{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/70491"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/70491","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The effects of the three soil-inhabiting fungi on peanuts grown under germ-free conditions","abstract":"Excised peanut embryos were sterilized by treatment with 1% NaOCl tor 5 minutes, and placed in 250-ml flasks containing nutrients and expanded granular shale. Nutrients consisted of Hoagland's solution supplemented with 1% sucrose and 0.03 ppm gibberellic acid. Plants were grown for 4 weeks in flasks placed between tubes of fluorescent lights at room temperature. When the plants were 2 to 3 inches tall the flasks were inoculated in the root zone singly and in all combinations with Aspergillus flavus, Streptomyces griseus, and Trichoderma sp. All 3 organisms invaded the root tissue. When compared with uninoculated check plants, S. griseus individually caused no measurable damage, but in combination caused stunting and chlorosis. A flavus and Trichoderma sp. alone killed plants within 15 and 20 days, respectively, after inoculation; while in combination they killed plants within 15 days. Inoculations of plants with all 3 fungi in combination caused less damage.","abstract_html":"Excised peanut embryos were sterilized by treatment with 1% NaOCl tor 5 minutes, and placed in 250-ml flasks containing nutrients and expanded granular shale. Nutrients consisted of Hoagland&#x27;s solution supplemented with 1% sucrose and 0.03 ppm gibberellic acid. Plants were grown for 4 weeks in flasks placed between tubes of fluorescent lights at room temperature. When the plants were 2 to 3 inches tall the flasks were inoculated in the root zone singly and in all combinations with Aspergillus flavus, Streptomyces griseus, and Trichoderma sp. All 3 organisms invaded the root tissue. When compared with uninoculated check plants, S. griseus individually caused no measurable damage, but in combination caused stunting and chlorosis. A flavus and Trichoderma sp. alone killed plants within 15 and 20 days, respectively, after inoculation; while in combination they killed plants within 15 days. Inoculations of plants with all 3 fungi in combination caused less damage.","abstract_has_math":false,"creators":["Chang, Chen-hong Ting"],"institution":"Virginia Polytechnic Institute","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Plant Pathology","degree_department":"Plant Pathology","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1967,"date_issued":"1967","date_published":"1967","updated_at":"2026-07-22T22:20:43Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/70491","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Plant Pathology"]},{"key":"dc:creator","label":"Author","values":["Chang, Chen-hong Ting"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-04-21T15:34:47Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-04-21T15:34:47Z"]},{"key":"dc:date.issued","label":"Date","values":["1967"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/70491"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Excised peanut embryos were sterilized by treatment with 1% NaOCl tor 5 minutes, and placed in 250-ml flasks containing nutrients and expanded granular shale. Nutrients consisted of Hoagland's solution supplemented with 1% sucrose and 0.03 ppm gibberellic acid. Plants were grown for 4 weeks in flasks placed between tubes of fluorescent lights at room temperature. When the plants were 2 to 3 inches tall the flasks were inoculated in the root zone singly and in all combinations with Aspergillus flavus, Streptomyces griseus, and Trichoderma sp. All 3 organisms invaded the root tissue. When compared with uninoculated check plants, S. griseus individually caused no measurable damage, but in combination caused stunting and chlorosis. A flavus and Trichoderma sp. alone killed plants within 15 and 20 days, respectively, after inoculation; while in combination they killed plants within 15 days. Inoculations of plants with all 3 fungi in combination caused less damage."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The effects of the three soil-inhabiting fungi on peanuts grown under germ-free conditions"]}]}],"canonical_facts":{"dc:contributor.department":["Plant Pathology"],"dc:creator":["Chang, Chen-hong Ting"],"dc:date.accessioned":["2016-04-21T15:34:47Z"],"dc:date.available":["2016-04-21T15:34:47Z"],"dc:date.issued":["1967"],"dc:description.abstract":["Excised peanut embryos were sterilized by treatment with 1% NaOCl tor 5 minutes, and placed in 250-ml flasks containing nutrients and expanded granular shale. Nutrients consisted of Hoagland's solution supplemented with 1% sucrose and 0.03 ppm gibberellic acid. Plants were grown for 4 weeks in flasks placed between tubes of fluorescent lights at room temperature. When the plants were 2 to 3 inches tall the flasks were inoculated in the root zone singly and in all combinations with Aspergillus flavus, Streptomyces griseus, and Trichoderma sp. All 3 organisms invaded the root tissue. When compared with uninoculated check plants, S. griseus individually caused no measurable damage, but in combination caused stunting and chlorosis. A flavus and Trichoderma sp. alone killed plants within 15 and 20 days, respectively, after inoculation; while in combination they killed plants within 15 days. Inoculations of plants with all 3 fungi in combination caused less damage."],"dc:description.degree":["Master of Science"],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/70491"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["The effects of the three soil-inhabiting fungi on peanuts grown under germ-free conditions"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Plant Pathology"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute"]},"updated_at":"2026-07-22T22:20:43Z"}