{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/77769"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/77769","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Identification of soybean, Glycine max (L.) Merr., cytokinins","abstract":"Soybean, Glycine max (L.) Merr., fruits 10-25 mm long contained nine fractions having cytokinin-like activity detectable by the Amaranthus bioassay. These fractions were derived from purification of extracts from 15 g of immature fruits. The fractions were purified by polyvinylpyrrolidone (PVP), paper, Sephadex LH-20 and high performance liquid chromatography. The fractions separated on the basis of polarity into polar, moderately polar and relatively non-polar groups, compared to zeatin. The polar fraction was the single most bioactive fraction and the active substance was tentatively identified as a zeatin glucoside or zeatin riboside glucoside. The moderately polar fraction contained bioactive substances that co-eluted with zeatin and zeatin riboside in four chromatographic procedures. The relatively non-polar fraction contained two bioactive components, one chromatographically resembling 2iPA, and the second less polar. A conjugated cytokinin was released from the original aqueous phase by alkaline phosphatase hydrolysis. This substance co-eluted with zeatin riboside on Sephadex LH-20. Further purification and analysis by multiple ion monitoring GC-MS did not yield conclusive identification of the cytokinins. Based on bioassay, the biological estimates of cytokinin-like substances present in the extracts were below the detection limit of the GC-MS. Obtaining sufficiently large samples of soybean fruits at the proper stage of physiological interest complicated definitive structural identification based on the purification and identification techniques used.","abstract_html":"Soybean, Glycine max (L.) Merr., fruits 10-25 mm long contained nine fractions having cytokinin-like activity detectable by the Amaranthus bioassay. These fractions were derived from purification of extracts from 15 g of immature fruits. The fractions were purified by polyvinylpyrrolidone (PVP), paper, Sephadex LH-20 and high performance liquid chromatography. The fractions separated on the basis of polarity into polar, moderately polar and relatively non-polar groups, compared to zeatin. The polar fraction was the single most bioactive fraction and the active substance was tentatively identified as a zeatin glucoside or zeatin riboside glucoside. The moderately polar fraction contained bioactive substances that co-eluted with zeatin and zeatin riboside in four chromatographic procedures. The relatively non-polar fraction contained two bioactive components, one chromatographically resembling 2iPA, and the second less polar. A conjugated cytokinin was released from the original aqueous phase by alkaline phosphatase hydrolysis. This substance co-eluted with zeatin riboside on Sephadex LH-20. Further purification and analysis by multiple ion monitoring GC-MS did not yield conclusive identification of the cytokinins. Based on bioassay, the biological estimates of cytokinin-like substances present in the extracts were below the detection limit of the GC-MS. Obtaining sufficiently large samples of soybean fruits at the proper stage of physiological interest complicated definitive structural identification based on the purification and identification techniques used.","abstract_has_math":false,"creators":["Crosby, Kevin Edward"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Agronomy","degree_department":"Agronomy","school":null,"contributors":[],"advisors":[],"committee_chairs":["Aung, L.H.","Buss, G.R."],"committee_members":["Hess, John L.","Kingston, David G. I.","Stout, Ernest R."],"year":1982,"date_issued":"1982","date_published":"1982","updated_at":"2026-07-22T22:19:12Z","subjects":[],"languages":["en_US"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/77769","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Aung, L.H.","Buss, G.R."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Hess, John L.","Kingston, David G. 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The fractions were purified by polyvinylpyrrolidone (PVP), paper, Sephadex LH-20 and high performance liquid chromatography. The fractions separated on the basis of polarity into polar, moderately polar and relatively non-polar groups, compared to zeatin. The polar fraction was the single most bioactive fraction and the active substance was tentatively identified as a zeatin glucoside or zeatin riboside glucoside. The moderately polar fraction contained bioactive substances that co-eluted with zeatin and zeatin riboside in four chromatographic procedures. The relatively non-polar fraction contained two bioactive components, one chromatographically resembling 2iPA, and the second less polar. A conjugated cytokinin was released from the original aqueous phase by alkaline phosphatase hydrolysis. This substance co-eluted with zeatin riboside on Sephadex LH-20. Further purification and analysis by multiple ion monitoring GC-MS did not yield conclusive identification of the cytokinins. Based on bioassay, the biological estimates of cytokinin-like substances present in the extracts were below the detection limit of the GC-MS. Obtaining sufficiently large samples of soybean fruits at the proper stage of physiological interest complicated definitive structural identification based on the purification and identification techniques used."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Identification of soybean, Glycine max (L.) Merr., cytokinins"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Aung, L.H.","Buss, G.R."],"dc:contributor.committeemember":["Hess, John L.","Kingston, David G. 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The moderately polar fraction contained bioactive substances that co-eluted with zeatin and zeatin riboside in four chromatographic procedures. The relatively non-polar fraction contained two bioactive components, one chromatographically resembling 2iPA, and the second less polar. A conjugated cytokinin was released from the original aqueous phase by alkaline phosphatase hydrolysis. This substance co-eluted with zeatin riboside on Sephadex LH-20. Further purification and analysis by multiple ion monitoring GC-MS did not yield conclusive identification of the cytokinins. Based on bioassay, the biological estimates of cytokinin-like substances present in the extracts were below the detection limit of the GC-MS. Obtaining sufficiently large samples of soybean fruits at the proper stage of physiological interest complicated definitive structural identification based on the purification and identification techniques used."],"dc:description.degree":["Ph. D."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/77769"],"dc:language.iso":["en_US"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Identification of soybean, Glycine max (L.) Merr., cytokinins"],"dc:type":["Dissertation"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Agronomy"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Ph. D."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:12Z"}