{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/76584"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/76584","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Long-term storage of liquid boar spermatozoa","abstract":"This study was conducted to: (a) determine the optimum extender system for two extenders that will maintain the highest level of cellular integrity when stored at either 5C or 15C for a minimum of 72 hr; (b) evaluate the fertilizing capacity of stored spermatozoa using the optimum extender system; (c) critically analyze enzymatic and morphological changes associated with storage and aging of boar spermatozoa; and (d) characterize properties of boar spermatozoa important to fertilization. The least-squares means for the percentage of normal apical ridge acrosomes were significantly affected by ejaculate within boar (P< .001), boar x extender (P< .05), boar x storage temperature (P< .01), extender x storage temperature (P<.01), extender x cooling rate (P< .05) and antibiotic x storage temperature (P< .01). The regression equations of storage time x treatment effect were reported. The least-squares means for acrosin activity were significantly affected by ejaculate within boar (P<.001), ejaculate within boar x antibiotic (P< .05), ejaculate within boar x extender (P<. 001), ejaculate within boar x storage temperature and extender x storage temperature (P< .05). The regression of storage time x storage temperature was reported. The least-squares means for progressive forward motility were significantly affected by extender (P< .05), antibiotic (P< .05), ejaculate within boar (P< .001), antibiotic x extender (P< .05) and extender x storage temperature (P< .01). The regressions of storage time x treatment effects were reported. The least-squares means for vibrational and/or rotational motility were affected by ejaculate within boar (P< .001), ejaculate within boar x extender (P<.001), ejaculate within boar x cooling rate (P<.05), antibiotic x extender and extender x storage temperature (P< .01). The optimum extender system consisted of Purdue extender containing penicillin/streptomycin and cooled to a 15C storage temperature in 4 hours. This extender system maintained a 70% minimum fertilization rate for 84 hours.","abstract_html":"This study was conducted to: (a) determine the optimum extender system for two extenders that will maintain the highest level of cellular integrity when stored at either 5C or 15C for a minimum of 72 hr; (b) evaluate the fertilizing capacity of stored spermatozoa using the optimum extender system; (c) critically analyze enzymatic and morphological changes associated with storage and aging of boar spermatozoa; and (d) characterize properties of boar spermatozoa important to fertilization. The least-squares means for the percentage of normal apical ridge acrosomes were significantly affected by ejaculate within boar (P&lt; .001), boar x extender (P&lt; .05), boar x storage temperature (P&lt; .01), extender x storage temperature (P&lt;.01), extender x cooling rate (P&lt; .05) and antibiotic x storage temperature (P&lt; .01). The regression equations of storage time x treatment effect were reported. The least-squares means for acrosin activity were significantly affected by ejaculate within boar (P&lt;.001), ejaculate within boar x antibiotic (P&lt; .05), ejaculate within boar x extender (P&lt;. 001), ejaculate within boar x storage temperature and extender x storage temperature (P&lt; .05). The regression of storage time x storage temperature was reported. The least-squares means for progressive forward motility were significantly affected by extender (P&lt; .05), antibiotic (P&lt; .05), ejaculate within boar (P&lt; .001), antibiotic x extender (P&lt; .05) and extender x storage temperature (P&lt; .01). The regressions of storage time x treatment effects were reported. The least-squares means for vibrational and/or rotational motility were affected by ejaculate within boar (P&lt; .001), ejaculate within boar x extender (P&lt;.001), ejaculate within boar x cooling rate (P&lt;.05), antibiotic x extender and extender x storage temperature (P&lt; .01). The optimum extender system consisted of Purdue extender containing penicillin/streptomycin and cooled to a 15C storage temperature in 4 hours. This extender system maintained a 70% minimum fertilization rate for 84 hours.","abstract_has_math":false,"creators":["Underwood, Charles Raymond"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Animal Science (Reproductive Physiology)","degree_department":"Animal Science (Reproductive Physiology)","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1981,"date_issued":"1981","date_published":"1981","updated_at":"2026-07-22T22:20:40Z","subjects":[],"languages":["en_US"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/76584","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Animal Science (Reproductive Physiology)"]},{"key":"dc:creator","label":"Author","values":["Underwood, Charles Raymond"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-03-10T21:56:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-03-10T21:56:00Z"]},{"key":"dc:date.issued","label":"Date","values":["1981"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Animal Science (Reproductive Physiology)"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph. 