{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/56173"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/56173","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Studies on the uptake and fate of hemoglobin pinocytosed by rat embryo fibroblasts cultured in normal or low magnesium media","abstract":"Rat embryo fibroblasts were used to study the metabolism of FlHb in normal and low Mg²⁺ media. The fibroblasts were cultured in a modified Eagle's basal medium with a 10% calf serum supplement. The test substrate was in a concentration of 200 μg/ml. Accumulation and spike experiments were conducted to measure uptake and digestion. Turnover experiments measured rates of digestion of a pinocytosed substrate. The accumulation was between 7 and 15 x 10⁻⁸ mmoles/mg cell protein. The half life for ingested FlHb was about 24 hours. There was considerable variation in accumulation per weight of cell protein when compared to the quantity of protein present. The lower the protein, the higher the accumulation. From dose dependent experiments there appeared to be surface binding saturation. The turnover rate was approximately 5% of the FlHb per day. There was no difference between the metabolism of FlHb by fibroblasts in the low Mg²⁺ or normal media.","abstract_html":"Rat embryo fibroblasts were used to study the metabolism of FlHb in normal and low Mg²⁺ media. The fibroblasts were cultured in a modified Eagle's basal medium with a 10% calf serum supplement. The test substrate was in a concentration of 200 μg/ml. Accumulation and spike experiments were conducted to measure uptake and digestion. Turnover experiments measured rates of digestion of a pinocytosed substrate. The accumulation was between 7 and 15 x 10⁻⁸ mmoles/mg cell protein. The half life for ingested FlHb was about 24 hours. There was considerable variation in accumulation per weight of cell protein when compared to the quantity of protein present. The lower the protein, the higher the accumulation. From dose dependent experiments there appeared to be surface binding saturation. The turnover rate was approximately 5% of the FlHb per day. There was no difference between the metabolism of FlHb by fibroblasts in the low Mg²⁺ or normal media.","abstract_has_math":false,"creators":["Bradford, William Wills"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Biochemistry and Nutrition","degree_department":"Biochemistry and Nutrition","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1973,"date_issued":"1973","date_published":"1973","updated_at":"2026-07-22T22:19:49Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/56173","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Biochemistry and Nutrition"]},{"key":"dc:creator","label":"Author","values":["Bradford, William Wills"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-08-07T14:18:04Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-08-07T14:18:04Z"]},{"key":"dc:date.issued","label":"Date","values":["1973"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry and Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/56173"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Rat embryo fibroblasts were used to study the metabolism of FlHb in normal and low Mg²⁺ media. The fibroblasts were cultured in a modified Eagle's basal medium with a 10% calf serum supplement. The test substrate was in a concentration of 200 μg/ml. Accumulation and spike experiments were conducted to measure uptake and digestion. Turnover experiments measured rates of digestion of a pinocytosed substrate. The accumulation was between 7 and 15 x 10⁻⁸ mmoles/mg cell protein. The half life for ingested FlHb was about 24 hours. There was considerable variation in accumulation per weight of cell protein when compared to the quantity of protein present. The lower the protein, the higher the accumulation. From dose dependent experiments there appeared to be surface binding saturation. The turnover rate was approximately 5% of the FlHb per day. There was no difference between the metabolism of FlHb by fibroblasts in the low Mg²⁺ or normal media."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Studies on the uptake and fate of hemoglobin pinocytosed by rat embryo fibroblasts cultured in normal or low magnesium media"]}]}],"canonical_facts":{"dc:contributor.department":["Biochemistry and Nutrition"],"dc:creator":["Bradford, William Wills"],"dc:date.accessioned":["2015-08-07T14:18:04Z"],"dc:date.available":["2015-08-07T14:18:04Z"],"dc:date.issued":["1973"],"dc:description.abstract":["Rat embryo fibroblasts were used to study the metabolism of FlHb in normal and low Mg²⁺ media. The fibroblasts were cultured in a modified Eagle's basal medium with a 10% calf serum supplement. The test substrate was in a concentration of 200 μg/ml. Accumulation and spike experiments were conducted to measure uptake and digestion. Turnover experiments measured rates of digestion of a pinocytosed substrate. The accumulation was between 7 and 15 x 10⁻⁸ mmoles/mg cell protein. The half life for ingested FlHb was about 24 hours. There was considerable variation in accumulation per weight of cell protein when compared to the quantity of protein present. The lower the protein, the higher the accumulation. From dose dependent experiments there appeared to be surface binding saturation. The turnover rate was approximately 5% of the FlHb per day. 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