Cytochrome C biosensor for the determination of trace level arsenic and cyanide compounds
type: CreativeWork
description: In this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 Ã—10-9 M) and the detection limits ranging from 0.0043- 9.1 Î¼M. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines.
abstract: In this work, an electrochemical method based on a cyt c biosensor has been developed, for the detection of selected arsenic and cyanide compounds. Boron Doped Diamond (BDD) electrode was used as a transducer, onto which cyt c was immobilised and used for direct determination of Prussian blue, potassium cyanide and arsenic trioxide by inhibition mechanism. The sensitivity as calculated from cyclic voltammetry (CV) and square wave voltammetry (SWV), for each analyte in phosphate buffer (pH= 7) was found to be (1.087- 4.488 Ã—10-9 M) and the detection limits ranging from 0.0043- 9.1 Î¼M. These values represent a big improvement over the current Environmental Protection Agency (EPA) and World Health Organisation (WHO) guidelines.
author: Fuku, Xolile Godfrey
keywords: Cytochrome c (cyt c), Boron doped diamond (BDD), Prussian blue (PB), Arsenic trioxide (As), Incubation, Drop coating, Intoxication, Toxicity, Electron-transport chain, Asphyxiates, Cyclic voltammetry (CV), Square wave voltammetry (SWV), Electrochemical impedance spectroscopy (EIS), Fourier Transform Infrared Spectroscopy (FTIR), UV/vis spectroscopy, Subtractive Normalization Fourier Transform Infrared Spectroscopy (SNFTIR)
date published: 2011
date modified: 2026-07-24T06:00:46Z
provider: University of the Western Cape
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of the Western Cape