{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/78309"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/78309","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Adsorption of Blood Proteins onto Polysaccharide Surfaces","abstract":"In this study, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) were combined to investigate the adsorption behavior of two platelet adhesion-related blood proteins, human serum albumin (HSA) and human serum fibrinogen (HSF), on two polysaccharide materials used for hemodialysis membrane applications: regenerated cellulose and cellulose acetate. The study aims to provide insight into the design of novel hemocompatible polysaccharide materials. Information such as real-time adsorption curves, adsorbed amounts, and water contents of the protein layers were obtained and analyzed. The results suggested 1) monolayer adsorption of HSA on both cellulose and cellulose acetate, possibly with different HSA conformations; 2) a multilayer of HSF or some degree of end-on adsorption on both surfaces. The study of HSA adsorption onto cellulose acetate surfaces with different degrees of substitution indicated that the change in content of acetyl groups may not be the main factor governing the adsorbed HSA amount but may affect the conformation of adsorbed HSA molecules.","abstract_html":"In this study, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) were combined to investigate the adsorption behavior of two platelet adhesion-related blood proteins, human serum albumin (HSA) and human serum fibrinogen (HSF), on two polysaccharide materials used for hemodialysis membrane applications: regenerated cellulose and cellulose acetate. The study aims to provide insight into the design of novel hemocompatible polysaccharide materials. Information such as real-time adsorption curves, adsorbed amounts, and water contents of the protein layers were obtained and analyzed. The results suggested 1) monolayer adsorption of HSA on both cellulose and cellulose acetate, possibly with different HSA conformations; 2) a multilayer of HSF or some degree of end-on adsorption on both surfaces. The study of HSA adsorption onto cellulose acetate surfaces with different degrees of substitution indicated that the change in content of acetyl groups may not be the main factor governing the adsorbed HSA amount but may affect the conformation of adsorbed HSA molecules.","abstract_has_math":false,"creators":["Tan, Xinyi"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Macromolecular Science and Engineering","degree_department":"Macromolecular Science and Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Roman, Maren"],"committee_members":["Edgar, Kevin J.","Esker, Alan R."],"year":2016,"date_issued":"2016-01-10","date_published":"2016-01-10","updated_at":"2026-07-22T22:20:38Z","subjects":["albumin","fibrinogen","cellulose acetate","cellulose","thin films","adsorption"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:6528"],"render_values":[{"text":"vt_gsexam:6528","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/78309","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Roman, Maren"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Edgar, Kevin J.","Esker, Alan R."]},{"key":"dc:contributor.department","label":"Department","values":["Macromolecular Science and Engineering"]},{"key":"dc:creator","label":"Author","values":["Tan, Xinyi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-07-04T06:00:16Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-07-04T06:00:16Z"]},{"key":"dc:date.issued","label":"Date","values":["2016-01-10"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Macromolecular Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["albumin","fibrinogen","cellulose acetate","cellulose","thin films","adsorption"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:6528"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/78309"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In this study, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) were combined to investigate the adsorption behavior of two platelet adhesion-related blood proteins, human serum albumin (HSA) and human serum fibrinogen (HSF), on two polysaccharide materials used for hemodialysis membrane applications: regenerated cellulose and cellulose acetate. The study aims to provide insight into the design of novel hemocompatible polysaccharide materials. Information such as real-time adsorption curves, adsorbed amounts, and water contents of the protein layers were obtained and analyzed. The results suggested 1) monolayer adsorption of HSA on both cellulose and cellulose acetate, possibly with different HSA conformations; 2) a multilayer of HSF or some degree of end-on adsorption on both surfaces. The study of HSA adsorption onto cellulose acetate surfaces with different degrees of substitution indicated that the change in content of acetyl groups may not be the main factor governing the adsorbed HSA amount but may affect the conformation of adsorbed HSA molecules."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["Adsorption of Blood Proteins onto Polysaccharide Surfaces"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Roman, Maren"],"dc:contributor.committeemember":["Edgar, Kevin J.","Esker, Alan R."],"dc:contributor.department":["Macromolecular Science and Engineering"],"dc:creator":["Tan, Xinyi"],"dc:date.accessioned":["2017-07-04T06:00:16Z"],"dc:date.available":["2017-07-04T06:00:16Z"],"dc:date.issued":["2016-01-10"],"dc:description.abstract":["In this study, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) were combined to investigate the adsorption behavior of two platelet adhesion-related blood proteins, human serum albumin (HSA) and human serum fibrinogen (HSF), on two polysaccharide materials used for hemodialysis membrane applications: regenerated cellulose and cellulose acetate. The study aims to provide insight into the design of novel hemocompatible polysaccharide materials. Information such as real-time adsorption curves, adsorbed amounts, and water contents of the protein layers were obtained and analyzed. The results suggested 1) monolayer adsorption of HSA on both cellulose and cellulose acetate, possibly with different HSA conformations; 2) a multilayer of HSF or some degree of end-on adsorption on both surfaces. The study of HSA adsorption onto cellulose acetate surfaces with different degrees of substitution indicated that the change in content of acetyl groups may not be the main factor governing the adsorbed HSA amount but may affect the conformation of adsorbed HSA molecules."],"dc:description.degree":["Master of Science"],"dc:format.medium":["ETD"],"dc:identifier.other":["vt_gsexam:6528"],"dc:identifier.uri":["http://hdl.handle.net/10919/78309"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["albumin","fibrinogen","cellulose acetate","cellulose","thin films","adsorption"],"dc:title":["Adsorption of Blood Proteins onto Polysaccharide Surfaces"],"dc:type":["Thesis"],"thesis:degree_discipline":["Macromolecular Science and Engineering"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:38Z"}