{"id":{"repo_id":"wfu","oai_identifier":"oai:wakespace.lib.wfu.edu:10339/14798"},"canonical_url":"https://search.dev.ndltd.org/etd/wfu/oai:wakespace.lib.wfu.edu:10339/14798","repository":{"repo_id":"wfu","name":"Wake Forest University","base_url":"https://wakespace.lib.wfu.edu/oai/request"},"display":{"title":"INVESTIGATING THE FUNCTIONAL ROLES OF CRF-BINDING PROTEIN HOMOLOGUE IN THE RESPONSE TO PHYSIOLOGICAL STRESSES IN DROSOPHILA","abstract":"Stress causes a characteristic set of physiological and behavioral responses in all animals. The mammalian hormone, corticotropin-releasing factor (CRF), coordinates these stress responses to a variety of different stresses. CRF has three binding partners, including two receptors and a binding protein, and it is the receptors that initiate signaling pathways ultimately responsible for alterations in behavioral and physiological program. In contrast, the binding protein functions as a negative regulator of CRF signaling and is a critical element in coordinating dynamic aspects of stress responses. In Drosophila, the DH44 hormone is homologous to CRF and binds to two CRF-related receptors, DH44-R1 and DH44-R2. We suspect, based on this homology, that DH44 is critical in modulating the Drosophila stress response. We have identified a CRF-BP homolog in Drosophila, encoded by the gene CG15537. CG15537 possesses 60% sequence similarity to mammalian CRF-BP. Various stressors alter CRF-BP expression and, we observe changes in CG15537 expression coincident with the presentation of different stressors. Similar to CRF-BP expression in mammals, CG15537 is widely expressed throughout the Drosophila CNS. Furthermore, DH44 and its putative binding protein are co-localized in the Drosophila adult brain, which parallels the observations that CRF-BP is co-localized with CRF in the mammalian CNS. We find that CG15537 functions as a DH44-BP, through direct assessment of binding. Increased expression levels of DH44-BP causes increased survival under three different stresses. Surprisingly, decreased expression of DH44-BP also leads to enhanced survival under stress. Animals with either increased or decreased expression levels have similar behavioral phenotypes, including reduced starvation-induced hyperactivity and repressed reproductive output. Furthermore, we propose a model which reconciles the identical phenotype, and predicts that DH44-BP expression levels both is required for the onset and termination of the stress responses.","abstract_html":"Stress causes a characteristic set of physiological and behavioral responses in all animals. The mammalian hormone, corticotropin-releasing factor (CRF), coordinates these stress responses to a variety of different stresses. CRF has three binding partners, including two receptors and a binding protein, and it is the receptors that initiate signaling pathways ultimately responsible for alterations in behavioral and physiological program. In contrast, the binding protein functions as a negative regulator of CRF signaling and is a critical element in coordinating dynamic aspects of stress responses. In Drosophila, the DH44 hormone is homologous to CRF and binds to two CRF-related receptors, DH44-R1 and DH44-R2. We suspect, based on this homology, that DH44 is critical in modulating the Drosophila stress response. We have identified a CRF-BP homolog in Drosophila, encoded by the gene CG15537. CG15537 possesses 60% sequence similarity to mammalian CRF-BP. Various stressors alter CRF-BP expression and, we observe changes in CG15537 expression coincident with the presentation of different stressors. Similar to CRF-BP expression in mammals, CG15537 is widely expressed throughout the Drosophila CNS. Furthermore, DH44 and its putative binding protein are co-localized in the Drosophila adult brain, which parallels the observations that CRF-BP is co-localized with CRF in the mammalian CNS. We find that CG15537 functions as a DH44-BP, through direct assessment of binding. Increased expression levels of DH44-BP causes increased survival under three different stresses. Surprisingly, decreased expression of DH44-BP also leads to enhanced survival under stress. Animals with either increased or decreased expression levels have similar behavioral phenotypes, including reduced starvation-induced hyperactivity and repressed reproductive output. Furthermore, we propose a model which reconciles the identical phenotype, and predicts that DH44-BP expression levels both is required for the onset and termination of the stress responses.","abstract_has_math":false,"creators":["Hector, Clare"],"institution":"Wake Forest University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-05-07T18:55:15Z","date_published":"2010-05-07T18:55:15Z","updated_at":"2026-07-27T22:01:01Z","subjects":["Drosophila"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10339/14798","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Hector, Clare"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2010-05-07T18:55:15Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2010-05-07T18:55:15Z"]},{"key":"dc:date.issued","label":"Date","values":["2010-05-07T18:55:15Z"]},{"key":"dc:publisher","label":"Institution","values":["Wake