{"id":{"repo_id":"wfu","oai_identifier":"oai:wakespace.lib.wfu.edu:10339/82212"},"canonical_url":"https://search.dev.ndltd.org/etd/wfu/oai:wakespace.lib.wfu.edu:10339/82212","repository":{"repo_id":"wfu","name":"Wake Forest University","base_url":"https://wakespace.lib.wfu.edu/oai/request"},"display":{"title":"Interaction between RSNO and H2S: The formation, stability, and NO-donating capacity of SSNO- and the effect of SSNO- on platelet activation","abstract":"H2S is a gaseous mediator that has its own beneficial effects on vascular physiology. It has been implicated in the modulation of cellular processes including vessel tone/constriction, blood pressure, and tissue reperfusion. When S-nitrosothiols (RSNOs) react with excess H2S, they form several intermediates including the soluble guanylate cyclase (sGC) activating nitrosopersulfide (SSNO-). However, the stability and reactivity of this intermediate remained under debate, and hence, the relevance of nitric oxide (NO) and HS- reactions had not been established. With novel and confirmatory data, my work has demonstrated the capacity of SSNO- to donate NO and the mechanism by which it does so. I have shown that SSNO- has a half-life in anaerobic and aqueous conditions of 40 minutes. I employed UV/visible spectroscopy, electron paramagnetic resonance (EPR), and took advantage of the redox reactivity of liganded hemoglobin under aerobic and anaerobic conditions, to determine that SSNO- spontaneously releases very little NO upon decomposition. Using the selectivity of ferricatalase, I have also shown that nitroxyl (HNO) release from SSNO- decomposition is unlikely. However, I have determined that NO is acquired from SSNO- in the presence of vacant heme. Indeed, our data shows that a vacant heme is necessary to acquire NO from SSNO-, which indicates a direct heme-SSNO- reaction. SSNO- reacts with ferrous and ferri-hemes, but more efficiently with ferrihemoglobin (metHb). I have further demonstrated the stability of SSNO- in platelet-rich plasma, in which, the inhibition of platelet activation in platelets treated with SSNO- is comparable to those treated with S-nitrosoglutathione (GSNO).","abstract_html":"H2S is a gaseous mediator that has its own beneficial effects on vascular physiology. It has been implicated in the modulation of cellular processes including vessel tone/constriction, blood pressure, and tissue reperfusion. When S-nitrosothiols (RSNOs) react with excess H2S, they form several intermediates including the soluble guanylate cyclase (sGC) activating nitrosopersulfide (SSNO-). However, the stability and reactivity of this intermediate remained under debate, and hence, the relevance of nitric oxide (NO) and HS- reactions had not been established. With novel and confirmatory data, my work has demonstrated the capacity of SSNO- to donate NO and the mechanism by which it does so. I have shown that SSNO- has a half-life in anaerobic and aqueous conditions of 40 minutes. I employed UV/visible spectroscopy, electron paramagnetic resonance (EPR), and took advantage of the redox reactivity of liganded hemoglobin under aerobic and anaerobic conditions, to determine that SSNO- spontaneously releases very little NO upon decomposition. Using the selectivity of ferricatalase, I have also shown that nitroxyl (HNO) release from SSNO- decomposition is unlikely. However, I have determined that NO is acquired from SSNO- in the presence of vacant heme. Indeed, our data shows that a vacant heme is necessary to acquire NO from SSNO-, which indicates a direct heme-SSNO- reaction. SSNO- reacts with ferrous and ferri-hemes, but more efficiently with ferrihemoglobin (metHb). I have further demonstrated the stability of SSNO- in platelet-rich plasma, in which, the inhibition of platelet activation in platelets treated with SSNO- is comparable to those treated with S-nitrosoglutathione (GSNO).","abstract_has_math":false,"creators":["Bolden, Crystal Antoinette"],"institution":"Wake Forest University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-27T22:02:11Z","subjects":["Hydrogen sulfide"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10339/82212","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Bolden, Crystal Antoinette"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-06-15T08:36:02Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2019-06-14T08:30:14Z"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:publisher","label":"Institution","values":["Wake