{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/46408"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/46408","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Tracking soybean mosaic virus movement in soybean by leaf imprint immunoassay","abstract":"The responses of soybean (Glycine max (L.) Merr.) to soybean mosaic virus (SMV) include mosaic, necrosis and no symptoms, and vary with virus strain and allele of the gene for resistance (Rsv) carried by the host. Genetic studies have shown that plants giving mosaic are susceptible, and that plants giving either the necrotic or symptomless response are resistant. The objective of this study was to examine the mechanism of resistance by tracking SMV replication and movement in susceptible responses, and its restriction in necrotic and symptomless responses. Two SMV strains were inoculated at a single spot to Rsv-containing genotypes, selected to give each response. The leaf imprint immunoassay was developed and used to track the rate and extent of invasion by SMV from the tip of a primary, unifoliolate leaf to regions within that leaf and to the stem and younger trifoliolate leaves. In susceptible responses, SMV was detected at the site of inoculation in 6-7 days, and throughout the mid-rib and in the first trifoliate leaf in 8-9 days. In necrotic resistance responses, SMV was detected at and around the inoculation site in 8-9 days and in leaf veins, midrib and the first trifoliolate in 15-17 days, but was restricted to necrotic areas. In symptomless resistance responses, no virus was detected at any time. Greater virus replication and movement was found in unifoliolate and trifoliolate leaves of younger (15 days-old) than of older (18-days-old) plants. It was concluded that: (i) necrotic ally-responding cultivars manifest resistance by reduction and delay in replication, and by restriction of virus movement; (ii) virus replication is restricted in resistant cultivars; and (iii) rate and extent virus replication and movement is affected by stage of plant development.","abstract_html":"The responses of soybean (Glycine max (L.) Merr.) to soybean mosaic virus (SMV) include mosaic, necrosis and no symptoms, and vary with virus strain and allele of the gene for resistance (Rsv) carried by the host. Genetic studies have shown that plants giving mosaic are susceptible, and that plants giving either the necrotic or symptomless response are resistant. The objective of this study was to examine the mechanism of resistance by tracking SMV replication and movement in susceptible responses, and its restriction in necrotic and symptomless responses. Two SMV strains were inoculated at a single spot to Rsv-containing genotypes, selected to give each response. The leaf imprint immunoassay was developed and used to track the rate and extent of invasion by SMV from the tip of a primary, unifoliolate leaf to regions within that leaf and to the stem and younger trifoliolate leaves. In susceptible responses, SMV was detected at the site of inoculation in 6-7 days, and throughout the mid-rib and in the first trifoliate leaf in 8-9 days. In necrotic resistance responses, SMV was detected at and around the inoculation site in 8-9 days and in leaf veins, midrib and the first trifoliolate in 15-17 days, but was restricted to necrotic areas. In symptomless resistance responses, no virus was detected at any time. Greater virus replication and movement was found in unifoliolate and trifoliolate leaves of younger (15 days-old) than of older (18-days-old) plants. It was concluded that: (i) necrotic ally-responding cultivars manifest resistance by reduction and delay in replication, and by restriction of virus movement; (ii) virus replication is restricted in resistant cultivars; and (iii) rate and extent virus replication and movement is affected by stage of plant development.","abstract_has_math":false,"creators":["Gera, Tarun"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Plant Pathology, Physiology, and Weed Science","degree_department":"Plant Pathology, Physiology, and Weed Science","school":null,"contributors":[],"advisors":[],"committee_chairs":["Tolin, Sue A."],"committee_members":["Warren, Herman L.","Buss, Glenn R."],"year":1994,"date_issued":"1994-02-15","date_published":"1994-02-15","updated_at":"2026-07-22T22:19:32Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12232009-020606"],"render_values":[{"text":"etd-12232009-020606","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/46408","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Tolin, Sue A."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Warren, Herman L.","Buss, Glenn R."]