{"id":{"repo_id":"wku-diss","oai_identifier":"oai:digitalcommons.wku.edu:theses-1750"},"canonical_url":"https://search.dev.ndltd.org/etd/wku-diss/oai:digitalcommons.wku.edu:theses-1750","repository":{"repo_id":"wku-diss","name":"Western Kentucky University","base_url":"https://digitalcommons.wku.edu/do/oai/"},"display":{"title":"Study of the baiG Gene in Bile Acid 7α-Dehydroxylating Bacteria","abstract":"Recently, intestinal bile acid 7α-dehydroxylating bacteria have been implicated in cholesterol gallstone disease. Eubacterium sp. strain V.P.I. 12708, a bile acid 7α-dehydroxylating bacteria, contains multiple bile acid inducible (bai) genes which are located on a large bai operon. Genes of this operon encode the enzymes responsible for bile acid 7α-dehydroxylation. The baiG gene encodes a bile acid transporter in Eubacterium sp. strain V.P.I. 12708. Utilizing the polymerase chain reaction I determined the presence or absence of baiG-like genes in six bile acid 7α-dehydroxylating strains: Clostridium sp. Strain TN-271, Eubacterium sp. strain 1-10, Clostridium bifermentans strain 1-55, Clostridium sordellii strain Y67, Clostridium sp. strain HD-17, and Clostridium sp. strain TO-931. Results showed amplification in two of the bacterial strains at the predicted DNA fragment size. Partial DNA sequence analysis of the amplified baiG-like gene fragments revealed 96-97% homology with the baiG gene of Eubacterium sp. strain V.P.I. 12708. These data suggest that baiG-like genes are present in Clostridium sp. strain TN-271 and Eubacterium sp. strain I-10.","abstract_html":"Recently, intestinal bile acid 7α-dehydroxylating bacteria have been implicated in cholesterol gallstone disease. Eubacterium sp. strain V.P.I. 12708, a bile acid 7α-dehydroxylating bacteria, contains multiple bile acid inducible (bai) genes which are located on a large bai operon. Genes of this operon encode the enzymes responsible for bile acid 7α-dehydroxylation. The baiG gene encodes a bile acid transporter in Eubacterium sp. strain V.P.I. 12708. Utilizing the polymerase chain reaction I determined the presence or absence of baiG-like genes in six bile acid 7α-dehydroxylating strains: Clostridium sp. Strain TN-271, Eubacterium sp. strain 1-10, Clostridium bifermentans strain 1-55, Clostridium sordellii strain Y67, Clostridium sp. strain HD-17, and Clostridium sp. strain TO-931. Results showed amplification in two of the bacterial strains at the predicted DNA fragment size. Partial DNA sequence analysis of the amplified baiG-like gene fragments revealed 96-97% homology with the baiG gene of Eubacterium sp. strain V.P.I. 12708. These data suggest that baiG-like genes are present in Clostridium sp. strain TN-271 and Eubacterium sp. strain I-10.","abstract_has_math":false,"creators":["David, Michael"],"institution":null,"degree_name":"Master of Science","degree_level":null,"degree_discipline":"Department of Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999-05-01T07:00:00Z","date_published":"1999-05-01T07:00:00Z","updated_at":"2026-07-24T06:07:48Z","subjects":["Medical Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.wku.edu/theses/747","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["David, Michael"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Department of Biology"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Medical Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.wku.edu/theses/747"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Recently, intestinal bile acid 7α-dehydroxylating bacteria have been implicated in cholesterol gallstone disease. Eubacterium sp. strain V.P.I. 12708, a bile acid 7α-dehydroxylating bacteria, contains multiple bile acid inducible (bai) genes which are located on a large bai operon. Genes of this operon encode the enzymes responsible for bile acid 7α-dehydroxylation. The baiG gene encodes a bile acid transporter in Eubacterium sp. strain V.P.I. 12708. Utilizing the polymerase chain reaction I determined the presence or absence of baiG-like genes in six bile acid 7α-dehydroxylating strains: Clostridium sp. Strain TN-271, Eubacterium sp. strain 1-10, Clostridium bifermentans strain 1-55, Clostridium sordellii strain Y67, Clostridium sp. strain HD-17, and Clostridium sp. strain TO-931. Results showed amplification in two of the bacterial strains at the predicted DNA fragment size. Partial DNA sequence analysis of the amplified baiG-like gene fragments revealed 96-97% homology with the baiG gene of Eubacterium sp. strain V.P.I. 12708. These data suggest that baiG-like genes are present in Clostridium sp. strain TN-271 and Eubacterium sp. strain I-10."]},{"key":"dc:title","label":"Title","values":["Study of the baiG Gene in Bile Acid 7α-Dehydroxylating Bacteria"]}]}],"canonical_facts":{"dc:creator":["David, Michael"],"dc:description.abstract":["Recently, intestinal bile acid 7α-dehydroxylating bacteria have been implicated in cholesterol gallstone disease. Eubacterium sp. strain V.P.I. 12708, a bile acid 7α-dehydroxylating bacteria, contains multiple bile acid inducible (bai) genes which are located on a large bai operon. Genes of this operon encode the enzymes responsible for bile acid 7α-dehydroxylation. The baiG gene encodes a bile acid transporter in Eubacterium sp. strain V.P.I. 12708. Utilizing the polymerase chain reaction I determined the presence or absence of baiG-like genes in six bile acid 7α-dehydroxylating strains: Clostridium sp. Strain TN-271, Eubacterium sp. strain 1-10, Clostridium bifermentans strain 1-55, Clostridium sordellii strain Y67, Clostridium sp. strain HD-17, and Clostridium sp. strain TO-931. Results showed amplification in two of the bacterial strains at the predicted DNA fragment size. Partial DNA sequence analysis of the amplified baiG-like gene fragments revealed 96-97% homology with the baiG gene of Eubacterium sp. strain V.P.I. 12708. These data suggest that baiG-like genes are present in Clostridium sp. strain TN-271 and Eubacterium sp. strain I-10."],"dc:identifier":["https://digitalcommons.wku.edu/theses/747"],"dc:subject":["Medical Sciences"],"dc:title":["Study of the baiG Gene in Bile Acid 7α-Dehydroxylating Bacteria"],"dc:type":["Thesis"],"thesis:degree_discipline":["Department of Biology"],"thesis:degree_name":["Master of Science"]},"updated_at":"2026-07-24T06:07:48Z"}