Forest University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Drosophila"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10339/14798"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Stress causes a characteristic set of physiological and behavioral responses in all animals. The mammalian hormone, corticotropin-releasing factor (CRF), coordinates these stress responses to a variety of different stresses. CRF has three binding partners, including two receptors and a binding protein, and it is the receptors that initiate signaling pathways ultimately responsible for alterations in behavioral and physiological program. In contrast, the binding protein functions as a negative regulator of CRF signaling and is a critical element in coordinating dynamic aspects of stress responses. In Drosophila, the DH44 hormone is homologous to CRF and binds to two CRF-related receptors, DH44-R1 and DH44-R2. We suspect, based on this homology, that DH44 is critical in modulating the Drosophila stress response. We have identified a CRF-BP homolog in Drosophila, encoded by the gene CG15537. CG15537 possesses 60% sequence similarity to mammalian CRF-BP. Various stressors alter CRF-BP expression and, we observe changes in CG15537 expression coincident with the presentation of different stressors. Similar to CRF-BP expression in mammals, CG15537 is widely expressed throughout the Drosophila CNS. Furthermore, DH44 and its putative binding protein are co-localized in the Drosophila adult brain, which parallels the observations that CRF-BP is co-localized with CRF in the mammalian CNS. We find that CG15537 functions as a DH44-BP, through direct assessment of binding. Increased expression levels of DH44-BP causes increased survival under three different stresses. Surprisingly, decreased expression of DH44-BP also leads to enhanced survival under stress. Animals with either increased or decreased expression levels have similar behavioral phenotypes, including reduced starvation-induced hyperactivity and repressed reproductive output. Furthermore, we propose a model which reconciles the identical phenotype, and predicts that DH44-BP expression levels both is required for the onset and termination of the stress responses."]},{"key":"dc:title","label":"Title","values":["INVESTIGATING THE FUNCTIONAL ROLES OF CRF-BINDING PROTEIN HOMOLOGUE IN THE RESPONSE TO PHYSIOLOGICAL STRESSES IN DROSOPHILA"]}]}],"canonical_facts":{"dc:creator":["Hector, Clare"],"dc:date.accessioned":["2010-05-07T18:55:15Z"],"dc:date.available":["2010-05-07T18:55:15Z"],"dc:date.issued":["2010-05-07T18:55:15Z"],"dc:description.abstract":["Stress causes a characteristic set of physiological and behavioral responses in all animals. The mammalian hormone, corticotropin-releasing factor (CRF), coordinates these stress responses to a variety of different stresses. CRF has three binding partners, including two receptors and a binding protein, and it is the receptors that initiate signaling pathways ultimately responsible for alterations in behavioral and physiological program. In contrast, the binding protein functions as a negative regulator of CRF signaling and is a critical element in coordinating dynamic aspects of stress responses. In Drosophila, the DH44 hormone is homologous to CRF and binds to two CRF-related receptors, DH44-R1 and DH44-R2. We suspect, based on this homology, that DH44 is critical in modulating the Drosophila stress response. We have identified a CRF-BP homolog in Drosophila, encoded by the gene CG15537. CG15537 possesses 60% sequence similarity to mammalian CRF-BP. Various stressors alter CRF-BP expression and, we observe changes in CG15537 expression coincident with the presentation of different stressors. Similar to CRF-BP expression in mammals, CG15537 is widely expressed throughout the Drosophila CNS. Furthermore, DH44 and its putative binding protein are co-localized in the Drosophila adult brain, which parallels the observations that CRF-BP is co-localized with CRF in the mammalian CNS. We find that CG15537 functions as a DH44-BP, through direct assessment of binding. Increased expression levels of DH44-BP causes increased survival under three different stresses. Surprisingly, decreased expression of DH44-BP also leads to enhanced survival under stress. Animals with either increased or decreased expression levels have similar behavioral phenotypes, including reduced starvation-induced hyperactivity and repressed reproductive output. Furthermore, we propose a model which reconciles the identical phenotype, and predicts that DH44-BP expression levels both is required for the onset and termination of the stress responses."],"dc:identifier.uri":["http://hdl.handle.net/10339/14798"],"dc:language.iso":["en_US"],"dc:publisher":["Wake Forest University"],"dc:subject":["Drosophila"],"dc:title":["INVESTIGATING THE FUNCTIONAL ROLES OF CRF-BINDING PROTEIN HOMOLOGUE IN THE RESPONSE TO PHYSIOLOGICAL STRESSES IN DROSOPHILA"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T22:01:01Z"}