Production of Polyhydroxyalkanoates and their application for coronary stent development
type: CreativeWork
description: Development of coronary artery stents have revolutionised the treatment of coronary artery disease. However, the currently available stents have several limitations and side effects, which still need to be addressed. Within this group of biomaterials Polyhydroxyalkanoates (PHAs), which are biopolyesters produced by bacteria, have a great potential in coronary stent development. They are biodegradable, non-toxic and biocompatible. PHAs have a broad range of properties, which can be tailored based on the requirements of the application by an appropriate bacterial strain, carbon source or fermentation conditions. The main aim of this study was the production of PHAs and their application as a platform material for coronary stent. Poly(3-hydroxybutyrate), P(3HB), short chain length PHA (scl-PHA), was obtained from B.subtilis OK2 using glucose as the sole carbon source. P. mendocina was selected for the economical production of medium chain length PHA (mcl-PHAs) using waste frying oil as the carbon source. Produced mcl-PHA copolymer was hydrolysed in order to obtain lower molecular weight PHA (oligo-PHA), which was used as the plasticising agent for P(3HB) in order to alter material properties of the scl-PHA polymer. Novel P(3HB)/oligo-PHA blends, using different ratios of P(3HB) and oligo-PHA, which were 100:0 (P(3HB), 95:5 (P(3HB): oligo-PHA), 90:10 (P(3HB): oligo-PHA) and 80:20 (P(3HB): oligo-PHA) have been developed. Addition of oligo-PHA to P(3HB) resulted not only in the enhancement of mechanical properties of the material, but also improved surface topography and increased the biocompatibility of the scaffolds in comparison to neat P(3HB). The P(3HB)/oligo-PHA 90/10 blend was selected for the fabrication of radiopaque composites using addition of 1%, 3% and 5% wt barium sulphate. BaSO4 is well-known contrast agent, commonly used in biomedical applications. The composites were fully characterised with respect to material properties, surface topography and biocompat…
abstract: Development of coronary artery stents have revolutionised the treatment of coronary artery disease. However, the currently available stents have several limitations and side effects, which still need to be addressed. Within this group of biomaterials Polyhydroxyalkanoates (PHAs), which are biopolyesters produced by bacteria, have a great potential in coronary stent development. They are biodegradable, non-toxic and biocompatible. PHAs have a broad range of properties, which can be tailored based on the requirements of the application by an appropriate bacterial strain, carbon source or fermentation conditions. The main aim of this study was the production of PHAs and their application as a platform material for coronary stent. Poly(3-hydroxybutyrate), P(3HB), short chain length PHA (scl-PHA), was obtained from B.subtilis OK2 using glucose as the sole carbon source. P. mendocina was selected for the economical production of medium chain length PHA (mcl-PHAs) using waste frying oil as the carbon source. Produced mcl-PHA copolymer was hydrolysed in order to obtain lower molecular weight PHA (oligo-PHA), which was used as the plasticising agent for P(3HB) in order to alter material properties of the scl-PHA polymer. Novel P(3HB)/oligo-PHA blends, using different ratios of P(3HB) and oligo-PHA, which were 100:0 (P(3HB), 95:5 (P(3HB): oligo-PHA), 90:10 (P(3HB): oligo-PHA) and 80:20 (P(3HB): oligo-PHA) have been developed. Addition of oligo-PHA to P(3HB) resulted not only in the enhancement of mechanical properties of the material, but also improved surface topography and increased the biocompatibility of the scaffolds in comparison to neat P(3HB). The P(3HB)/oligo-PHA 90/10 blend was selected for the fabrication of radiopaque composites using addition of 1%, 3% and 5% wt barium sulphate. BaSO4 is well-known contrast agent, commonly used in biomedical applications. The composites were fully characterised with respect to material properties, surface topography and biocompat…
author: Lukasiewicz, B.
date published: 2017
date modified: 2026-07-24T06:01:42Z
provider: University of Westminster
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Westminster

PropertyValue
property ID: DOI
value: 10.34737/q50q0