{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/45965"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/45965","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Oxygen toxicity in Campylobacter jejuni: physiological comparison of a microaerophilic wild-type strain with an aerotolerant mutant","abstract":"A comparative study of the microaerophilic <u>Campylobacter jejuni</u> strain H840 with an aerotolerant mutant, MC711-01, revealed that catalase and, to a lesser extent, SOD activity is correlated with the aerotolerance and enhanced resistance of MC7ll-0l to H₂O₂. When cells were cultured under 6% oxygen, the specific activity of catalase was significantly higher in crude extracts of MC711-01 than of H840. The catalase activity of MC711-01 more than doubled when cells were cultured under 21% oxygen, and this activity was 2.6 times greater than that of H84O; no corresponding increase was observed in strain H840. There was no significant difference in the mean SOD activity of the two strains when cultured under 6% oxygen or in H840 cells cultured under either 6% or 21% oxygen; however, the SOD activity of MC711-01 increased 1.5 times when cells were cultured under 21% oxygen. Survival studies revealed that MC711-01 was significantly more E resistant to H₂O₂ when cultured under either 6% or 21% oxygen. However, both MC711-01 and H84O were more susceptible to H₂O₂ when grown under 21% oxygen, indicating that both strains might be more highly stressed when the cells are grown at this oxygen tension. The present study suggests that in <u>C. jejuni</u>, the level of catalase activity may influence the degree of susceptibility to H₂O₂ and consequently, the degree of aerotolerance.","abstract_html":"A comparative study of the microaerophilic &lt;u&gt;Campylobacter jejuni&lt;/u&gt; strain H840 with an aerotolerant mutant, MC711-01, revealed that catalase and, to a lesser extent, SOD activity is correlated with the aerotolerance and enhanced resistance of MC7ll-0l to H₂O₂. When cells were cultured under 6% oxygen, the specific activity of catalase was significantly higher in crude extracts of MC711-01 than of H840. The catalase activity of MC711-01 more than doubled when cells were cultured under 21% oxygen, and this activity was 2.6 times greater than that of H84O; no corresponding increase was observed in strain H840. There was no significant difference in the mean SOD activity of the two strains when cultured under 6% oxygen or in H840 cells cultured under either 6% or 21% oxygen; however, the SOD activity of MC711-01 increased 1.5 times when cells were cultured under 21% oxygen. Survival studies revealed that MC711-01 was significantly more E resistant to H₂O₂ when cultured under either 6% or 21% oxygen. However, both MC711-01 and H84O were more susceptible to H₂O₂ when grown under 21% oxygen, indicating that both strains might be more highly stressed when the cells are grown at this oxygen tension. The present study suggests that in &lt;u&gt;C. jejuni&lt;/u&gt;, the level of catalase activity may influence the degree of susceptibility to H₂O₂ and consequently, the degree of aerotolerance.","abstract_has_math":false,"creators":["Vercellone, Pamela Ann"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Microbiology","degree_department":"Microbiology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Krieg, Noel R."],"committee_members":["Smibert, Robert M.","Gregory, Eugene M."],"year":1988,"date_issued":"1988-12-05","date_published":"1988-12-05","updated_at":"2026-07-22T22:18:39Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11212012-040236"],"render_values":[{"text":"etd-11212012-040236","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/45965","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Krieg, Noel R."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Smibert, Robert M.","Gregory, Eugene M."]},{"key":"dc:contributor.department","label":"Department","values":["Microbiology"]},{"key":"dc:creator","label":"Author","values":["Vercellone, Pamela Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:50:28Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:50:28Z","2012-11-21"]},{"key":"dc:date.issued","label":"Date","values":["1988-12-05"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11212012-040236"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/45965"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A comparative study of the microaerophilic <u>Campylobacter jejuni</u> strain H840 with an aerotolerant mutant, MC711-01, revealed that catalase and, to a lesser extent, SOD activity is correlated with the aerotolerance and enhanced resistance of MC7ll-0l to H₂O₂. When cells were cultured under 6% oxygen, the specific activity of catalase was significantly higher in crude extracts of MC711-01 than of H840. The catalase activity of MC711-01 more than doubled when cells were cultured under 21% oxygen, and this activity was 2.6 times greater than that of H84O; no corresponding increase was observed in strain H840. There was no significant difference in the mean SOD activity of the two strains when cultured under 6% oxygen or in H840 cells cultured under either 6% or 21% oxygen; however, the SOD activity of MC711-01 increased 1.5 times when cells were cultured under 21% oxygen. Survival studies revealed that MC711-01 was significantly more E resistant to H₂O₂ when cultured under either 6% or 21% oxygen. However, both MC711-01 and H84O were more susceptible to H₂O₂ when grown under 21% oxygen, indicating that both strains might be more highly stressed when the cells are grown at this oxygen tension. The present study suggests that in <u>C. jejuni</u>, the level of catalase activity may influence the degree of susceptibility to H₂O₂ and consequently, the degree of aerotolerance."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Oxygen toxicity in Campylobacter jejuni: physiological comparison of a microaerophilic wild-type strain with an aerotolerant mutant"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Krieg, Noel R."],"dc:contributor.committeemember":["Smibert, Robert M.","Gregory, Eugene M."],"dc:contributor.department":["Microbiology"],"dc:creator":["Vercellone, Pamela Ann"],"dc:date.accessioned":["2014-03-14T21:50:28Z"],"dc:date.available":["2014-03-14T21:50:28Z","2012-11-21"],"dc:date.issued":["1988-12-05"],"dc:description.abstract":["A comparative study of the microaerophilic <u>Campylobacter jejuni</u> strain H840 with an aerotolerant mutant, MC711-01, revealed that catalase and, to a lesser extent, SOD activity is correlated with the aerotolerance and enhanced resistance of MC7ll-0l to H₂O₂. When cells were cultured under 6% oxygen, the specific activity of catalase was significantly higher in crude extracts of MC711-01 than of H840. The catalase activity of MC711-01 more than doubled when cells were cultured under 21% oxygen, and this activity was 2.6 times greater than that of H84O; no corresponding increase was observed in strain H840. There was no significant difference in the mean SOD activity of the two strains when cultured under 6% oxygen or in H840 cells cultured under either 6% or 21% oxygen; however, the SOD activity of MC711-01 increased 1.5 times when cells were cultured under 21% oxygen. Survival studies revealed that MC711-01 was significantly more E resistant to H₂O₂ when cultured under either 6% or 21% oxygen. However, both MC711-01 and H84O were more susceptible to H₂O₂ when grown under 21% oxygen, indicating that both strains might be more highly stressed when the cells are grown at this oxygen tension. The present study suggests that in <u>C. jejuni</u>, the level of catalase activity may influence the degree of susceptibility to H₂O₂ and consequently, the degree of aerotolerance."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-11212012-040236"],"dc:identifier.uri":["http://hdl.handle.net/10919/45965"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Oxygen toxicity in Campylobacter jejuni: physiological comparison of a microaerophilic wild-type strain with an aerotolerant mutant"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:18:39Z"}