A phylogenetic study of the genus Campylobacter

The relationships of the fourteen (14) species of Campylobacter were derived by comparison of the partial 16S ribosomal RNA sequences determined using reverse transcriptase and oligonucleotide primers specific for regions in the 16S rRNA molecule. These species formed three distinct RNA sequence homology groups. We propose that the following species remain in the genus Campylobacter (homology group I): C. coli, C. jejuni, C. laridis, C. fetus, C. hyointestinalis, C. concisus, C. mucosalis, C. sputorum and the "catalase-negative to weakly-positive" (CNW) strains. C. pylori, "C. cinaedi" and "C. fennelliae'' were not related to the true campylobacters at the genus level. However, they were related to each other and to Wolinella succinogenes at the genus level and constituted homology group II. These four species should be reclassified and placed within a single genus based on 16S rRNA sequence similarity. C. cryaerophila and C. nitrofigilis also exhibited a high level of RNA sequence homology with each other but not with any other species tested; they constituted homology group III and should be considered as being a single genus. A comparison of the 165 rRNA sequence data from the three homology groups with the same sequences from representatives of the three major phylogenetic branches of the purple bacteria indicated that these genera form a single phylogenetic branch which is only distantly related to the purple bacteria.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Microbiology

Department dc:contributor.department

Microbiology

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1987

Author dc:creator

• Thompson, Louis Milton

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/10919/80084

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/80084