IDENTIFICATION OF Cav 3.2 T-TYPE VOLTAGE GATED CALCIUM CHANNELS AS KEY MEDIATORS OF TARGETED INHIBITION OF HEPATOCELLULAR CARCINOMA BY AMPLITUDE-MODULATED RADIOFREQUENCY ELECTROMAGNETIC FIELDS

Amplitude - modulated 27.12 MHz radiofrequency electromagnetic fields (AM RF EMF) delivered via a spoon - shaped antenna placed on the patient’s tongue results in shrinkage of the primary and metastatic tumors in patients with advanced hepatocellular carcinoma (HCC). The mechanism by which AM RF EMF have direct antiproliferative eff ect ca ncer cells is largely unknown. In order to deduce the unknown mechanism of action w e have a ssessed the Specific Absorption Rate (SAR) level and dis tribution inside the human body . Additionally, we performed various i n vitro and in vivo experiments that were exposed to HCC - specific AM RF EMF using systems replicating human exposure levels and treatment duration . The resulting data provided new insights into the mechanism of action of a novel systemic therapy. Therefore, this dissertation will show that HCC - specific AM RF EMF’s antiproliferative effects are mediated by Ca 2+ influx through Ca v 3.2 T - type voltage - gated calcium channel (CACNA1H). This finding establish es a key functional role for Ca v 3.2 in control of HCC proliferation, and furthermore establishes this transmembrane protein complex as a main target for HCC - specific AM RF EMF.

Grantor dc:publisher

Wake Forest University

Year dc:date.issued

2018

Author dc:creator

• Jimenez, Hugo

• Amplitude-modulated

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/10339/90681

OAI identifier oai:identifier

oai:wakespace.lib.wfu.edu:10339/90681