{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/49673"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/49673","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Characterization of Value Added Proteins and Lipids form Microalgae","abstract":"Microalgae have been so far identified as the major producers of organic matter through their photosynthetic activities. In the present work, Nannochloris sp. and Amphora sp., two marine microalgae, have been investigated for proteins and lipids production. Protein fraction was quantified using Bicinchoninic acid (BCA) assay. Protein content in Nannochloris sp. was 16.69 ±4.07 % of dry mass and in Amphora sp. it was 39.89 ±2.09 % of dry mass. Enzyme assays were conducted spectrophotometrically. Nannochloris sp. had malate dehydrogenase, peroxidase and catalase activities. Amphora sp. exhibited malate dehydrogenase, catalase and cytochrome C oxidase activities. These enzymes have several valuable applications in some metabolic pathways and as antioxidant nutrition additives. Besides, lipid extraction was conducted using methanol/ chloroform solvent extraction. Crude lipid extract was analyzed using gas chromatography-mass spectrometry. Lipid contents were 8.14 ±3.67 % in Nannochloris sp. and 10.48 ±1.26% on dry basis in Amphora sp., respectively. Nannochloris sp. fatty acids were composed of C16:0 and C18:0 that are valuable for biodiesel production, and É-3 C18:3, É-6 C18:2, É-6 C16:2 having great nutritional values. In Amphora sp., the fatty acids consisted of C14:0, C16:0 and C16:1 shown to be valuable for biodiesel production and É-3 C22:6 having high nutritional values. Furthermore, a single step conversion of microalgal oil to fatty acid methyl esters was carried out starting directly from lyophilized microalgae. This promising process, in situ transesterification, led to better yields of methyl esters as compared to conventional lipid extraction followed by separate transesterification.","abstract_html":"Microalgae have been so far identified as the major producers of organic matter through their photosynthetic activities. In the present work, Nannochloris sp. and Amphora sp., two marine microalgae, have been investigated for proteins and lipids production. Protein fraction was quantified using Bicinchoninic acid (BCA) assay. Protein content in Nannochloris sp. was 16.69 ±4.07 % of dry mass and in Amphora sp. it was 39.89 ±2.09 % of dry mass. Enzyme assays were conducted spectrophotometrically. Nannochloris sp. had malate dehydrogenase, peroxidase and catalase activities. Amphora sp. exhibited malate dehydrogenase, catalase and cytochrome C oxidase activities. These enzymes have several valuable applications in some metabolic pathways and as antioxidant nutrition additives. Besides, lipid extraction was conducted using methanol/ chloroform solvent extraction. Crude lipid extract was analyzed using gas chromatography-mass spectrometry. Lipid contents were 8.14 ±3.67 % in Nannochloris sp. and 10.48 ±1.26% on dry basis in Amphora sp., respectively. Nannochloris sp. fatty acids were composed of C16:0 and C18:0 that are valuable for biodiesel production, and É-3 C18:3, É-6 C18:2, É-6 C16:2 having great nutritional values. In Amphora sp., the fatty acids consisted of C14:0, C16:0 and C16:1 shown to be valuable for biodiesel production and É-3 C22:6 having high nutritional values. Furthermore, a single step conversion of microalgal oil to fatty acid methyl esters was carried out starting directly from lyophilized microalgae. This promising process, in situ transesterification, led to better yields of methyl esters as compared to conventional lipid extraction followed by separate transesterification.","abstract_has_math":false,"creators":["Khili, Mouna"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Biological Systems Engineering","degree_department":"Biological Systems Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Zhang, Chenming Mike"],"committee_members":["Agblevor, Foster A.","Sayadi, Sami","Senger, Ryan S."],"year":2013,"date_issued":"2013-01-30","date_published":"2013-01-30","updated_at":"2026-07-22T22:18:52Z","subjects":["microalgae","enzyme assays","lipids analysis","transesterification"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:322"],"render_values":[{"text":"vt_gsexam:322","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/49673","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Zhang, Chenming Mike"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Agblevor, Foster A.","Sayadi, Sami","Senger, Ryan S."]