{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/64100"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/64100","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Derivation and chromatographic separation of acid indole auxins","abstract":"For many years silylation of polar and thermolabile compounds has been used to facilitate their gas chromatographic analysis. The three phytochemically significant indole acids; 3-indole acetic acid, 3-indole propionic acid, and 3-indole butyric acid along with an analog, D-tryptophan, are four compounds which have been analyzed by this method. A detailed study of the trimethylsilylation reaction of these compounds was the objective of this research. Optimum conditions for the trimethylsilylation of the four acid indoles, including temperature, time of silylation, humidity and reagent excess were examined. Practical considerations for the gas chromatographic separation of these compounds, including column length, choice of liquid phase, and column temperature were studied and optimized. The effects of derivative storage were examined and optimum storage conditions, alone; with the stability of the primary reaction product, were determined. The structure of reaction side products and decomposition products were thoroughly investigated. To this end an all glass post column effluent splitter was designed and installed for the trapping of derivatives. Finally, the feasibility of using high speed liquid chromatography for the separation and analysis of these four compounds was also investigated.","abstract_html":"For many years silylation of polar and thermolabile compounds has been used to facilitate their gas chromatographic analysis. The three phytochemically significant indole acids; 3-indole acetic acid, 3-indole propionic acid, and 3-indole butyric acid along with an analog, D-tryptophan, are four compounds which have been analyzed by this method. A detailed study of the trimethylsilylation reaction of these compounds was the objective of this research. Optimum conditions for the trimethylsilylation of the four acid indoles, including temperature, time of silylation, humidity and reagent excess were examined. Practical considerations for the gas chromatographic separation of these compounds, including column length, choice of liquid phase, and column temperature were studied and optimized. The effects of derivative storage were examined and optimum storage conditions, alone; with the stability of the primary reaction product, were determined. The structure of reaction side products and decomposition products were thoroughly investigated. To this end an all glass post column effluent splitter was designed and installed for the trapping of derivatives. Finally, the feasibility of using high speed liquid chromatography for the separation and analysis of these four compounds was also investigated.","abstract_has_math":false,"creators":["Marsh, Daniel Griggs"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Chemistry","degree_department":"Chemistry","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1974,"date_issued":"1974","date_published":"1974","updated_at":"2026-07-22T22:20:34Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/64100","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Chemistry"]},{"key":"dc:creator","label":"Author","values":["Marsh, Daniel Griggs"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-11-13T20:44:37Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-11-13T20:44:37Z"]},{"key":"dc:date.issued","label":"Date","values":["1974"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/64100"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["For many years silylation of polar and thermolabile compounds has been used to facilitate their gas chromatographic analysis. The three phytochemically significant indole acids; 3-indole acetic acid, 3-indole propionic acid, and 3-indole butyric acid along with an analog, D-tryptophan, are four compounds which have been analyzed by this method. A detailed study of the trimethylsilylation reaction of these compounds was the objective of this research. Optimum conditions for the trimethylsilylation of the four acid indoles, including temperature, time of silylation, humidity and reagent excess were examined. Practical considerations for the gas chromatographic separation of these compounds, including column length, choice of liquid phase, and column temperature were studied and optimized. The effects of derivative storage were examined and optimum storage conditions, alone; with the stability of the primary reaction product, were determined. The structure of reaction side products and decomposition products were thoroughly investigated. To this end an all glass post column effluent splitter was designed and installed for the trapping of derivatives. Finally, the feasibility of using high speed liquid chromatography for the separation and analysis of these four compounds was also investigated."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Derivation and chromatographic separation of acid indole auxins"]}]}],"canonical_facts":{"dc:contributor.department":["Chemistry"],"dc:creator":["Marsh, Daniel Griggs"],"dc:date.accessioned":["2015-11-13T20:44:37Z"],"dc:date.available":["2015-11-13T20:44:37Z"],"dc:date.issued":["1974"],"dc:description.abstract":["For many years silylation of polar and thermolabile compounds has been used to facilitate their gas chromatographic analysis. The three phytochemically significant indole acids; 3-indole acetic acid, 3-indole propionic acid, and 3-indole butyric acid along with an analog, D-tryptophan, are four compounds which have been analyzed by this method. A detailed study of the trimethylsilylation reaction of these compounds was the objective of this research. Optimum conditions for the trimethylsilylation of the four acid indoles, including temperature, time of silylation, humidity and reagent excess were examined. Practical considerations for the gas chromatographic separation of these compounds, including column length, choice of liquid phase, and column temperature were studied and optimized. The effects of derivative storage were examined and optimum storage conditions, alone; with the stability of the primary reaction product, were determined. The structure of reaction side products and decomposition products were thoroughly investigated. To this end an all glass post column effluent splitter was designed and installed for the trapping of derivatives. Finally, the feasibility of using high speed liquid chromatography for the separation and analysis of these four compounds was also investigated."],"dc:description.degree":["Master of Science"],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/64100"],"dc:language.iso":["en"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Derivation and chromatographic separation of acid indole auxins"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:34Z"}