{"id":{"repo_id":"wfu","oai_identifier":"oai:wakespace.lib.wfu.edu:10339/86332"},"canonical_url":"https://search.dev.ndltd.org/etd/wfu/oai:wakespace.lib.wfu.edu:10339/86332","repository":{"repo_id":"wfu","name":"Wake Forest University","base_url":"https://wakespace.lib.wfu.edu/oai/request"},"display":{"title":"ESTABLISHING A MODEL OF ORGAN REGENERATION IN THE YOUNG MAMMAL: Manipulating and Developing a Permissive Microenvironment","abstract":"The repair potential of the mammalian bladder has been known for over a century, but very few studies have examined the extent of bladder regeneration using animal models. The aim of the present studies was to develop a model of bladder regeneration in mice with specific emphasis on restoration of function. The impacts of immune response and a permissive microenvironment were also evaluated. Removal of a large portion of the urinary bladder (subtotal cystectomy; STC) in adult mice initiated a proliferative response that eventually gave rise to normal bladder wall architecture, including nerves, vessels, urothelium, and smooth muscle. Regenerated bladders displayed a normal low pressure, high capacity function, and were able to empty completely 12 weeks after cystectomy. Magnetic Resonance Imaging revealed a progressive increase in the bladder capacity and was able to predict the rate of bladder growth that resulted in normal function. InCITE, a combined optical tissue clearing method and computational analysis approach enabled a qualitative and quantitative exploration of the relationship between smooth muscle cell and extracellular matrix fiber morphometrics and functional recovery during repair.","abstract_html":"The repair potential of the mammalian bladder has been known for over a century, but very few studies have examined the extent of bladder regeneration using animal models. The aim of the present studies was to develop a model of bladder regeneration in mice with specific emphasis on restoration of function. The impacts of immune response and a permissive microenvironment were also evaluated. Removal of a large portion of the urinary bladder (subtotal cystectomy; STC) in adult mice initiated a proliferative response that eventually gave rise to normal bladder wall architecture, including nerves, vessels, urothelium, and smooth muscle. Regenerated bladders displayed a normal low pressure, high capacity function, and were able to empty completely 12 weeks after cystectomy. Magnetic Resonance Imaging revealed a progressive increase in the bladder capacity and was able to predict the rate of bladder growth that resulted in normal function. InCITE, a combined optical tissue clearing method and computational analysis approach enabled a qualitative and quantitative exploration of the relationship between smooth muscle cell and extracellular matrix fiber morphometrics and functional recovery during repair.","abstract_has_math":false,"creators":["Zarifpour, Mona"],"institution":"Wake Forest University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-27T22:02:17Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10339/86332","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Zarifpour, Mona"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-08-22T08:35:19Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2019-08-21T08:30:10Z"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:publisher","label":"Institution","values":["Wake Forest University"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10339/86332"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The repair potential of the mammalian bladder has been known for over a century, but very few studies have examined the extent of bladder regeneration using animal models. The aim of the present studies was to develop a model of bladder regeneration in mice with specific emphasis on restoration of function. The impacts of immune response and a permissive microenvironment were also evaluated. Removal of a large portion of the urinary bladder (subtotal cystectomy; STC) in adult mice initiated a proliferative response that eventually gave rise to normal bladder wall architecture, including nerves, vessels, urothelium, and smooth muscle. Regenerated bladders displayed a normal low pressure, high capacity function, and were able to empty completely 12 weeks after cystectomy. Magnetic Resonance Imaging revealed a progressive increase in the bladder capacity and was able to predict the rate of bladder growth that resulted in normal function. InCITE, a combined optical tissue clearing method and computational analysis approach enabled a qualitative and quantitative exploration of the relationship between smooth muscle cell and extracellular matrix fiber morphometrics and functional recovery during repair."]},{"key":"dc:title","label":"Title","values":["ESTABLISHING A MODEL OF ORGAN REGENERATION IN THE YOUNG MAMMAL: Manipulating and Developing a Permissive Microenvironment"]}]}],"canonical_facts":{"dc:creator":["Zarifpour, Mona"],"dc:date.accessioned":["2017-08-22T08:35:19Z"],"dc:date.available":["2019-08-21T08:30:10Z"],"dc:date.issued":["2017"],"dc:description.abstract":["The repair potential of the mammalian bladder has been known for over a century, but very few studies have examined the extent of bladder regeneration using animal models. The aim of the present studies was to develop a model of bladder regeneration in mice with specific emphasis on restoration of function. The impacts of immune response and a permissive microenvironment were also evaluated. Removal of a large portion of the urinary bladder (subtotal cystectomy; STC) in adult mice initiated a proliferative response that eventually gave rise to normal bladder wall architecture, including nerves, vessels, urothelium, and smooth muscle. Regenerated bladders displayed a normal low pressure, high capacity function, and were able to empty completely 12 weeks after cystectomy. Magnetic Resonance Imaging revealed a progressive increase in the bladder capacity and was able to predict the rate of bladder growth that resulted in normal function. InCITE, a combined optical tissue clearing method and computational analysis approach enabled a qualitative and quantitative exploration of the relationship between smooth muscle cell and extracellular matrix fiber morphometrics and functional recovery during repair."],"dc:identifier.uri":["http://hdl.handle.net/10339/86332"],"dc:language.iso":["en"],"dc:publisher":["Wake Forest University"],"dc:title":["ESTABLISHING A MODEL OF ORGAN REGENERATION IN THE YOUNG MAMMAL: Manipulating and Developing a Permissive Microenvironment"],"dc:type":["Dissertation"]},"updated_at":"2026-07-27T22:02:17Z"}