{"id":{"repo_id":"washington","oai_identifier":"oai:digital.lib.washington.edu:1773/36819"},"canonical_url":"https://search.dev.ndltd.org/etd/washington/oai:digital.lib.washington.edu:1773/36819","repository":{"repo_id":"washington","name":"University of Washington","base_url":"https://digital.lib.washington.edu/server/oai/request"},"display":{"title":"The effects of noise exposure and genetic background on auditory-evoked behaviors in larval zebrafish","abstract":"Auditory sensitivity and perception can be influenced by extrinsic factors, such as environmental noise, and intrinsic factors, such as genes and arousal. How do these factors interact and influence auditory-related behaviors? I addressed this question in the larval zebrafish (Danio rerio). Zebrafish have been a powerful genetic model species used in the study of development of auditory structures and exhibit a well-studied and robust escape response when presented with a loud acoustic stimulus. I used psychophysical methods to measure auditory sensitivity and perception by observing changes in this behavioral response. In chapter 2, I describe a novel paradigm using prepulse inhibition as a tool to measure auditory sensitivity in larval zebrafish. I show that this paradigm is more sensitive than other behavioral measures used in zebrafish research. Next, in chapter 3, I demonstrate that loud noise exposure leads to a temporary hypersensitivity toward startle-inducing stimuli, whereas auditory sensitivity, as measured using the prepulse inhibition paradigm, is unchanged. I use pharmacological and physiological measures to explore potential mechanisms of this hypersensitization effect. Finally, in chapter 4, I investigate effects of genetic background on auditory sensitivity and susceptibility to noise exposure between closely related individuals and across multiple zebrafish lines. The results of these experiments show large-scale differences in startle sensitivity between wild-type zebrafish lines. I discuss the implications of this data, summarize these findings in a larger context, and discuss future studies in chapter 5.","abstract_html":"Auditory sensitivity and perception can be influenced by extrinsic factors, such as environmental noise, and intrinsic factors, such as genes and arousal. How do these factors interact and influence auditory-related behaviors? I addressed this question in the larval zebrafish (Danio rerio). Zebrafish have been a powerful genetic model species used in the study of development of auditory structures and exhibit a well-studied and robust escape response when presented with a loud acoustic stimulus. I used psychophysical methods to measure auditory sensitivity and perception by observing changes in this behavioral response. In chapter 2, I describe a novel paradigm using prepulse inhibition as a tool to measure auditory sensitivity in larval zebrafish. I show that this paradigm is more sensitive than other behavioral measures used in zebrafish research. Next, in chapter 3, I demonstrate that loud noise exposure leads to a temporary hypersensitivity toward startle-inducing stimuli, whereas auditory sensitivity, as measured using the prepulse inhibition paradigm, is unchanged. I use pharmacological and physiological measures to explore potential mechanisms of this hypersensitization effect. Finally, in chapter 4, I investigate effects of genetic background on auditory sensitivity and susceptibility to noise exposure between closely related individuals and across multiple zebrafish lines. The results of these experiments show large-scale differences in startle sensitivity between wild-type zebrafish lines. I discuss the implications of this data, summarize these findings in a larger context, and discuss future studies in chapter 5.","abstract_has_math":false,"creators":["Bhandiwad, Ashwin"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Sisneros, Joseph A"],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-07-14","date_published":"2016-07-14","updated_at":"2026-07-24T05:57:57Z","subjects":["auditory behavior","noise exposure","prepulse inhibition","zebrafish"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1773/36819","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Sisneros, Joseph A"]},{"key":"dc:creator","label":"Author","values":["Bhandiwad, Ashwin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-07-14T16:45:59Z"]},{"key":"dc:date.issued","label":"Date","values":["2016-07-14"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["auditory behavior","noise exposure","prepulse inhibition","zebrafish"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["Bhandiwad_washington_0250E_15711.pdf"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1773/36819"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (Ph.D.)--University of Washington, 2016-06"]},{"key":"dc:description.abstract","label":"Abstract","values":["Auditory sensitivity and perception can be influenced by extrinsic factors, such as environmental noise, and intrinsic factors, such as genes and arousal. How do these factors interact and influence auditory-related behaviors? I addressed this question in the larval zebrafish (Danio rerio). Zebrafish have been a powerful genetic model species used in the study of development of auditory structures and exhibit a well-studied and robust escape response when presented with a loud acoustic stimulus. I used psychophysical methods to measure auditory sensitivity and perception by observing changes in this behavioral response. In chapter 2, I describe a novel paradigm using prepulse inhibition as a tool to measure auditory sensitivity in larval zebrafish. I show that this paradigm is more sensitive than other behavioral measures used in zebrafish research. Next, in chapter 3, I demonstrate that loud noise exposure leads to a temporary hypersensitivity toward startle-inducing stimuli, whereas auditory sensitivity, as measured using the prepulse inhibition paradigm, is unchanged. I use pharmacological and physiological measures to explore potential mechanisms of this hypersensitization effect. Finally, in chapter 4, I investigate effects of genetic background on auditory sensitivity and susceptibility to noise exposure between closely related individuals and across multiple zebrafish lines. The results of these experiments show large-scale differences in startle sensitivity between wild-type zebrafish lines. I discuss the implications of this data, summarize these findings in a larger context, and discuss future studies in chapter 5."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The effects of noise exposure and genetic background on auditory-evoked behaviors in larval zebrafish"]}]}],"canonical_facts":{"dc:contributor.advisor":["Sisneros, Joseph A"],"dc:creator":["Bhandiwad, Ashwin"],"dc:date.accessioned":["2016-07-14T16:45:59Z"],"dc:date.issued":["2016-07-14"],"dc:description":["Thesis (Ph.D.)--University of Washington, 2016-06"],"dc:description.abstract":["Auditory sensitivity and perception can be influenced by extrinsic factors, such as environmental noise, and intrinsic factors, such as genes and arousal. How do these factors interact and influence auditory-related behaviors? I addressed this question in the larval zebrafish (Danio rerio). Zebrafish have been a powerful genetic model species used in the study of development of auditory structures and exhibit a well-studied and robust escape response when presented with a loud acoustic stimulus. I used psychophysical methods to measure auditory sensitivity and perception by observing changes in this behavioral response. In chapter 2, I describe a novel paradigm using prepulse inhibition as a tool to measure auditory sensitivity in larval zebrafish. I show that this paradigm is more sensitive than other behavioral measures used in zebrafish research. Next, in chapter 3, I demonstrate that loud noise exposure leads to a temporary hypersensitivity toward startle-inducing stimuli, whereas auditory sensitivity, as measured using the prepulse inhibition paradigm, is unchanged. I use pharmacological and physiological measures to explore potential mechanisms of this hypersensitization effect. Finally, in chapter 4, I investigate effects of genetic background on auditory sensitivity and susceptibility to noise exposure between closely related individuals and across multiple zebrafish lines. The results of these experiments show large-scale differences in startle sensitivity between wild-type zebrafish lines. I discuss the implications of this data, summarize these findings in a larger context, and discuss future studies in chapter 5."],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["Bhandiwad_washington_0250E_15711.pdf"],"dc:identifier.uri":["http://hdl.handle.net/1773/36819"],"dc:language.iso":["en_US"],"dc:subject":["auditory behavior","noise exposure","prepulse inhibition","zebrafish"],"dc:title":["The effects of noise exposure and genetic background on auditory-evoked behaviors in larval zebrafish"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T05:57:57Z"}