{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/45463"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/45463","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The 16S rRNA characterization of a novel \"microaerophilic\" Pseudomonas sp. from the oligotrophic deep subsurface environment","abstract":"A gram negative microaerophilic bacterium, designated Pseudomonas sp. strain MR 100, was isolated from a depth of 463 meters at the Savannah River DOE site and identified using 16S rDNA sequencing and DNA-DNA reassociation. Micro aerophiles from the Middendorf formation were isolated by use of a semi-solid agar assay, and constituted 10% of the plateable microorganisms. Genetic identification involved the isolation of genomic DNA and amplification of the gene encoding 16S rRNA by PCR, using universal primers. The amplified DNA was sequenced and compared to 16S rRNA sequences in Genbank. High sequence similarity (98.5%) was observed with the <i>Pseudomonas mendocina</i> type strain, indicating a similarity to the (Group I) pseudomonads. DNA-DNA reassociation was performed between <i>Pseudomonas</i> sp. strain MR 100 and 11 representative p seudomonads using the S 1 nuclease method. Strain MR 100 was found to be 20% homologous to the <i>Pseudomonas mendocina</i> type strain, 10% homologous to <i>Pseudomonas alcaligenes</i>, and 5% homologous to <i>Pseudomonas aeruginosa</i>. Data from biochemical tests confirm the hypothesis that strain MR 100 is a novel species of <i>Pseudomonas</I. It was able to accumulate poly-β-hydroxybutyrate intracellularly, while it lacked the ability to produce cellular pigments, which is unique among the (Group I) pseudomonads. Growth occurred at oxygen concentrations of 20/0 and 21%, with similar growth rates and final cell densities.","abstract_html":"A gram negative microaerophilic bacterium, designated Pseudomonas sp. strain MR 100, was isolated from a depth of 463 meters at the Savannah River DOE site and identified using 16S rDNA sequencing and DNA-DNA reassociation. Micro aerophiles from the Middendorf formation were isolated by use of a semi-solid agar assay, and constituted 10% of the plateable microorganisms. Genetic identification involved the isolation of genomic DNA and amplification of the gene encoding 16S rRNA by PCR, using universal primers. The amplified DNA was sequenced and compared to 16S rRNA sequences in Genbank. High sequence similarity (98.5%) was observed with the &lt;i&gt;Pseudomonas mendocina&lt;/i&gt; type strain, indicating a similarity to the (Group I) pseudomonads. DNA-DNA reassociation was performed between &lt;i&gt;Pseudomonas&lt;/i&gt; sp. strain MR 100 and 11 representative p seudomonads using the S 1 nuclease method. Strain MR 100 was found to be 20% homologous to the &lt;i&gt;Pseudomonas mendocina&lt;/i&gt; type strain, 10% homologous to &lt;i&gt;Pseudomonas alcaligenes&lt;/i&gt;, and 5% homologous to &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;. Data from biochemical tests confirm the hypothesis that strain MR 100 is a novel species of &lt;i&gt;Pseudomonas&lt;/I. It was able to accumulate poly-β-hydroxybutyrate intracellularly, while it lacked the ability to produce cellular pigments, which is unique among the (Group I) pseudomonads. Growth occurred at oxygen concentrations of 20/0 and 21%, with similar growth rates and final cell densities.","abstract_has_math":false,"creators":["Lampe, Robert Carl III"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Microbiology","degree_department":"Microbiology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Benoit, Robert E."],"committee_members":["Yousten, Allan A.","Krieg, Noel R."],"year":1996,"date_issued":"1996-11-07","date_published":"1996-11-07","updated_at":"2026-07-22T22:19:00Z","subjects":["DNA reassocation","16S rRNA","microaerophile","subsurface","Pseudomonas"],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11072008-063340"],"render_values":[{"text":"etd-11072008-063340","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/45463","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Benoit, Robert E."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Yousten, Allan A.","Krieg, Noel R."]