80 {dollar}\\pm{dollar} 0.92) (P {dollar}&lt;{dollar} 0.05). Conclusion. Higher assay sensitivity is required to monitor urinary levels of BaPT in smokers. 1-OHPG levels in urine may prove useful in monitoring exposure to carcinogenic PAH from tobacco smoke.","abstract_has_math":false,"creators":["Gudi, Girish Srinivas"],"institution":null,"degree_name":"MS","degree_level":"Thesis","degree_discipline":"Pharmaceutical Sciences","degree_department":null,"school":null,"contributors":["Mohamadi Sarkar","Timothy Tracy","Ainsley Weston","Ann Berry"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999-05-01T07:00:00Z","date_published":"1999-05-01T07:00:00Z","updated_at":"2026-07-24T06:14:02Z","subjects":["Pharmaceutical sciences","Toxicology","Oncology","Biochemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://researchrepository.wvu.edu/etd/97"],"render_values":[{"text":"https://researchrepository.wvu.edu/etd/97","href":"https://researchrepository.wvu.edu/etd/97","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.33915/etd.97","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mohamadi Sarkar","Timothy Tracy","Ainsley Weston","Ann Berry"]},{"key":"dc:creator","label":"Author","values":["Gudi, Girish Srinivas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-01-17T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Pharmaceutical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["MS"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Pharmaceutical sciences","Toxicology","Oncology","Biochemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/10.33915/etd.97","https://researchrepository.wvu.edu/etd/97"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Purpose. To investigate urinary 1-hydroxypyrene glucuronide (1-OHPG) and benzo (a) pyrene 7,10/8,-tetrol (BaPT) levels as non-invasive biomarkers for exposure to cigarette smoke.;Methods. Volunteers, smokers ({dollar}\\ge{dollar}20 cigarettes/day) and non-smokers (5 females + 6 males in each group), provided multiple urine samples. Urine samples from psoriasis patients undergoing coal tar therapy were also obtained. Urinary BaPT and 1-OHPG levels were assayed.;Results. While BaPT levels ranged from undetectable to 11 pmol/10ml in psoriasis patients (mean = 4 {dollar}\\pm{dollar} 3.4 pmol/10ml), most samples from smokers or non-smokers had undetectable levels (limit of detection = 25 fmol/10ml urine). Urinary 1-OHPG levels were significantly higher among smokers (mean log 1-OHPG = 1.72 {dollar}\\pm{dollar} 0.93) than among nonsmokers (mean log 1-OHPG = 0.80 {dollar}\\pm{dollar} 0.92) (P {dollar}<{dollar} 0.05). Conclusion. Higher assay sensitivity is required to monitor urinary levels of BaPT in smokers. 1-OHPG levels in urine may prove useful in monitoring exposure to carcinogenic PAH from tobacco smoke."]},{"key":"dc:title","label":"Title","values":["Evaluation of non-invasive biomarkers for carcinogenic exposure to cigarette smoke"]}]}],"canonical_facts":{"dc:contributor":["Mohamadi Sarkar","Timothy Tracy","Ainsley Weston","Ann Berry"],"dc:creator":["Gudi, Girish Srinivas"],"dc:date.available":["2019-01-17T08:00:00Z"],"dc:description.abstract":["Purpose. To investigate urinary 1-hydroxypyrene glucuronide (1-OHPG) and benzo (a) pyrene 7,10/8,-tetrol (BaPT) levels as non-invasive biomarkers for exposure to cigarette smoke.;Methods. Volunteers, smokers ({dollar}\\ge{dollar}20 cigarettes/day) and non-smokers (5 females + 6 males in each group), provided multiple urine samples. Urine samples from psoriasis patients undergoing coal tar therapy were also obtained. Urinary BaPT and 1-OHPG levels were assayed.;Results. While BaPT levels ranged from undetectable to 11 pmol/10ml in psoriasis patients (mean = 4 {dollar}\\pm{dollar} 3.4 pmol/10ml), most samples from smokers or non-smokers had undetectable levels (limit of detection = 25 fmol/10ml urine). Urinary 1-OHPG levels were significantly higher among smokers (mean log 1-OHPG = 1.72 {dollar}\\pm{dollar} 0.93) than among nonsmokers (mean log 1-OHPG = 0.80 {dollar}\\pm{dollar} 0.92) (P {dollar}<{dollar} 0.05). Conclusion. Higher assay sensitivity is required to monitor urinary levels of BaPT in smokers. 1-OHPG levels in urine may prove useful in monitoring exposure to carcinogenic PAH from tobacco smoke."],"dc:identifier":["https://doi.org/10.33915/etd.97","https://researchrepository.wvu.edu/etd/97"],"dc:subject":["Pharmaceutical sciences","Toxicology","Oncology","Biochemistry"],"dc:title":["Evaluation of non-invasive biomarkers for carcinogenic exposure to cigarette smoke"],"thesis:degree_discipline":["Pharmaceutical Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["MS"]},"updated_at":"2026-07-24T06:14:02Z"}