{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/53934"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/53934","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Separation and quantitation of the seven forms of vitamin B-6 in plasma and 4-pyridoxic acid in urine of adolescent girls by reverse phase high performance liquid chromatography","abstract":"The vitamin B-6 status of seemingly healthy adolescent girls was determined using several accepted and proposed parameters in an effort to establish guidelines for status evaluation. HPLC-derived plasma B-6 vitamer [pyridoxal phosphate (PLP), pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM)] and 4-pyridoxic acid (4-PA) concentrations and urinary 4-PA levels of 28 white adolescent females, 12-15 years, having radiomonitored plasma PLP concentrations and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate status were determined. Mean daily vitamin B-6 and protein intakes of the subjects were 1.48 mg and 78.3 g, respectively. The ranges for plasma B-6 vitamer and 4-PA concentrations for these subjects which had seemingly adequate vitamin B-6 status were as follows: (nmol/L) PLP, 40.9-122.2; PNP, 0-16.1; PMP, 0-8.1; PL, 0- 15.0; PN, 0-21.9; PM, 0-17.8; and 4-PA, 0-55.7. PLP was the predominant plasma B-6 vitamer as well as being the only vitamer found in plasma of all subjects. Urinary 4-PA concentrations of the girls ranged from 0.11-2.50 pmol/mmol creatinine. The B-6 vitamer values of these white adolescent girls should be of use in the establishment of normal ranges for vitamin B-6 status parameters. HPLC methodologies seem to be advantageous for the rapid and accurate assessment of vitamin B-6 status.","abstract_html":"The vitamin B-6 status of seemingly healthy adolescent girls was determined using several accepted and proposed parameters in an effort to establish guidelines for status evaluation. HPLC-derived plasma B-6 vitamer [pyridoxal phosphate (PLP), pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM)] and 4-pyridoxic acid (4-PA) concentrations and urinary 4-PA levels of 28 white adolescent females, 12-15 years, having radiomonitored plasma PLP concentrations and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate status were determined. Mean daily vitamin B-6 and protein intakes of the subjects were 1.48 mg and 78.3 g, respectively. The ranges for plasma B-6 vitamer and 4-PA concentrations for these subjects which had seemingly adequate vitamin B-6 status were as follows: (nmol/L) PLP, 40.9-122.2; PNP, 0-16.1; PMP, 0-8.1; PL, 0- 15.0; PN, 0-21.9; PM, 0-17.8; and 4-PA, 0-55.7. PLP was the predominant plasma B-6 vitamer as well as being the only vitamer found in plasma of all subjects. Urinary 4-PA concentrations of the girls ranged from 0.11-2.50 pmol/mmol creatinine. The B-6 vitamer values of these white adolescent girls should be of use in the establishment of normal ranges for vitamin B-6 status parameters. HPLC methodologies seem to be advantageous for the rapid and accurate assessment of vitamin B-6 status.","abstract_has_math":false,"creators":["Chrisley, Barbara Mc"],"institution":"Virginia Polytechnic Institute and State University","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Human Nutrition and Foods","degree_department":"Human Nutrition and Foods","school":null,"contributors":[],"advisors":[],"committee_chairs":["Driskell, Judy A."],"committee_members":["Ritchey, Sanford J.","McNair, Harold M.","Johnson, J.M.","Young, R.W."],"year":1988,"date_issued":"1988","date_published":"1988","updated_at":"2026-07-22T22:20:26Z","subjects":[],"languages":["en_US"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/10919/53934","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Driskell, Judy A."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Ritchey, Sanford J.","McNair, Harold M.","Johnson, J.M.","Young, R.W."]