Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial drugs. The ability of compounds to withstand environmental stress and storage were explored with only one compound showing significant instability. Finally MTS assay showed that cell proliferation was decreased when mouse embryonic fibroblast cells, NIH 3T3 were treated with the compounds, proving they are cytotoxic to varying degrees.
","abstract_html":"<p>Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial drugs. The ability of compounds to withstand environmental stress and storage were explored with only one compound showing significant instability. Finally MTS assay showed that cell proliferation was decreased when mouse embryonic fibroblast cells, NIH 3T3 were treated with the compounds, proving they are cytotoxic to varying degrees.</p>","abstract_has_math":false,"creators":["Canning, Corene Barbara"],"institution":null,"degree_name":"Ph.D.","degree_level":"Open Access Dissertation","degree_discipline":"Nutrition and Food Science","degree_department":null,"school":null,"contributors":["Kequan (Kevin) Zhou"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-01-01T08:00:00Z","date_published":"2014-01-01T08:00:00Z","updated_at":"2026-07-24T05:59:33Z","subjects":["antimicrobial resistance","high priority pathogens","marine organism","natural antimicrobials","plants","Food Science"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.wayne.edu/oa_dissertations/875","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kequan (Kevin) Zhou"]},{"key":"dc:creator","label":"Author","values":["Canning, Corene Barbara"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2014-01-01T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutrition and Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["antimicrobial resistance","high priority pathogens","marine organism","natural antimicrobials","plants","Food Science"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.wayne.edu/oa_dissertations/875"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial drugs. The ability of compounds to withstand environmental stress and storage were explored with only one compound showing significant instability. Finally MTS assay showed that cell proliferation was decreased when mouse embryonic fibroblast cells, NIH 3T3 were treated with the compounds, proving they are cytotoxic to varying degrees.
"]},{"key":"dc:title","label":"Title","values":["Isolation And Characterization Of Natural Antimicrobials From Plant And Marine Organisms"]}]}],"canonical_facts":{"dc:contributor":["Kequan (Kevin) Zhou"],"dc:creator":["Canning, Corene Barbara"],"dc:date.available":["2014-01-01T08:00:00Z"],"dc:description.abstract":["Antibiotic agents have been found to have a limited lifespan due to the ability of microrganisms to develop antimicrobial resistance. In this study a number extracts of plant and marine organisms were evaluated for their antimicrobial activity against a number of high priority pathogens. Bioassay-guided fractionation of four extracts led to the identification of compounds with inhibitory activity; including one previously unknown compound. Compounds were evaluated through a variety of techniques including broth microdilution assay to determine each compound's MIC and MBC. The majority of the compounds showed antimicrobial activity equal to, if not better than, the commonly used antimicrobial drugs. The ability of compounds to withstand environmental stress and storage were explored with only one compound showing significant instability. Finally MTS assay showed that cell proliferation was decreased when mouse embryonic fibroblast cells, NIH 3T3 were treated with the compounds, proving they are cytotoxic to varying degrees.
"],"dc:identifier":["https://digitalcommons.wayne.edu/oa_dissertations/875"],"dc:subject":["antimicrobial resistance","high priority pathogens","marine organism","natural antimicrobials","plants","Food Science"],"dc:title":["Isolation And Characterization Of Natural Antimicrobials From Plant And Marine Organisms"],"thesis:degree_discipline":["Nutrition and Food Science"],"thesis:degree_level":["Open Access Dissertation"],"thesis:degree_name":["Ph.D."]},"updated_at":"2026-07-24T05:59:33Z"}