Characterizing Interfacial and Bulk Interactions Between Cellulose Ethers and Bile Salts: Impact on In Vitro Lipid Digestion

Elevated levels of lipids and LDL-cholesterol in the blood are significant risk factors associated with developing cardiovascular diseases (CVDs). A potential strategy to combat these risk factors is decreasing lipid absorption by modulating the digestibility of lipids in the human intestinal tract. Since bile salts (BS) play key roles during this process, lipid digestion could be controlled ultimately by limiting the access of BS to the lipid surface. Cellulose ethers (CEs), surface-active dietary fibers and common food additives, might be promising ingredients to control lipid digestion either by creating surface layers around lipid droplets that hinder adsorption of BS, or by sequestering BS in the aqueous phase. However, the precise mechanisms behind these interactions remain unclear. Surface analysis techniques were used to better understand the mechanisms by which CEs with diverse molecular structure and charge (commercial and novel hydroxypropyl-cellulose (HPC)) interact with BS at the solid surface and in the aqueous phase. The potential of CE-stabilized emulsions to influence lipid digestion was also investigated in vitro. Both CEs show potential in modulating lipid digestion; the potential of the commercial HPC to interfere with lipid digestion may be more related to its ability to sequester BS in solution and form mixed HPC-BS complexes that are not easily removed from the surface, whereas the novel HPC seems more effective at creating strong surface layers that resist displacement by BS. These findings can be exploited in developing strategies to design novel food matrices with improved functional properties to optimize lipid digestion and absorption.

Name thesis:degree_name

MSLFS

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Food Science and Technology

Department dc:contributor.department

Food Science and Technology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

2019

Author dc:creator

• Zornjak, Jennifer Anne

Chair dc:contributor.committeechair

• Fernandez-Fraguas, Cristina

Committee members dc:contributor.committeemember

• Edgar, Kevin J.
• Neilson, Andrew P.
• Esker, Alan R.

• Hydroxypropyl Cellulose
• Bile Salts
• Lipid Digestion
• Quartz Crystal Microbalance with Dissipation Monitoring
• Cardiovascular Disease

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

vt_gsexam:18701

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/99285