Contemporary and traditional methods of perifusional analysis of cells, pancreatic β cells in particular, involve the utilization of costly equipment exhibiting difficulties in setup and inflexibilities in operation and application. Microfluidic devices in general are inexpensive, easy to fabricate and are capable of screening multiple microenvironmental conditions, with the potential to generate concentration gradients for the study of biological phenomena such as cell migration. Utilization of these devices for cellular fluorescence imaging is also possible, paving the way for more sophisticated methods of dynamic assessment. Herein, we fabricated a pre-designed microfluidic device from photolithography, containing microwells capable of immobilizing human β islet or stem cell-derived β cells for perifusional analysis with low (2mM) and high (20mM) concentrations of glucose, following subsequent dynamic estimation of insulin produced using the insulin ELISA. The main aim was to compare and contrast insulin secretory behavior of human islet and stem cell-derived β cells with dynamic study of calcium ion activity during insulin exocytosis through fluorescence imaging, using an alternative method of assessment, namely, microfluidics.
","abstract_html":"<p>Contemporary and traditional methods of perifusional analysis of cells, pancreatic β cells in particular, involve the utilization of costly equipment exhibiting difficulties in setup and inflexibilities in operation and application. Microfluidic devices in general are inexpensive, easy to fabricate and are capable of screening multiple microenvironmental conditions, with the potential to generate concentration gradients for the study of biological phenomena such as cell migration. Utilization of these devices for cellular fluorescence imaging is also possible, paving the way for more sophisticated methods of dynamic assessment. Herein, we fabricated a pre-designed microfluidic device from photolithography, containing microwells capable of immobilizing human β islet or stem cell-derived β cells for perifusional analysis with low (2mM) and high (20mM) concentrations of glucose, following subsequent dynamic estimation of insulin produced using the insulin ELISA. The main aim was to compare and contrast insulin secretory behavior of human islet and stem cell-derived β cells with dynamic study of calcium ion activity during insulin exocytosis through fluorescence imaging, using an alternative method of assessment, namely, microfluidics.</p>","abstract_has_math":false,"creators":["Srivatsava, Arvind Rajamani"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Biomedical Engineering","degree_department":null,"school":null,"contributors":["Jeffrey Millman","Dennis Barbour Jeffrey Millman Amit Pathak"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-05-17T07:00:00Z","date_published":"2019-05-17T07:00:00Z","updated_at":"2026-07-24T06:13:23Z","subjects":["Microfluidics","beta cells","insulin","calcium","fluorescence imaging","GSIS","Engineering"],"languages":["English (en)"],"rights":["I have not registered my thesis with the U.S. Copyright Office, and do not intend to."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://openscholarship.wustl.edu/eng_etds/439"],"render_values":[{"text":"https://openscholarship.wustl.edu/eng_etds/439","href":"https://openscholarship.wustl.edu/eng_etds/439","code":true}]}]},"links":{"outbound_url":"https://doi.org/7936/m19n-nx31","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jeffrey Millman","Dennis Barbour Jeffrey Millman Amit Pathak"]},{"key":"dc:creator","label":"Author","values":["Srivatsava, Arvind Rajamani"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-05-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Engineering","McKelvey School of Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microfluidics","beta cells","insulin","calcium","fluorescence imaging","GSIS","Engineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English (en)"]},{"key":"dc:rights","label":"Dc Rights","values":["I have not registered my thesis with the U.S. Copyright Office, and do not intend to."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://doi.org/7936/m19n-nx31","https://openscholarship.wustl.edu/eng_etds/439"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Permanent URL: https://doi.org/7936/m19n-nx31"]},{"key":"dc:description.abstract","label":"Abstract","values":["Contemporary and traditional methods of perifusional analysis of cells, pancreatic β cells in particular, involve the utilization of costly equipment exhibiting difficulties in setup and inflexibilities in operation and application. Microfluidic devices in general are inexpensive, easy to fabricate and are capable of screening multiple microenvironmental conditions, with the potential to generate concentration gradients for the study of biological phenomena such as cell migration. Utilization of these devices for cellular fluorescence imaging is also possible, paving the way for more sophisticated methods of dynamic assessment. Herein, we fabricated a pre-designed microfluidic device from photolithography, containing microwells capable of immobilizing human β islet or stem cell-derived β cells for perifusional analysis with low (2mM) and high (20mM) concentrations of glucose, following subsequent dynamic estimation of insulin produced using the insulin ELISA. The main aim was to compare and contrast insulin secretory behavior of human islet and stem cell-derived β cells with dynamic study of calcium ion activity during insulin exocytosis through fluorescence imaging, using an alternative method of assessment, namely, microfluidics.
"]},{"key":"dc:title","label":"Title","values":["Microfluidic Assessment of Stem Cell-Derived Pancreatic Beta Cells"]}]}],"canonical_facts":{"dc:contributor":["Jeffrey Millman","Dennis Barbour Jeffrey Millman Amit Pathak"],"dc:creator":["Srivatsava, Arvind Rajamani"],"dc:date.available":["2019-05-09T07:00:00Z"],"dc:description":["Permanent URL: https://doi.org/7936/m19n-nx31"],"dc:description.abstract":["Contemporary and traditional methods of perifusional analysis of cells, pancreatic β cells in particular, involve the utilization of costly equipment exhibiting difficulties in setup and inflexibilities in operation and application. Microfluidic devices in general are inexpensive, easy to fabricate and are capable of screening multiple microenvironmental conditions, with the potential to generate concentration gradients for the study of biological phenomena such as cell migration. Utilization of these devices for cellular fluorescence imaging is also possible, paving the way for more sophisticated methods of dynamic assessment. Herein, we fabricated a pre-designed microfluidic device from photolithography, containing microwells capable of immobilizing human β islet or stem cell-derived β cells for perifusional analysis with low (2mM) and high (20mM) concentrations of glucose, following subsequent dynamic estimation of insulin produced using the insulin ELISA. The main aim was to compare and contrast insulin secretory behavior of human islet and stem cell-derived β cells with dynamic study of calcium ion activity during insulin exocytosis through fluorescence imaging, using an alternative method of assessment, namely, microfluidics.
"],"dc:identifier":["https://doi.org/7936/m19n-nx31","https://openscholarship.wustl.edu/eng_etds/439"],"dc:language":["English (en)"],"dc:rights":["I have not registered my thesis with the U.S. Copyright Office, and do not intend to."],"dc:subject":["Microfluidics","beta cells","insulin","calcium","fluorescence imaging","GSIS","Engineering"],"dc:title":["Microfluidic Assessment of Stem Cell-Derived Pancreatic Beta Cells"],"thesis:degree_discipline":["Biomedical Engineering","McKelvey School of Engineering"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T06:13:23Z"}