},{"key":"dc:contributor.department","label":"Department","values":["Biological Systems Engineering"]},{"key":"dc:creator","label":"Author","values":["Khili, Mouna"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-07-25T06:00:09Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-07-25T06:00:09Z"]},{"key":"dc:date.issued","label":"Date","values":["2013-01-30"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Systems Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["microalgae","enzyme assays","lipids analysis","transesterification"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["vt_gsexam:322"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/49673"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Microalgae have been so far identified as the major producers of organic matter through their photosynthetic activities. In the present work, Nannochloris sp. and Amphora sp., two marine microalgae, have been investigated for proteins and lipids production. Protein fraction was quantified using Bicinchoninic acid (BCA) assay. Protein content in Nannochloris sp. was 16.69 ±4.07 % of dry mass and in Amphora sp. it was 39.89 ±2.09 % of dry mass. Enzyme assays were conducted spectrophotometrically. Nannochloris sp. had malate dehydrogenase, peroxidase and catalase activities. Amphora sp. exhibited malate dehydrogenase, catalase and cytochrome C oxidase activities. These enzymes have several valuable applications in some metabolic pathways and as antioxidant nutrition additives. Besides, lipid extraction was conducted using methanol/ chloroform solvent extraction. Crude lipid extract was analyzed using gas chromatography-mass spectrometry. Lipid contents were 8.14 ±3.67 % in Nannochloris sp. and 10.48 ±1.26% on dry basis in Amphora sp., respectively. Nannochloris sp. fatty acids were composed of C16:0 and C18:0 that are valuable for biodiesel production, and É-3 C18:3, É-6 C18:2, É-6 C16:2 having great nutritional values. In Amphora sp., the fatty acids consisted of C14:0, C16:0 and C16:1 shown to be valuable for biodiesel production and É-3 C22:6 having high nutritional values. Furthermore, a single step conversion of microalgal oil to fatty acid methyl esters was carried out starting directly from lyophilized microalgae. This promising process, in situ transesterification, led to better yields of methyl esters as compared to conventional lipid extraction followed by separate transesterification."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["ETD"]},{"key":"dc:title","label":"Title","values":["Characterization of Value Added Proteins and Lipids form Microalgae"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Zhang, Chenming Mike"],"dc:contributor.committeemember":["Agblevor, Foster A.","Sayadi, Sami","Senger, Ryan S."],"dc:contributor.department":["Biological Systems Engineering"],"dc:creator":["Khili, Mouna"],"dc:date.accessioned":["2014-07-25T06:00:09Z"],"dc:date.available":["2014-07-25T06:00:09Z"],"dc:date.issued":["2013-01-30"],"dc:description.abstract":["Microalgae have been so far identified as the major producers of organic matter through their photosynthetic activities. In the present work, Nannochloris sp. and Amphora sp., two marine microalgae, have been investigated for proteins and lipids production. Protein fraction was quantified using Bicinchoninic acid (BCA) assay. Protein content in Nannochloris sp. was 16.69 ±4.07 % of dry mass and in Amphora sp. it was 39.89 ±2.09 % of dry mass. Enzyme assays were conducted spectrophotometrically. Nannochloris sp. had malate dehydrogenase, peroxidase and catalase activities. Amphora sp. exhibited malate dehydrogenase, catalase and cytochrome C oxidase activities. These enzymes have several valuable applications in some metabolic pathways and as antioxidant nutrition additives. Besides, lipid extraction was conducted using methanol/ chloroform solvent extraction. Crude lipid extract was analyzed using gas chromatography-mass spectrometry. Lipid contents were 8.14 ±3.67 % in Nannochloris sp. and 10.48 ±1.26% on dry basis in Amphora sp., respectively. Nannochloris sp. fatty acids were composed of C16:0 and C18:0 that are valuable for biodiesel production, and É-3 C18:3, É-6 C18:2, É-6 C16:2 having great nutritional values. In Amphora sp., the fatty acids consisted of C14:0, C16:0 and C16:1 shown to be valuable for biodiesel production and É-3 C22:6 having high nutritional values. Furthermore, a single step conversion of microalgal oil to fatty acid methyl esters was carried out starting directly from lyophilized microalgae. This promising process, in situ transesterification, led to better yields of methyl esters as compared to conventional lipid extraction followed by separate transesterification."],"dc:description.degree":["Master of Science"],"dc:format.medium":["ETD"],"dc:identifier.other":["vt_gsexam:322"],"dc:identifier.uri":["http://hdl.handle.net/10919/49673"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["microalgae","enzyme assays","lipids analysis","transesterification"],"dc:title":["Characterization of Value Added Proteins and Lipids form Microalgae"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biological Systems Engineering"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:18:52Z"}