{"id":{"repo_id":"wfu","oai_identifier":"oai:wakespace.lib.wfu.edu:10339/90732"},"canonical_url":"https://search.dev.ndltd.org/etd/wfu/oai:wakespace.lib.wfu.edu:10339/90732","repository":{"repo_id":"wfu","name":"Wake Forest University","base_url":"https://wakespace.lib.wfu.edu/oai/request"},"display":{"title":"Dysregulated DJ1-Mediated Translation in Alzheimer's Disease","abstract":"Alzheimer’s Disease (AD) is a form of dementia with aberrant synaptic transmission. It has been hypothesized that disrupted calcium signaling may cause the digression from healthy memory to mild cognitive impairment (MCI) and eventually AD; and the search for the source of calcium dyshomeostasis continues still. The goal of this thesis is to take a bottom up approach to understanding the role of calcium in cellular memory during healthy and disease states. The mammalian target of rapamycin (mTOR) is a ubiquitously expressed kinase that governs protein synthesis, and is required for learning and memory. Here, we explore a downstream target of mTOR, the novel RNA-binding protein (RBP) Parkinson Protein 7 (DJ1) (Niere et al. 2016). Our data suggests, for the first time, that DJ1 is aberrantly expressed at the synaptic level in AD. Furthermore, a possible DJ1 target mRNA, CACNA2D2, is also aberrantly expressed at the synapses in AD, both at the mRNA and protein level. This work suggests that DJ1 may be a possible target upstream of L-type voltage-gated calcium channels (VGCC), and hence pose as a novel target for treatment of MCI, correcting calcium dynamics at the synapse.","abstract_html":"Alzheimer’s Disease (AD) is a form of dementia with aberrant synaptic transmission. It has been hypothesized that disrupted calcium signaling may cause the digression from healthy memory to mild cognitive impairment (MCI) and eventually AD; and the search for the source of calcium dyshomeostasis continues still. The goal of this thesis is to take a bottom up approach to understanding the role of calcium in cellular memory during healthy and disease states. The mammalian target of rapamycin (mTOR) is a ubiquitously expressed kinase that governs protein synthesis, and is required for learning and memory. Here, we explore a downstream target of mTOR, the novel RNA-binding protein (RBP) Parkinson Protein 7 (DJ1) (Niere et al. 2016). Our data suggests, for the first time, that DJ1 is aberrantly expressed at the synaptic level in AD. Furthermore, a possible DJ1 target mRNA, CACNA2D2, is also aberrantly expressed at the synapses in AD, both at the mRNA and protein level. This work suggests that DJ1 may be a possible target upstream of L-type voltage-gated calcium channels (VGCC), and hence pose as a novel target for treatment of MCI, correcting calcium dynamics at the synapse.","abstract_has_math":false,"creators":["Uneri, Ayse"],"institution":"Wake Forest University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018","date_published":"2018","updated_at":"2026-07-27T22:02:17Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10339/90732","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Uneri, Ayse"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-05-24T08:36:11Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2023-05-22T08:30:13Z"]},{"key":"dc:date.issued","label":"Date","values":["2018"]},{"key":"dc:publisher","label":"Institution","values":["Wake Forest University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10339/90732"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Alzheimer’s Disease (AD) is a form of dementia with aberrant synaptic transmission. It has been hypothesized that disrupted calcium signaling may cause the digression from healthy memory to mild cognitive impairment (MCI) and eventually AD; and the search for the source of calcium dyshomeostasis continues still. The goal of this thesis is to take a bottom up approach to understanding the role of calcium in cellular memory during healthy and disease states. The mammalian target of rapamycin (mTOR) is a ubiquitously expressed kinase that governs protein synthesis, and is required for learning and memory. Here, we explore a downstream target of mTOR, the novel RNA-binding protein (RBP) Parkinson Protein 7 (DJ1) (Niere et al. 2016). Our data suggests, for the first time, that DJ1 is aberrantly expressed at the synaptic level in AD. Furthermore, a possible DJ1 target mRNA, CACNA2D2, is also aberrantly expressed at the synapses in AD, both at the mRNA and protein level. This work suggests that DJ1 may be a possible target upstream of L-type voltage-gated calcium channels (VGCC), and hence pose as a novel target for treatment of MCI, correcting calcium dynamics at the synapse."]},{"key":"dc:title","label":"Title","values":["Dysregulated DJ1-Mediated Translation in Alzheimer's Disease"]}]}],"canonical_facts":{"dc:creator":["Uneri, Ayse"],"dc:date.accessioned":["2018-05-24T08:36:11Z"],"dc:date.available":["2023-05-22T08:30:13Z"],"dc:date.issued":["2018"],"dc:description.abstract":["Alzheimer’s Disease (AD) is a form of dementia with aberrant synaptic transmission. It has been hypothesized that disrupted calcium signaling may cause the digression from healthy memory to mild cognitive impairment (MCI) and eventually AD; and the search for the source of calcium dyshomeostasis continues still. The goal of this thesis is to take a bottom up approach to understanding the role of calcium in cellular memory during healthy and disease states. The mammalian target of rapamycin (mTOR) is a ubiquitously expressed kinase that governs protein synthesis, and is required for learning and memory. Here, we explore a downstream target of mTOR, the novel RNA-binding protein (RBP) Parkinson Protein 7 (DJ1) (Niere et al. 2016). Our data suggests, for the first time, that DJ1 is aberrantly expressed at the synaptic level in AD. Furthermore, a possible DJ1 target mRNA, CACNA2D2, is also aberrantly expressed at the synapses in AD, both at the mRNA and protein level. This work suggests that DJ1 may be a possible target upstream of L-type voltage-gated calcium channels (VGCC), and hence pose as a novel target for treatment of MCI, correcting calcium dynamics at the synapse."],"dc:identifier.uri":["http://hdl.handle.net/10339/90732"],"dc:language.iso":["en"],"dc:publisher":["Wake Forest University"],"dc:title":["Dysregulated DJ1-Mediated Translation in Alzheimer's Disease"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T22:02:17Z"}