},{"key":"dc:contributor.department","label":"Department","values":["Plant Pathology, Physiology, and Weed Science"]},{"key":"dc:creator","label":"Author","values":["Gera, Tarun"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:52:28Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:52:28Z","2009-12-23"]},{"key":"dc:date.issued","label":"Date","values":["1994-02-15"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Pathology, Physiology, and Weed Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12232009-020606"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/46408"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The responses of soybean (Glycine max (L.) Merr.) to soybean mosaic virus (SMV) include mosaic, necrosis and no symptoms, and vary with virus strain and allele of the gene for resistance (Rsv) carried by the host. Genetic studies have shown that plants giving mosaic are susceptible, and that plants giving either the necrotic or symptomless response are resistant. The objective of this study was to examine the mechanism of resistance by tracking SMV replication and movement in susceptible responses, and its restriction in necrotic and symptomless responses. Two SMV strains were inoculated at a single spot to Rsv-containing genotypes, selected to give each response. The leaf imprint immunoassay was developed and used to track the rate and extent of invasion by SMV from the tip of a primary, unifoliolate leaf to regions within that leaf and to the stem and younger trifoliolate leaves. In susceptible responses, SMV was detected at the site of inoculation in 6-7 days, and throughout the mid-rib and in the first trifoliate leaf in 8-9 days. In necrotic resistance responses, SMV was detected at and around the inoculation site in 8-9 days and in leaf veins, midrib and the first trifoliolate in 15-17 days, but was restricted to necrotic areas. In symptomless resistance responses, no virus was detected at any time. Greater virus replication and movement was found in unifoliolate and trifoliolate leaves of younger (15 days-old) than of older (18-days-old) plants. It was concluded that: (i) necrotic ally-responding cultivars manifest resistance by reduction and delay in replication, and by restriction of virus movement; (ii) virus replication is restricted in resistant cultivars; and (iii) rate and extent virus replication and movement is affected by stage of plant development."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Tracking soybean mosaic virus movement in soybean by leaf imprint immunoassay"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Tolin, Sue A."],"dc:contributor.committeemember":["Warren, Herman L.","Buss, Glenn R."],"dc:contributor.department":["Plant Pathology, Physiology, and Weed Science"],"dc:creator":["Gera, Tarun"],"dc:date.accessioned":["2014-03-14T21:52:28Z"],"dc:date.available":["2014-03-14T21:52:28Z","2009-12-23"],"dc:date.issued":["1994-02-15"],"dc:description.abstract":["The responses of soybean (Glycine max (L.) Merr.) to soybean mosaic virus (SMV) include mosaic, necrosis and no symptoms, and vary with virus strain and allele of the gene for resistance (Rsv) carried by the host. Genetic studies have shown that plants giving mosaic are susceptible, and that plants giving either the necrotic or symptomless response are resistant. The objective of this study was to examine the mechanism of resistance by tracking SMV replication and movement in susceptible responses, and its restriction in necrotic and symptomless responses. Two SMV strains were inoculated at a single spot to Rsv-containing genotypes, selected to give each response. The leaf imprint immunoassay was developed and used to track the rate and extent of invasion by SMV from the tip of a primary, unifoliolate leaf to regions within that leaf and to the stem and younger trifoliolate leaves. In susceptible responses, SMV was detected at the site of inoculation in 6-7 days, and throughout the mid-rib and in the first trifoliate leaf in 8-9 days. In necrotic resistance responses, SMV was detected at and around the inoculation site in 8-9 days and in leaf veins, midrib and the first trifoliolate in 15-17 days, but was restricted to necrotic areas. In symptomless resistance responses, no virus was detected at any time. Greater virus replication and movement was found in unifoliolate and trifoliolate leaves of younger (15 days-old) than of older (18-days-old) plants. It was concluded that: (i) necrotic ally-responding cultivars manifest resistance by reduction and delay in replication, and by restriction of virus movement; (ii) virus replication is restricted in resistant cultivars; and (iii) rate and extent virus replication and movement is affected by stage of plant development."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-12232009-020606"],"dc:identifier.uri":["http://hdl.handle.net/10919/46408"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Tracking soybean mosaic virus movement in soybean by leaf imprint immunoassay"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Plant Pathology, Physiology, and Weed Science"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:32Z"}