{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/46232"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/46232","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Enhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy products","abstract":"<u>Bifidobacterium</u> spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, <u>Bifidobacterium</u> spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI's anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods. <u>Bifidobacterium adolescentis</u> cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction.","abstract_html":"&lt;u&gt;Bifidobacterium&lt;/u&gt; spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, &lt;u&gt;Bifidobacterium&lt;/u&gt; spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI&#x27;s anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods. &lt;u&gt;Bifidobacterium adolescentis&lt;/u&gt; cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction.","abstract_has_math":false,"creators":["Arany, Catherine Beatrice"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Food Science and Technology","degree_department":"Food Science and Technology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Hackney, Cameron Raj"],"committee_members":["Pierson, Merle D.","Eigel, William N. III"],"year":1992,"date_issued":"1992-09-03","date_published":"1992-09-03","updated_at":"2026-07-22T22:20:29Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12162009-020131"],"render_values":[{"text":"etd-12162009-020131","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/46232","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Hackney, Cameron Raj"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Pierson, Merle D.","Eigel, William N. III"]},{"key":"dc:contributor.department","label":"Department","values":["Food Science and Technology"]},{"key":"dc:creator","label":"Author","values":["Arany, Catherine Beatrice"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:51:29Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:51:29Z","2009-12-16"]},{"key":"dc:date.issued","label":"Date","values":["1992-09-03"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science and Technology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12162009-020131"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/46232"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["<u>Bifidobacterium</u> spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, <u>Bifidobacterium</u> spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI's anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods. <u>Bifidobacterium adolescentis</u> cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Enhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy products"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Hackney, Cameron Raj"],"dc:contributor.committeemember":["Pierson, Merle D.","Eigel, William N. III"],"dc:contributor.department":["Food Science and Technology"],"dc:creator":["Arany, Catherine Beatrice"],"dc:date.accessioned":["2014-03-14T21:51:29Z"],"dc:date.available":["2014-03-14T21:51:29Z","2009-12-16"],"dc:date.issued":["1992-09-03"],"dc:description.abstract":["<u>Bifidobacterium</u> spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, <u>Bifidobacterium</u> spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI's anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods. <u>Bifidobacterium adolescentis</u> cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-12162009-020131"],"dc:identifier.uri":["http://hdl.handle.net/10919/46232"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Enhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy products"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Food Science and Technology"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:29Z"}