},{"key":"dc:contributor.department","label":"Department","values":["Human Nutrition and Foods"]},{"key":"dc:creator","label":"Author","values":["Chrisley, Barbara Mc"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-06-29T22:07:11Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-06-29T22:07:11Z"]},{"key":"dc:date.issued","label":"Date","values":["1988"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Polytechnic Institute and State University"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Human Nutrition and Foods"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph. D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/53934"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The vitamin B-6 status of seemingly healthy adolescent girls was determined using several accepted and proposed parameters in an effort to establish guidelines for status evaluation. HPLC-derived plasma B-6 vitamer [pyridoxal phosphate (PLP), pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM)] and 4-pyridoxic acid (4-PA) concentrations and urinary 4-PA levels of 28 white adolescent females, 12-15 years, having radiomonitored plasma PLP concentrations and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate status were determined. Mean daily vitamin B-6 and protein intakes of the subjects were 1.48 mg and 78.3 g, respectively. The ranges for plasma B-6 vitamer and 4-PA concentrations for these subjects which had seemingly adequate vitamin B-6 status were as follows: (nmol/L) PLP, 40.9-122.2; PNP, 0-16.1; PMP, 0-8.1; PL, 0- 15.0; PN, 0-21.9; PM, 0-17.8; and 4-PA, 0-55.7. PLP was the predominant plasma B-6 vitamer as well as being the only vitamer found in plasma of all subjects. Urinary 4-PA concentrations of the girls ranged from 0.11-2.50 pmol/mmol creatinine. The B-6 vitamer values of these white adolescent girls should be of use in the establishment of normal ranges for vitamin B-6 status parameters. HPLC methodologies seem to be advantageous for the rapid and accurate assessment of vitamin B-6 status."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Separation and quantitation of the seven forms of vitamin B-6 in plasma and 4-pyridoxic acid in urine of adolescent girls by reverse phase high performance liquid chromatography"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Driskell, Judy A."],"dc:contributor.committeemember":["Ritchey, Sanford J.","McNair, Harold M.","Johnson, J.M.","Young, R.W."],"dc:contributor.department":["Human Nutrition and Foods"],"dc:creator":["Chrisley, Barbara Mc"],"dc:date.accessioned":["2015-06-29T22:07:11Z"],"dc:date.available":["2015-06-29T22:07:11Z"],"dc:date.issued":["1988"],"dc:description.abstract":["The vitamin B-6 status of seemingly healthy adolescent girls was determined using several accepted and proposed parameters in an effort to establish guidelines for status evaluation. HPLC-derived plasma B-6 vitamer [pyridoxal phosphate (PLP), pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), pyridoxal (PL), pyridoxine (PN), and pyridoxamine (PM)] and 4-pyridoxic acid (4-PA) concentrations and urinary 4-PA levels of 28 white adolescent females, 12-15 years, having radiomonitored plasma PLP concentrations and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate status were determined. Mean daily vitamin B-6 and protein intakes of the subjects were 1.48 mg and 78.3 g, respectively. The ranges for plasma B-6 vitamer and 4-PA concentrations for these subjects which had seemingly adequate vitamin B-6 status were as follows: (nmol/L) PLP, 40.9-122.2; PNP, 0-16.1; PMP, 0-8.1; PL, 0- 15.0; PN, 0-21.9; PM, 0-17.8; and 4-PA, 0-55.7. PLP was the predominant plasma B-6 vitamer as well as being the only vitamer found in plasma of all subjects. Urinary 4-PA concentrations of the girls ranged from 0.11-2.50 pmol/mmol creatinine. The B-6 vitamer values of these white adolescent girls should be of use in the establishment of normal ranges for vitamin B-6 status parameters. HPLC methodologies seem to be advantageous for the rapid and accurate assessment of vitamin B-6 status."],"dc:description.degree":["Ph. D."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/10919/53934"],"dc:language.iso":["en_US"],"dc:publisher":["Virginia Polytechnic Institute and State University"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Separation and quantitation of the seven forms of vitamin B-6 in plasma and 4-pyridoxic acid in urine of adolescent girls by reverse phase high performance liquid chromatography"],"dc:type":["Dissertation"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Human Nutrition and Foods"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Ph. D."],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:26Z"}