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The least-squares means for the percentage of normal apical ridge acrosomes were significantly affected by ejaculate within boar (P< .001), boar x extender (P< .05), boar x storage temperature (P< .01), extender x storage temperature (P<.01), extender x cooling rate (P< .05) and antibiotic x storage temperature (P< .01). The regression equations of storage time x treatment effect were reported. The least-squares means for acrosin activity were significantly affected by ejaculate within boar (P<.001), ejaculate within boar x antibiotic (P< .05), ejaculate within boar x extender (P<. 001), ejaculate within boar x storage temperature and extender x storage temperature (P< .05). The regression of storage time x storage temperature was reported. The least-squares means for progressive forward motility were significantly affected by extender (P< .05), antibiotic (P< .05), ejaculate within boar (P< .001), antibiotic x extender (P< .05) and extender x storage temperature (P< .01). The regressions of storage time x treatment effects were reported. The least-squares means for vibrational and/or rotational motility were affected by ejaculate within boar (P< .001), ejaculate within boar x extender (P<.001), ejaculate within boar x cooling rate (P<.05), antibiotic x extender and extender x storage temperature (P< .01). The optimum extender system consisted of Purdue extender containing penicillin/streptomycin and cooled to a 15C storage temperature in 4 hours. This extender system maintained a 70% minimum fertilization rate for 84 hours."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Long-term storage of liquid boar spermatozoa"]}]}],"canonical_facts":{"dc:contributor.department":["Animal Science (Reproductive Physiology)"],"dc:creator":["Underwood, Charles Raymond"],"dc:date.accessioned":["2017-03-10T21:56:00Z"],"dc:date.available":["2017-03-10T21:56:00Z"],"dc:date.issued":["1981"],"dc:description.abstract":["This study was conducted to: (a) determine the optimum extender system for two extenders that will maintain the highest level of cellular integrity when stored at either 5C or 15C for a minimum of 72 hr; (b) evaluate the fertilizing capacity of stored spermatozoa using the optimum extender system; (c) critically analyze enzymatic and morphological changes associated with storage and aging of boar spermatozoa; and (d) characterize properties of boar spermatozoa important to fertilization. The least-squares means for the percentage of normal apical ridge acrosomes were significantly affected by ejaculate within boar (P< .001), boar x extender (P< .05), boar x storage temperature (P< .01), extender x storage temperature (P<.01), extender x cooling rate (P< .05) and antibiotic x storage temperature (P< .01). The regression equations of storage time x treatment effect were reported. The least-squares means for acrosin activity were significantly affected by ejaculate within boar (P<.001), ejaculate within boar x antibiotic (P< .05), ejaculate within boar x extender (P<. 001), ejaculate within boar x storage temperature and extender x storage temperature (P< .05). The regression of storage time x storage temperature was reported. The least-squares means for progressive forward motility were significantly affected by extender (P< .05), antibiotic (P< .05), ejaculate within boar (P< .001), antibiotic x extender (P< .05) and extender x storage temperature (P< .01). The regressions of storage time x treatment effects were reported. The least-squares means for vibrational and/or rotational motility were affected by ejaculate within boar (P< .001), ejaculate within boar x extender (P<.001), ejaculate within boar x cooling rate (P<.05), antibiotic x extender and extender x storage temperature (P< .01). The optimum extender system consisted of Purdue extender containing penicillin/streptomycin and cooled to a 15C storage temperature in 4 hours. This extender system maintained a 70% minimum fertilization rate for 84 hours."],"dc:description.degree":["Ph. D."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/76584"],"dc:language.iso":["en_US"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Long-term storage of liquid boar spermatozoa"],"dc:type":["Dissertation"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Animal Science (Reproductive Physiology)"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Ph. D."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:40Z"}