{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/46122"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/46122","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The urinary excretion of mercapturic acids in free-living adult males","abstract":"In order to establish a profile of detoxification via glutathione conjugation, the level of urinary mercapturic acid excreted by a free-living male population and the effect of external environmental and genetic factors, such as consumption of vegetables, fruits, and meat, charbroiled food intake, tobacco, alcohol, caffeinated coffee, and marijuana use, exposure to chemicals and familial cancer incidence, were investigated. A subgroup of 30 subjects was randomly selected from 117 subjects who complied with the collection protocol. Three consecutive 24 hr urine samples of this subgroup were analyzed. The modified method of Seutter-Berlage et al (Chemical Porphyria in Man. Elsevier/North-Holland Biomedical Press, N.Y. 1979:233-236) was used for the quantitation of urinary mercapturic acid. The mean excretion of mercapturic acid was 0.27 mmole mercapturate (-SH)/24 hr and 18.1 umole -SH/mmole creatinine. An analysis of variance showed a large degree of inter- and intraindividual variability. The interindividual coefficients of variation in mmole -SH/24 hr and umole -SH/mmole creatinine were 37.7% and 31.2%, respectively. The intraindividual coefficients of variation in mmole -SH/24 hr and umole - SH/mmole creatinine were 32.4% and 30.1%, respectively. A higher (p≤0.05) excretion of mercapturic acid was observed among subjects with a high frequency of exposure to chemicals. The lack of significance of the other dietary, non-dietary, and genetic factors on the observed mercapturic acid excretion may be due to the large inter- and intravariability, the use of food consumption frequency in food intake analysis, and unequal sample sizes of subgroups.","abstract_html":"In order to establish a profile of detoxification via glutathione conjugation, the level of urinary mercapturic acid excreted by a free-living male population and the effect of external environmental and genetic factors, such as consumption of vegetables, fruits, and meat, charbroiled food intake, tobacco, alcohol, caffeinated coffee, and marijuana use, exposure to chemicals and familial cancer incidence, were investigated. A subgroup of 30 subjects was randomly selected from 117 subjects who complied with the collection protocol. Three consecutive 24 hr urine samples of this subgroup were analyzed. The modified method of Seutter-Berlage et al (Chemical Porphyria in Man. Elsevier/North-Holland Biomedical Press, N.Y. 1979:233-236) was used for the quantitation of urinary mercapturic acid. The mean excretion of mercapturic acid was 0.27 mmole mercapturate (-SH)/24 hr and 18.1 umole -SH/mmole creatinine. An analysis of variance showed a large degree of inter- and intraindividual variability. The interindividual coefficients of variation in mmole -SH/24 hr and umole -SH/mmole creatinine were 37.7% and 31.2%, respectively. The intraindividual coefficients of variation in mmole -SH/24 hr and umole - SH/mmole creatinine were 32.4% and 30.1%, respectively. A higher (p≤0.05) excretion of mercapturic acid was observed among subjects with a high frequency of exposure to chemicals. The lack of significance of the other dietary, non-dietary, and genetic factors on the observed mercapturic acid excretion may be due to the large inter- and intravariability, the use of food consumption frequency in food intake analysis, and unequal sample sizes of subgroups.","abstract_has_math":false,"creators":["Chen, Hui-Chuen"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Human Nutrition and Foods","degree_department":"Human Nutrition and Foods","school":null,"contributors":[],"advisors":[],"committee_chairs":["Webb, Ryland E."],"committee_members":["Thye, Forrest W.","Bunce, George Edwin"],"year":1991,"date_issued":"1991-12-17","date_published":"1991-12-17","updated_at":"2026-07-22T22:20:38Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12052009-020010"],"render_values":[{"text":"etd-12052009-020010","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/46122","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Webb, Ryland E."