Changes in respiration rates and biomass attributes of epilithon due to extended exposure to zinc

The purpose of this research was to determine the influence of extended dosing of zinc on the carbon cycling and biomass characteristics of freshwater epilithon. Experiments were conducted in artificial streams continuously dosed with 0.00, 0.05, or 1.00 mg Zn liter⁻¹ for 20 to 30 days during summer and fall, 1984 and 1985. Repeated measurement of epilithon structure and function included estimates of ¹⁴C-glucose respiration, ¹⁴C-glutamate respiration, O₂ and CO₂ flux rates, ash-free dry weight (AFDW), protein, carbohydrate, and algal pigment concentrations, and total and zinc-tolerant colony forming units. An increase in epilithic glucose respiration per unit biomass consistently occurred 5 to 10 days after dosing with 1.0 mg Zn liter⁻¹ was started. At the same time significantly lower epilithon biomass occurred in the high dosed streams relative to controls in 3 out of 4 studies. Although algal pigment concentrations were lowest in the high dose streams at the midpoint of the studies, the chlorophyll a-to-pheophytin a ratio remained high, indicating that the minimal algal population was not senescing in situ. After 30 days, the epilithon dosed with 1.0 mg Zn liter⁻¹ had higher AFDW, protein, and carbohydrate concentrations than the other treatments. By 20 days, the high zinc treatment showed evidence of more total and zinc-tolerant colony forming units and lower rates of O₂ and CO₂ flux than epilithon from control streams. The high rates of glucose respiration were characteristic of epilithic communities stressed by 1.0 mg Zn liter⁻¹, and this response was not apparently due to in situ senescence of zinc-sensitive cells; the results suggested that epilithic biomass was washed out of the systems, not being degraded in situ. The development of unique epilithon communities that are acclimated to prolonged zinc exposure is evident in the eventual recolonization of the artificial surfaces, glucose respiration rates that are comparable to controls, and presence of zinc-tolerant heterotrophs.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Microbiology

Department dc:contributor.department

Microbiology

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1986

Author dc:creator

• Colwell, Frederic S.

Chairs dc:contributor.committeechair

• Horner, S.G.
• Cherry, D.S.

Committee members dc:contributor.committeemember

• Benoit, Robert E.
• Krieg, Noel R.
• Webster, Jackson R.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/10919/53618

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/53618