},{"key":"dc:contributor.department","label":"Department","values":["Microbiology"]},{"key":"dc:creator","label":"Author","values":["Lampe, Robert Carl III"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:49:03Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:49:03Z","2008-11-07"]},{"key":"dc:date.issued","label":"Date","values":["1996-11-07"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["DNA reassocation","16S rRNA","microaerophile","subsurface","Pseudomonas"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-11072008-063340"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/45463"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A gram negative microaerophilic bacterium, designated Pseudomonas sp. strain MR 100, was isolated from a depth of 463 meters at the Savannah River DOE site and identified using 16S rDNA sequencing and DNA-DNA reassociation. Micro aerophiles from the Middendorf formation were isolated by use of a semi-solid agar assay, and constituted 10% of the plateable microorganisms. Genetic identification involved the isolation of genomic DNA and amplification of the gene encoding 16S rRNA by PCR, using universal primers. The amplified DNA was sequenced and compared to 16S rRNA sequences in Genbank. High sequence similarity (98.5%) was observed with the <i>Pseudomonas mendocina</i> type strain, indicating a similarity to the (Group I) pseudomonads. DNA-DNA reassociation was performed between <i>Pseudomonas</i> sp. strain MR 100 and 11 representative p seudomonads using the S 1 nuclease method. Strain MR 100 was found to be 20% homologous to the <i>Pseudomonas mendocina</i> type strain, 10% homologous to <i>Pseudomonas alcaligenes</i>, and 5% homologous to <i>Pseudomonas aeruginosa</i>. Data from biochemical tests confirm the hypothesis that strain MR 100 is a novel species of <i>Pseudomonas</I. It was able to accumulate poly-β-hydroxybutyrate intracellularly, while it lacked the ability to produce cellular pigments, which is unique among the (Group I) pseudomonads. Growth occurred at oxygen concentrations of 20/0 and 21%, with similar growth rates and final cell densities."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The 16S rRNA characterization of a novel \"microaerophilic\" Pseudomonas sp. from the oligotrophic deep subsurface environment"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Benoit, Robert E."],"dc:contributor.committeemember":["Yousten, Allan A.","Krieg, Noel R."],"dc:contributor.department":["Microbiology"],"dc:creator":["Lampe, Robert Carl III"],"dc:date.accessioned":["2014-03-14T21:49:03Z"],"dc:date.available":["2014-03-14T21:49:03Z","2008-11-07"],"dc:date.issued":["1996-11-07"],"dc:description.abstract":["A gram negative microaerophilic bacterium, designated Pseudomonas sp. strain MR 100, was isolated from a depth of 463 meters at the Savannah River DOE site and identified using 16S rDNA sequencing and DNA-DNA reassociation. Micro aerophiles from the Middendorf formation were isolated by use of a semi-solid agar assay, and constituted 10% of the plateable microorganisms. Genetic identification involved the isolation of genomic DNA and amplification of the gene encoding 16S rRNA by PCR, using universal primers. The amplified DNA was sequenced and compared to 16S rRNA sequences in Genbank. High sequence similarity (98.5%) was observed with the <i>Pseudomonas mendocina</i> type strain, indicating a similarity to the (Group I) pseudomonads. DNA-DNA reassociation was performed between <i>Pseudomonas</i> sp. strain MR 100 and 11 representative p seudomonads using the S 1 nuclease method. Strain MR 100 was found to be 20% homologous to the <i>Pseudomonas mendocina</i> type strain, 10% homologous to <i>Pseudomonas alcaligenes</i>, and 5% homologous to <i>Pseudomonas aeruginosa</i>. Data from biochemical tests confirm the hypothesis that strain MR 100 is a novel species of <i>Pseudomonas</I. It was able to accumulate poly-β-hydroxybutyrate intracellularly, while it lacked the ability to produce cellular pigments, which is unique among the (Group I) pseudomonads. Growth occurred at oxygen concentrations of 20/0 and 21%, with similar growth rates and final cell densities."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-11072008-063340"],"dc:identifier.uri":["http://hdl.handle.net/10919/45463"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["DNA reassocation","16S rRNA","microaerophile","subsurface","Pseudomonas"],"dc:title":["The 16S rRNA characterization of a novel \"microaerophilic\" Pseudomonas sp. from the oligotrophic deep subsurface environment"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:00Z"}