{"id":{"repo_id":"washington","oai_identifier":"oai:digital.lib.washington.edu:1773/53945"},"canonical_url":"https://search.dev.ndltd.org/etd/washington/oai:digital.lib.washington.edu:1773/53945","repository":{"repo_id":"washington","name":"University of Washington","base_url":"https://digital.lib.washington.edu/server/oai/request"},"display":{"title":"Turning Tears into Sweets: CandyCollect for Pediatric Salivary Pathogen Sampling","abstract":"Open microfluidic channels are gaining widespread use in biotechnology, biology, and diagnostics due to their broad applicability and user-friendly design, which enables easy addition or removal of components. Chapter 1 reviews capillary-driven open microfluidic systems and the implementation of open microfluidic channels in helping human specimen sampling. Chapter 2 introduces an improved open microfluidic system with enhanced capillary pumping capabilities. Chapter 3 describes the use of the CandyCollect device—a lollipop-inspired, open microfluidic tool for collecting salivary pathogens—in an initial human subject study. This remote, at-home study demonstrated the CandyCollect device's ability to effectively capture commensal salivary bacteria, including Streptococcus mutans and Staphylococcus aureus, from healthy adults. Chapter 4 presents the first clinical data using CandyCollect devices to sample pediatric patients diagnosed with Group A Streptococcus (GAS) pharyngitis. In summary, this dissertation spans from the foundational design principles of open microfluidic systems to their practical application in enhancing human pathogen sampling.","abstract_html":"Open microfluidic channels are gaining widespread use in biotechnology, biology, and diagnostics due to their broad applicability and user-friendly design, which enables easy addition or removal of components. Chapter 1 reviews capillary-driven open microfluidic systems and the implementation of open microfluidic channels in helping human specimen sampling. Chapter 2 introduces an improved open microfluidic system with enhanced capillary pumping capabilities. Chapter 3 describes the use of the CandyCollect device—a lollipop-inspired, open microfluidic tool for collecting salivary pathogens—in an initial human subject study. This remote, at-home study demonstrated the CandyCollect device's ability to effectively capture commensal salivary bacteria, including Streptococcus mutans and Staphylococcus aureus, from healthy adults. Chapter 4 presents the first clinical data using CandyCollect devices to sample pediatric patients diagnosed with Group A Streptococcus (GAS) pharyngitis. In summary, this dissertation spans from the foundational design principles of open microfluidic systems to their practical application in enhancing human pathogen sampling.","abstract_has_math":false,"creators":["Tu, Wan-chen"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Theberge, Ashleigh B."],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-10-02","date_published":"2025-10-02","updated_at":"2026-07-24T05:58:01Z","subjects":["Analytical chemistry"],"languages":["en_US"],"rights":["CC BY-ND"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1773/53945","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Theberge, Ashleigh B."]},{"key":"dc:creator","label":"Author","values":["Tu, Wan-chen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-10-02T16:06:33Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-10-02T16:06:33Z"]},{"key":"dc:date.issued","label":"Date","values":["2025-10-02"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Analytical chemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["CC BY-ND"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["Tu_washington_0250E_28766.pdf"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1773/53945"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (Ph.D.)--University of Washington, 2025"]},{"key":"dc:description.abstract","label":"Abstract","values":["Open microfluidic channels are gaining widespread use in biotechnology, biology, and diagnostics due to their broad applicability and user-friendly design, which enables easy addition or removal of components. Chapter 1 reviews capillary-driven open microfluidic systems and the implementation of open microfluidic channels in helping human specimen sampling. Chapter 2 introduces an improved open microfluidic system with enhanced capillary pumping capabilities. Chapter 3 describes the use of the CandyCollect device—a lollipop-inspired, open microfluidic tool for collecting salivary pathogens—in an initial human subject study. This remote, at-home study demonstrated the CandyCollect device's ability to effectively capture commensal salivary bacteria, including Streptococcus mutans and Staphylococcus aureus, from healthy adults. Chapter 4 presents the first clinical data using CandyCollect devices to sample pediatric patients diagnosed with Group A Streptococcus (GAS) pharyngitis. 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Chapter 1 reviews capillary-driven open microfluidic systems and the implementation of open microfluidic channels in helping human specimen sampling. Chapter 2 introduces an improved open microfluidic system with enhanced capillary pumping capabilities. Chapter 3 describes the use of the CandyCollect device—a lollipop-inspired, open microfluidic tool for collecting salivary pathogens—in an initial human subject study. This remote, at-home study demonstrated the CandyCollect device's ability to effectively capture commensal salivary bacteria, including Streptococcus mutans and Staphylococcus aureus, from healthy adults. Chapter 4 presents the first clinical data using CandyCollect devices to sample pediatric patients diagnosed with Group A Streptococcus (GAS) pharyngitis. 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