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Thye, Forrest W.","Bunce, George Edwin"]},{"key":"dc:contributor.department","label":"Department","values":["Human Nutrition and Foods"]},{"key":"dc:creator","label":"Author","values":["Chen, Hui-Chuen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:51:01Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:51:01Z","2009-12-05"]},{"key":"dc:date.issued","label":"Date","values":["1991-12-17"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Human Nutrition and Foods"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-12052009-020010"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/46122"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In order to establish a profile of detoxification via glutathione conjugation, the level of urinary mercapturic acid excreted by a free-living male population and the effect of external environmental and genetic factors, such as consumption of vegetables, fruits, and meat, charbroiled food intake, tobacco, alcohol, caffeinated coffee, and marijuana use, exposure to chemicals and familial cancer incidence, were investigated. A subgroup of 30 subjects was randomly selected from 117 subjects who complied with the collection protocol. Three consecutive 24 hr urine samples of this subgroup were analyzed. The modified method of Seutter-Berlage et al (Chemical Porphyria in Man. Elsevier/North-Holland Biomedical Press, N.Y. 1979:233-236) was used for the quantitation of urinary mercapturic acid. The mean excretion of mercapturic acid was 0.27 mmole mercapturate (-SH)/24 hr and 18.1 umole -SH/mmole creatinine. An analysis of variance showed a large degree of inter- and intraindividual variability. The interindividual coefficients of variation in mmole -SH/24 hr and umole -SH/mmole creatinine were 37.7% and 31.2%, respectively. The intraindividual coefficients of variation in mmole -SH/24 hr and umole - SH/mmole creatinine were 32.4% and 30.1%, respectively. A higher (p≤0.05) excretion of mercapturic acid was observed among subjects with a high frequency of exposure to chemicals. The lack of significance of the other dietary, non-dietary, and genetic factors on the observed mercapturic acid excretion may be due to the large inter- and intravariability, the use of food consumption frequency in food intake analysis, and unequal sample sizes of subgroups."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The urinary excretion of mercapturic acids in free-living adult males"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Webb, Ryland E."],"dc:contributor.committeemember":["Thye, Forrest W.","Bunce, George Edwin"],"dc:contributor.department":["Human Nutrition and Foods"],"dc:creator":["Chen, Hui-Chuen"],"dc:date.accessioned":["2014-03-14T21:51:01Z"],"dc:date.available":["2014-03-14T21:51:01Z","2009-12-05"],"dc:date.issued":["1991-12-17"],"dc:description.abstract":["In order to establish a profile of detoxification via glutathione conjugation, the level of urinary mercapturic acid excreted by a free-living male population and the effect of external environmental and genetic factors, such as consumption of vegetables, fruits, and meat, charbroiled food intake, tobacco, alcohol, caffeinated coffee, and marijuana use, exposure to chemicals and familial cancer incidence, were investigated. A subgroup of 30 subjects was randomly selected from 117 subjects who complied with the collection protocol. Three consecutive 24 hr urine samples of this subgroup were analyzed. The modified method of Seutter-Berlage et al (Chemical Porphyria in Man. Elsevier/North-Holland Biomedical Press, N.Y. 1979:233-236) was used for the quantitation of urinary mercapturic acid. The mean excretion of mercapturic acid was 0.27 mmole mercapturate (-SH)/24 hr and 18.1 umole -SH/mmole creatinine. An analysis of variance showed a large degree of inter- and intraindividual variability. The interindividual coefficients of variation in mmole -SH/24 hr and umole -SH/mmole creatinine were 37.7% and 31.2%, respectively. The intraindividual coefficients of variation in mmole -SH/24 hr and umole - SH/mmole creatinine were 32.4% and 30.1%, respectively. A higher (p≤0.05) excretion of mercapturic acid was observed among subjects with a high frequency of exposure to chemicals. The lack of significance of the other dietary, non-dietary, and genetic factors on the observed mercapturic acid excretion may be due to the large inter- and intravariability, the use of food consumption frequency in food intake analysis, and unequal sample sizes of subgroups."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-12052009-020010"],"dc:identifier.uri":["http://hdl.handle.net/10919/46122"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["The urinary excretion of mercapturic acids in free-living adult males"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Human Nutrition and Foods"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:38Z"}