Forest University"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Hydrogen sulfide"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10339/82212"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["H2S is a gaseous mediator that has its own beneficial effects on vascular physiology. It has been implicated in the modulation of cellular processes including vessel tone/constriction, blood pressure, and tissue reperfusion. When S-nitrosothiols (RSNOs) react with excess H2S, they form several intermediates including the soluble guanylate cyclase (sGC) activating nitrosopersulfide (SSNO-). However, the stability and reactivity of this intermediate remained under debate, and hence, the relevance of nitric oxide (NO) and HS- reactions had not been established. With novel and confirmatory data, my work has demonstrated the capacity of SSNO- to donate NO and the mechanism by which it does so. I have shown that SSNO- has a half-life in anaerobic and aqueous conditions of 40 minutes. I employed UV/visible spectroscopy, electron paramagnetic resonance (EPR), and took advantage of the redox reactivity of liganded hemoglobin under aerobic and anaerobic conditions, to determine that SSNO- spontaneously releases very little NO upon decomposition. Using the selectivity of ferricatalase, I have also shown that nitroxyl (HNO) release from SSNO- decomposition is unlikely. However, I have determined that NO is acquired from SSNO- in the presence of vacant heme. Indeed, our data shows that a vacant heme is necessary to acquire NO from SSNO-, which indicates a direct heme-SSNO- reaction. SSNO- reacts with ferrous and ferri-hemes, but more efficiently with ferrihemoglobin (metHb). I have further demonstrated the stability of SSNO- in platelet-rich plasma, in which, the inhibition of platelet activation in platelets treated with SSNO- is comparable to those treated with S-nitrosoglutathione (GSNO)."]},{"key":"dc:title","label":"Title","values":["Interaction between RSNO and H2S: The formation, stability, and NO-donating capacity of SSNO- and the effect of SSNO- on platelet activation"]}]}],"canonical_facts":{"dc:creator":["Bolden, Crystal Antoinette"],"dc:date.accessioned":["2017-06-15T08:36:02Z"],"dc:date.available":["2019-06-14T08:30:14Z"],"dc:date.issued":["2017"],"dc:description.abstract":["H2S is a gaseous mediator that has its own beneficial effects on vascular physiology. It has been implicated in the modulation of cellular processes including vessel tone/constriction, blood pressure, and tissue reperfusion. When S-nitrosothiols (RSNOs) react with excess H2S, they form several intermediates including the soluble guanylate cyclase (sGC) activating nitrosopersulfide (SSNO-). However, the stability and reactivity of this intermediate remained under debate, and hence, the relevance of nitric oxide (NO) and HS- reactions had not been established. With novel and confirmatory data, my work has demonstrated the capacity of SSNO- to donate NO and the mechanism by which it does so. I have shown that SSNO- has a half-life in anaerobic and aqueous conditions of 40 minutes. I employed UV/visible spectroscopy, electron paramagnetic resonance (EPR), and took advantage of the redox reactivity of liganded hemoglobin under aerobic and anaerobic conditions, to determine that SSNO- spontaneously releases very little NO upon decomposition. Using the selectivity of ferricatalase, I have also shown that nitroxyl (HNO) release from SSNO- decomposition is unlikely. However, I have determined that NO is acquired from SSNO- in the presence of vacant heme. Indeed, our data shows that a vacant heme is necessary to acquire NO from SSNO-, which indicates a direct heme-SSNO- reaction. SSNO- reacts with ferrous and ferri-hemes, but more efficiently with ferrihemoglobin (metHb). I have further demonstrated the stability of SSNO- in platelet-rich plasma, in which, the inhibition of platelet activation in platelets treated with SSNO- is comparable to those treated with S-nitrosoglutathione (GSNO)."],"dc:identifier.uri":["http://hdl.handle.net/10339/82212"],"dc:language.iso":["en"],"dc:publisher":["Wake Forest University"],"dc:subject":["Hydrogen sulfide"],"dc:title":["Interaction between RSNO and H2S: The formation, stability, and NO-donating capacity of SSNO- and the effect of SSNO- on platelet activation"],"dc:type":["Dissertation"]},"updated_at":"2026-07-27T22:02:11Z"}