{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/46484"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/46484","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Effects of Acute Nutritional Deprivation on Lymphocyte Subsets and Membrane Function in Cats","abstract":"Identification of patients with suboptimal nutritional status allows for early treatment intervention. Currently, no definitive test of nutritional status exists. Therefore, this study was conducted to identify possible functional indicators of acute nutritional deprivation. The effects of total nutritional deprivation and subsequent refeeding on lymphocyte functions and subpopulations were examined in 23 healthy cats. Peripheral blood samples were analyzed at various times during fasting and refeeding periods. During the fasting period, decreases were observed in leukocyte number (day 4; p < 0.04), lymphocyte number (p < 0.02), CD4+ cells (day 4; p < 0.06), CD4:CD8 ratio (0 hours; p < 0.004), and mitogen stimulated CD4:CD8 ratio (72 hours; p < 0.15) during the fasting period as compared to baseline. Increases were seen in CD4+ cells (day 7; p < 0.09), CD8+ cells (day 7; p < 0.04) and intracellular calcium (day 4; p < 0.02) as compared to baseline. During the refeeding period increases (p < 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p < 0.07) and lymphocyte number (p < 0.004) as compared to day 7. These findings suggest that 7 days starvation had immunosuppressive effects on cats which were alleviated during 7 days refeeding. The use of CD4:CD8 ratio in conjunction with intracellular calcium flux may be useful as indices of nutritional status.","abstract_html":"Identification of patients with suboptimal nutritional status allows for early treatment intervention. Currently, no definitive test of nutritional status exists. Therefore, this study was conducted to identify possible functional indicators of acute nutritional deprivation. The effects of total nutritional deprivation and subsequent refeeding on lymphocyte functions and subpopulations were examined in 23 healthy cats. Peripheral blood samples were analyzed at various times during fasting and refeeding periods. During the fasting period, decreases were observed in leukocyte number (day 4; p &lt; 0.04), lymphocyte number (p &lt; 0.02), CD4+ cells (day 4; p &lt; 0.06), CD4:CD8 ratio (0 hours; p &lt; 0.004), and mitogen stimulated CD4:CD8 ratio (72 hours; p &lt; 0.15) during the fasting period as compared to baseline. Increases were seen in CD4+ cells (day 7; p &lt; 0.09), CD8+ cells (day 7; p &lt; 0.04) and intracellular calcium (day 4; p &lt; 0.02) as compared to baseline. During the refeeding period increases (p &lt; 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p &lt; 0.07) and lymphocyte number (p &lt; 0.004) as compared to day 7. These findings suggest that 7 days starvation had immunosuppressive effects on cats which were alleviated during 7 days refeeding. The use of CD4:CD8 ratio in conjunction with intracellular calcium flux may be useful as indices of nutritional status.","abstract_has_math":false,"creators":["Freitag, Kimberly A."],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Human Nutrition, Foods, and Exercise","degree_department":"Human Nutrition, Foods, and Exercise","school":null,"contributors":[],"advisors":[],"committee_chairs":["Thomas, Elizabeth A."],"committee_members":["Barbeau, William E.","Saker, Korinn E."],"year":1998,"date_issued":"1998-04-10","date_published":"1998-04-10","updated_at":"2026-07-22T22:19:18Z","subjects":["CD4","CD8","starvation","calcium"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-32998-105852"],"render_values":[{"text":"etd-32998-105852","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/46484","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Thomas, Elizabeth A."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Barbeau, William E.","Saker, Korinn E."]},{"key":"dc:contributor.department","label":"Department","values":["Human Nutrition, Foods, and Exercise"]},{"key":"dc:creator","label":"Author","values":["Freitag, Kimberly A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:52:54Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:52:54Z","1999-04-29"]},{"key":"dc:date.issued","label":"Date","values":["1998-04-10"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Human Nutrition, Foods, and Exercise"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["CD4","CD8","starvation","calcium"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-32998-105852"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/46484"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Identification of patients with suboptimal nutritional status allows for early treatment intervention. Currently, no definitive test of nutritional status exists. Therefore, this study was conducted to identify possible functional indicators of acute nutritional deprivation. The effects of total nutritional deprivation and subsequent refeeding on lymphocyte functions and subpopulations were examined in 23 healthy cats. Peripheral blood samples were analyzed at various times during fasting and refeeding periods. During the fasting period, decreases were observed in leukocyte number (day 4; p < 0.04), lymphocyte number (p < 0.02), CD4+ cells (day 4; p < 0.06), CD4:CD8 ratio (0 hours; p < 0.004), and mitogen stimulated CD4:CD8 ratio (72 hours; p < 0.15) during the fasting period as compared to baseline. Increases were seen in CD4+ cells (day 7; p < 0.09), CD8+ cells (day 7; p < 0.04) and intracellular calcium (day 4; p < 0.02) as compared to baseline. During the refeeding period increases (p < 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p < 0.07) and lymphocyte number (p < 0.004) as compared to day 7. These findings suggest that 7 days starvation had immunosuppressive effects on cats which were alleviated during 7 days refeeding. The use of CD4:CD8 ratio in conjunction with intracellular calcium flux may be useful as indices of nutritional status."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Effects of Acute Nutritional Deprivation on Lymphocyte Subsets and Membrane Function in Cats"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Thomas, Elizabeth A."],"dc:contributor.committeemember":["Barbeau, William E.","Saker, Korinn E."],"dc:contributor.department":["Human Nutrition, Foods, and Exercise"],"dc:creator":["Freitag, Kimberly A."],"dc:date.accessioned":["2014-03-14T21:52:54Z"],"dc:date.available":["2014-03-14T21:52:54Z","1999-04-29"],"dc:date.issued":["1998-04-10"],"dc:description.abstract":["Identification of patients with suboptimal nutritional status allows for early treatment intervention. Currently, no definitive test of nutritional status exists. Therefore, this study was conducted to identify possible functional indicators of acute nutritional deprivation. The effects of total nutritional deprivation and subsequent refeeding on lymphocyte functions and subpopulations were examined in 23 healthy cats. Peripheral blood samples were analyzed at various times during fasting and refeeding periods. During the fasting period, decreases were observed in leukocyte number (day 4; p < 0.04), lymphocyte number (p < 0.02), CD4+ cells (day 4; p < 0.06), CD4:CD8 ratio (0 hours; p < 0.004), and mitogen stimulated CD4:CD8 ratio (72 hours; p < 0.15) during the fasting period as compared to baseline. Increases were seen in CD4+ cells (day 7; p < 0.09), CD8+ cells (day 7; p < 0.04) and intracellular calcium (day 4; p < 0.02) as compared to baseline. During the refeeding period increases (p < 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p < 0.07) and lymphocyte number (p < 0.004) as compared to day 7. These findings suggest that 7 days starvation had immunosuppressive effects on cats which were alleviated during 7 days refeeding. The use of CD4:CD8 ratio in conjunction with intracellular calcium flux may be useful as indices of nutritional status."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-32998-105852"],"dc:identifier.uri":["http://hdl.handle.net/10919/46484"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["CD4","CD8","starvation","calcium"],"dc:title":["Effects of Acute Nutritional Deprivation on Lymphocyte Subsets and Membrane Function in Cats"],"dc:type":["Thesis"],"thesis:degree_discipline":["Human Nutrition, Foods, and Exercise"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:18Z"}