{"id":{"repo_id":"washington","oai_identifier":"oai:digital.lib.washington.edu:1773/25129"},"canonical_url":"https://search.dev.ndltd.org/etd/washington/oai:digital.lib.washington.edu:1773/25129","repository":{"repo_id":"washington","name":"University of Washington","base_url":"https://digital.lib.washington.edu/server/oai/request"},"display":{"title":"Surface Acoustic Wave Nebulization as a Mass Spectrometry Ionization Source, Characterization and Application","abstract":"This dissertation describes a novel method to generate ions for mass spectrometers that simplifies the operation and decreases the internal energy distribution of ions. The surface acoustic wave nebulization (SAWN) is a nebulization method generating both positive and negative ions from liquid phase. We have validated this method by successfully ionizing protein, peptide, lipid and other small molecules. The measured internal energy distribution of SAWN is lower compare to electrospray ionization (ESI). To improve the SAWN performance, a standing wave SAWN chip was designed and fabricated. The droplet size of standing wave SAWN was measured and is in general smaller than progressive wave SAWN. The standing wave SAWN design enhanced SAWN performance and kept its low internal energy distribution. After the characterization and improvement, we combined SAWN with digital microfluidics (DMF) for sample preparation before MS detection. DMF-SAWN platform was applied to the analysis of phosphorylated protein mixture. Peptides from all three proteins of the mixture were identified with DMF-SAWN.","abstract_html":"This dissertation describes a novel method to generate ions for mass spectrometers that simplifies the operation and decreases the internal energy distribution of ions. The surface acoustic wave nebulization (SAWN) is a nebulization method generating both positive and negative ions from liquid phase. We have validated this method by successfully ionizing protein, peptide, lipid and other small molecules. The measured internal energy distribution of SAWN is lower compare to electrospray ionization (ESI). To improve the SAWN performance, a standing wave SAWN chip was designed and fabricated. The droplet size of standing wave SAWN was measured and is in general smaller than progressive wave SAWN. The standing wave SAWN design enhanced SAWN performance and kept its low internal energy distribution. After the characterization and improvement, we combined SAWN with digital microfluidics (DMF) for sample preparation before MS detection. DMF-SAWN platform was applied to the analysis of phosphorylated protein mixture. Peptides from all three proteins of the mixture were identified with DMF-SAWN.","abstract_has_math":false,"creators":["Huang, Yue"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Tureček, Frantisek"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014-02-24","date_published":"2014-02-24","updated_at":"2026-07-24T05:57:59Z","subjects":["ambient ionization source; Digital Microfluidics; mass spectrometry; phosphorylated peptide; SAWN; Surface Acoustic Wave"],"languages":["en_US"],"rights":["Copyright is held by the individual authors."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1773/25129","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Tureček, Frantisek"]},{"key":"dc:creator","label":"Author","values":["Huang, Yue"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-02-24T18:28:23Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-12-14T17:55:52Z"]},{"key":"dc:date.issued","label":"Date","values":["2014-02-24"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ambient ionization source; Digital Microfluidics; mass spectrometry; phosphorylated peptide; SAWN; Surface Acoustic Wave"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the individual authors."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["Huang_washington_0250E_12526.pdf"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1773/25129"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Thesis (Ph.D.)--University of Washington, 2013"]},{"key":"dc:description.abstract","label":"Abstract","values":["This dissertation describes a novel method to generate ions for mass spectrometers that simplifies the operation and decreases the internal energy distribution of ions. The surface acoustic wave nebulization (SAWN) is a nebulization method generating both positive and negative ions from liquid phase. We have validated this method by successfully ionizing protein, peptide, lipid and other small molecules. The measured internal energy distribution of SAWN is lower compare to electrospray ionization (ESI). To improve the SAWN performance, a standing wave SAWN chip was designed and fabricated. The droplet size of standing wave SAWN was measured and is in general smaller than progressive wave SAWN. The standing wave SAWN design enhanced SAWN performance and kept its low internal energy distribution. After the characterization and improvement, we combined SAWN with digital microfluidics (DMF) for sample preparation before MS detection. DMF-SAWN platform was applied to the analysis of phosphorylated protein mixture. Peptides from all three proteins of the mixture were identified with DMF-SAWN."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Surface Acoustic Wave Nebulization as a Mass Spectrometry Ionization Source, Characterization and Application"]}]}],"canonical_facts":{"dc:contributor.advisor":["Tureček, Frantisek"],"dc:creator":["Huang, Yue"],"dc:date.accessioned":["2014-02-24T18:28:23Z"],"dc:date.available":["2015-12-14T17:55:52Z"],"dc:date.issued":["2014-02-24"],"dc:description":["Thesis (Ph.D.)--University of Washington, 2013"],"dc:description.abstract":["This dissertation describes a novel method to generate ions for mass spectrometers that simplifies the operation and decreases the internal energy distribution of ions. The surface acoustic wave nebulization (SAWN) is a nebulization method generating both positive and negative ions from liquid phase. We have validated this method by successfully ionizing protein, peptide, lipid and other small molecules. The measured internal energy distribution of SAWN is lower compare to electrospray ionization (ESI). To improve the SAWN performance, a standing wave SAWN chip was designed and fabricated. The droplet size of standing wave SAWN was measured and is in general smaller than progressive wave SAWN. The standing wave SAWN design enhanced SAWN performance and kept its low internal energy distribution. After the characterization and improvement, we combined SAWN with digital microfluidics (DMF) for sample preparation before MS detection. DMF-SAWN platform was applied to the analysis of phosphorylated protein mixture. Peptides from all three proteins of the mixture were identified with DMF-SAWN."],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["Huang_washington_0250E_12526.pdf"],"dc:identifier.uri":["http://hdl.handle.net/1773/25129"],"dc:language.iso":["en_US"],"dc:rights":["Copyright is held by the individual authors."],"dc:subject":["ambient ionization source; Digital Microfluidics; mass spectrometry; phosphorylated peptide; SAWN; Surface Acoustic Wave"],"dc:title":["Surface Acoustic Wave Nebulization as a Mass Spectrometry Ionization Source, Characterization and Application"],"dc:type":["Thesis"]},"updated_at":"2026-07-24T05:57:59Z"}