{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/71084"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/71084","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The isolation and partial characterization of rat kidney stone matrix induced by magnesium deficiency","abstract":"Dietary magnesium deficiency in rats has been employed to generate kidney stones in a relatively short period of time. Stones have been observed in situ and examined histochemically. Techniques have been devised for the isolation of the kidney stones and for the demineralization of the stones. The gross morphological character of isolated concretions has been examined microscopically, and the mineral composition determined by X-ray emission analysis. Hydroxyapatite was found to be the mineral component. Decalcification and subsequent dialysis of stones revealed that about 60% of the stone, on a dry weight basis, consists of apatite, and about. 5.8% consists of a nondialyzable organic component termed matrix. A variety of chemical analyses were done on this matrix material. These tests included elemental analysis, amino acid analysis, a variety of carbohydrate assays, fatty acid analysis, a cholesterol determination, phosphate analysis, and measurements of calcium and magnesium content. From these studies, about 60% of the matrix composition has been determined, the majority of which are amino acids and neutral carbohydrate. The origin and function of kidney stone matrix are unknown. Possible answers to these questions have been presented, although insufficient data does not permit any conclusive statement to be made. Knowledge of.the composition of matrix may be useful in determining its origin.","abstract_html":"Dietary magnesium deficiency in rats has been employed to generate kidney stones in a relatively short period of time. Stones have been observed in situ and examined histochemically. Techniques have been devised for the isolation of the kidney stones and for the demineralization of the stones. The gross morphological character of isolated concretions has been examined microscopically, and the mineral composition determined by X-ray emission analysis. Hydroxyapatite was found to be the mineral component. Decalcification and subsequent dialysis of stones revealed that about 60% of the stone, on a dry weight basis, consists of apatite, and about. 5.8% consists of a nondialyzable organic component termed matrix. A variety of chemical analyses were done on this matrix material. These tests included elemental analysis, amino acid analysis, a variety of carbohydrate assays, fatty acid analysis, a cholesterol determination, phosphate analysis, and measurements of calcium and magnesium content. From these studies, about 60% of the matrix composition has been determined, the majority of which are amino acids and neutral carbohydrate. The origin and function of kidney stone matrix are unknown. Possible answers to these questions have been presented, although insufficient data does not permit any conclusive statement to be made. Knowledge of.the composition of matrix may be useful in determining its origin.","abstract_has_math":false,"creators":["King, Gregory Allen"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Biochemistry and Nutrition","degree_department":"Biochemistry and Nutrition","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1976,"date_issued":"1976","date_published":"1976","updated_at":"2026-07-22T22:19:03Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/71084","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Biochemistry and Nutrition"]},{"key":"dc:creator","label":"Author","values":["King, Gregory Allen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-05-23T14:57:49Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-05-23T14:57:49Z"]},{"key":"dc:date.issued","label":"Date","values":["1976"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry and Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/71084"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Dietary magnesium deficiency in rats has been employed to generate kidney stones in a relatively short period of time. Stones have been observed in situ and examined histochemically. Techniques have been devised for the isolation of the kidney stones and for the demineralization of the stones. The gross morphological character of isolated concretions has been examined microscopically, and the mineral composition determined by X-ray emission analysis. Hydroxyapatite was found to be the mineral component. Decalcification and subsequent dialysis of stones revealed that about 60% of the stone, on a dry weight basis, consists of apatite, and about. 5.8% consists of a nondialyzable organic component termed matrix. A variety of chemical analyses were done on this matrix material. These tests included elemental analysis, amino acid analysis, a variety of carbohydrate assays, fatty acid analysis, a cholesterol determination, phosphate analysis, and measurements of calcium and magnesium content. From these studies, about 60% of the matrix composition has been determined, the majority of which are amino acids and neutral carbohydrate. The origin and function of kidney stone matrix are unknown. Possible answers to these questions have been presented, although insufficient data does not permit any conclusive statement to be made. Knowledge of.the composition of matrix may be useful in determining its origin."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The isolation and partial characterization of rat kidney stone matrix induced by magnesium deficiency"]}]}],"canonical_facts":{"dc:contributor.department":["Biochemistry and Nutrition"],"dc:creator":["King, Gregory Allen"],"dc:date.accessioned":["2016-05-23T14:57:49Z"],"dc:date.available":["2016-05-23T14:57:49Z"],"dc:date.issued":["1976"],"dc:description.abstract":["Dietary magnesium deficiency in rats has been employed to generate kidney stones in a relatively short period of time. Stones have been observed in situ and examined histochemically. Techniques have been devised for the isolation of the kidney stones and for the demineralization of the stones. The gross morphological character of isolated concretions has been examined microscopically, and the mineral composition determined by X-ray emission analysis. Hydroxyapatite was found to be the mineral component. Decalcification and subsequent dialysis of stones revealed that about 60% of the stone, on a dry weight basis, consists of apatite, and about. 5.8% consists of a nondialyzable organic component termed matrix. A variety of chemical analyses were done on this matrix material. These tests included elemental analysis, amino acid analysis, a variety of carbohydrate assays, fatty acid analysis, a cholesterol determination, phosphate analysis, and measurements of calcium and magnesium content. From these studies, about 60% of the matrix composition has been determined, the majority of which are amino acids and neutral carbohydrate. The origin and function of kidney stone matrix are unknown. Possible answers to these questions have been presented, although insufficient data does not permit any conclusive statement to be made. Knowledge of.the composition of matrix may be useful in determining its origin."],"dc:description.degree":["Master of Science"],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/71084"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["The isolation and partial characterization of rat kidney stone matrix induced by magnesium deficiency"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Biochemistry and Nutrition"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:03Z"}