Characterization Of High-Voltage-Activated Calcium Channels In Retinal Bipolar Cells

<p>Retinal bipolar cells, conveying visual information from photoreceptors to ganglion cells, segregate visual information into multiple parallel pathways through their diversified cell types and physiological properties. Voltage-gated Ca²⁺ channels could be particularly important underlying the diversified physiological properties of different BCs. In this dissertation, I investigated the high-voltage-activated (HVA) calcium current in retinal bipolar cells in mice. In the first part of my dissertation, I characterized multiple bipolar cell-expressing GFP and/or Cre transgenic mouse lines. In the second part of my dissertation, by performing whole-cell patch-clamp recordings, I examined the electrophysiological properties of HVA calcium currents among CBCs and between CBCs and CBCs. In particular, the second part of my study focused on the investigation of electrophysiological, pharmacological, and molecular properties of HVA calcium currents in RBCs. The results of my studies showed that the HVA Ca²⁺ currents with different electrophysiological properties were observed among CBCs, and between CBCs and RBCs. First, large HVA Ca²⁺ currents were observed in OFF CBCs but not in ON-CBCs. Second, HVA Ca²⁺ currents among different bipolar cells were found to show different activation potentials. Furthermore, the HVA Ca²⁺ currents in RBCs exhibited two components, a sustained and a transient component with the latter activated at more negative potentials. My pharmacological results indicated the sustained and transient HVA Ca²⁺ currents are originated from L- and P/Q type Ca²⁺ channels, respectively. Using L-type Ca²⁺ channel knockout or deficient mouse lines, my results showed that the L-type Ca²⁺ currents in RBCs are mediated mainly by alpha1C Ca²⁺ channels with a minor component from alpha1F Ca²⁺ channels. The studies will advance our understanding of the role of voltage-gated Ca²⁺ channels in basic visual information processing in the retina as well as Ca²⁺ signaling and Ca²⁺ channel deficit-related diseases in the visual system.</p>

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Open Access Dissertation

Discipline thesis:degree_discipline

Anatomy and Cell Biology

Year dc:date.available

2013

Author dc:creator

• Lu, Qi

Contributors dc:contributor

• Zhuo-Hua Pan

• L-type calcium channel
• P/Q-type calcium channel
• retina bipolar cell
• type 4 cone bipolar cell
• voltage gated calcium channel
• Cell Biology
• Ophthalmology
• Physiology

Repository record dc:identifier

https://digitalcommons.wayne.edu/oa_dissertations/783

OAI identifier oai:identifier

oai:digitalcommons.wayne.edu:oa_dissertations-1782