{"id":{"repo_id":"vu-aus","oai_identifier":"oai:eprints.vu.edu.au:15484"},"canonical_url":"https://search.dev.ndltd.org/etd/vu-aus/oai:eprints.vu.edu.au:15484","repository":{"repo_id":"vu-aus","name":"Victoria University (Australia)","base_url":"https://vuir.vu.edu.au/cgi/oai2"},"display":{"title":"Isolation of low ethanol producing yeast strains using adaptive evolution","abstract":"This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also compromises redox balance by preventing the fermentative oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the generation of genetically stable variants with increased glycerol and reduced ethanol production.","abstract_html":"This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also compromises redox balance by preventing the fermentative oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the generation of genetically stable variants with increased glycerol and reduced ethanol production.","abstract_has_math":false,"creators":["Kutyna, Dariusz Roman"],"institution":"Victoria University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-24T06:33:12Z","subjects":["0908 Food Sciences","0601 Biochemistry and Cell Biology","School of Engineering and Science"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Kutyna, Dariusz Roman"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2008"]},{"key":"dc:date.issued","label":"Date","values":["2008"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Molecular Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Victoria University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://vuir.vu.edu.au/15484/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["0908 Food Sciences","0601 Biochemistry and Cell Biology","School of Engineering and Science"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://vuir.vu.edu.au/15484/3/KUTYNA%20Dariusz-thesis_nosignature.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also compromises redox balance by preventing the fermentative oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the generation of genetically stable variants with increased glycerol and reduced ethanol production."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Isolation of low ethanol producing yeast strains using adaptive evolution"]}]}],"canonical_facts":{"dc:creator":["Kutyna, Dariusz Roman"],"dc:date":["2008"],"dc:date.issued":["2008"],"dc:description.abstract":["This thesis describes the application of a non-genetic engineering approach, adaptive evolution, to generate variants of Saccharomyces cerevisiae that produce reduced levels of ethanol relative to the strains they were derived from. Sub-lethal concentrations of sulfite, which sequesters acetaldehyde, thereby limiting ethanol production, was used as a selection pressure. However, removal of acetaldehyde not only limits ethanol production, it also compromises redox balance by preventing the fermentative oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the generation of genetically stable variants with increased glycerol and reduced ethanol production."],"dc:format":["text"],"dc:identifier.uri":["https://vuir.vu.edu.au/15484/3/KUTYNA%20Dariusz-thesis_nosignature.pdf"],"dc:language":["en"],"dc:publisher.department":["School of Molecular Sciences"],"dc:publisher.institution":["Victoria University"],"dc:relation.isreferencedby":["https://vuir.vu.edu.au/15484/"],"dc:subject":["0908 Food Sciences","0601 Biochemistry and Cell Biology","School of Engineering and Science"],"dc:title":["Isolation of low ethanol producing yeast strains using adaptive evolution"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T06:33:12Z"}