{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/45106"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/45106","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Metabolism, nutritional effects, and mutagenesis of crystal violet decolorization by a biofungicide agent Pseudomonas putida strain M-17","abstract":"A strain of Pseudomonas putida that exhibited seedling disease control on cotton was among 5000 strains examined for unique properties that could be used to selectively recover genetically engineered pseudomonads from environmental samples. One isolate (M-17) was found to produce a red halo around single colonies grown on media containing 10 mg/l crystal violet. This decolorization reaction was constitutively produced when the growth medium contained glucose and asparagine, but was inhibited by the substitution of ammonia, nitrate or urea for the amino acid nitrogen source. However, a different medium containing succinic acid and using ammonia as the sole nitrogen source was found to induce the reaction. Factors that did not affect the decolorization reaction included temperature (14-30° C) and pH (3-8). Cultures of M-17 grown in broth containing crystal violet were able to decolorize still broth solutions, but not when incubated on a shaker (150 rpm). Stationary cultures formed a red precipitate. Efforts to characterize the precipitate revealed that it was soluble in polar organic solvents and insoluble in non-polar organic solvents. Thin layer chromatography revealed the presence of six bands that possibly represented various demethylated forms of crystal violet. Chemically derived mutants (cry-) did not produce a red halo but a clear, colorless region surrounding bacterial growth was observed. A greenhouse study demonstrated that strain M-17 provided protection against fungal disease as shown by plant stands on cotton (67% stand) equivalent to the commercial fungicides (70% stand) and significantly improved over the unamended control (38% stand).","abstract_html":"A strain of Pseudomonas putida that exhibited seedling disease control on cotton was among 5000 strains examined for unique properties that could be used to selectively recover genetically engineered pseudomonads from environmental samples. One isolate (M-17) was found to produce a red halo around single colonies grown on media containing 10 mg/l crystal violet. This decolorization reaction was constitutively produced when the growth medium contained glucose and asparagine, but was inhibited by the substitution of ammonia, nitrate or urea for the amino acid nitrogen source. However, a different medium containing succinic acid and using ammonia as the sole nitrogen source was found to induce the reaction. Factors that did not affect the decolorization reaction included temperature (14-30° C) and pH (3-8). Cultures of M-17 grown in broth containing crystal violet were able to decolorize still broth solutions, but not when incubated on a shaker (150 rpm). Stationary cultures formed a red precipitate. Efforts to characterize the precipitate revealed that it was soluble in polar organic solvents and insoluble in non-polar organic solvents. Thin layer chromatography revealed the presence of six bands that possibly represented various demethylated forms of crystal violet. Chemically derived mutants (cry-) did not produce a red halo but a clear, colorless region surrounding bacterial growth was observed. A greenhouse study demonstrated that strain M-17 provided protection against fungal disease as shown by plant stands on cotton (67% stand) equivalent to the commercial fungicides (70% stand) and significantly improved over the unamended control (38% stand).","abstract_has_math":false,"creators":["McCuistion, Fred Talmadge"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Crop and Soil Environmental Sciences","degree_department":"Crop and Soil Environmental Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1991,"date_issued":"1991","date_published":"1991","updated_at":"2026-07-22T22:20:09Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10102009-020058"],"render_values":[{"text":"etd-10102009-020058","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/45106","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.department","label":"Department","values":["Crop and Soil Environmental Sciences"]},{"key":"dc:creator","label":"Author","values":["McCuistion, Fred Talmadge"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:47:12Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:47:12Z","2009-10-10"]},{"key":"dc:date.issued","label":"Date","values":["1991"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Crop and Soil Environmental Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10102009-020058"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/45106"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A strain of Pseudomonas putida that exhibited seedling disease control on cotton was among 5000 strains examined for unique properties that could be used to selectively recover genetically engineered pseudomonads from environmental samples. One isolate (M-17) was found to produce a red halo around single colonies grown on media containing 10 mg/l crystal violet. This decolorization reaction was constitutively produced when the growth medium contained glucose and asparagine, but was inhibited by the substitution of ammonia, nitrate or urea for the amino acid nitrogen source. However, a different medium containing succinic acid and using ammonia as the sole nitrogen source was found to induce the reaction. Factors that did not affect the decolorization reaction included temperature (14-30° C) and pH (3-8). Cultures of M-17 grown in broth containing crystal violet were able to decolorize still broth solutions, but not when incubated on a shaker (150 rpm). Stationary cultures formed a red precipitate. Efforts to characterize the precipitate revealed that it was soluble in polar organic solvents and insoluble in non-polar organic solvents. Thin layer chromatography revealed the presence of six bands that possibly represented various demethylated forms of crystal violet. Chemically derived mutants (cry-) did not produce a red halo but a clear, colorless region surrounding bacterial growth was observed. A greenhouse study demonstrated that strain M-17 provided protection against fungal disease as shown by plant stands on cotton (67% stand) equivalent to the commercial fungicides (70% stand) and significantly improved over the unamended control (38% stand)."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Metabolism, nutritional effects, and mutagenesis of crystal violet decolorization by a biofungicide agent Pseudomonas putida strain M-17"]}]}],"canonical_facts":{"dc:contributor.department":["Crop and Soil Environmental Sciences"],"dc:creator":["McCuistion, Fred Talmadge"],"dc:date.accessioned":["2014-03-14T21:47:12Z"],"dc:date.available":["2014-03-14T21:47:12Z","2009-10-10"],"dc:date.issued":["1991"],"dc:description.abstract":["A strain of Pseudomonas putida that exhibited seedling disease control on cotton was among 5000 strains examined for unique properties that could be used to selectively recover genetically engineered pseudomonads from environmental samples. One isolate (M-17) was found to produce a red halo around single colonies grown on media containing 10 mg/l crystal violet. This decolorization reaction was constitutively produced when the growth medium contained glucose and asparagine, but was inhibited by the substitution of ammonia, nitrate or urea for the amino acid nitrogen source. However, a different medium containing succinic acid and using ammonia as the sole nitrogen source was found to induce the reaction. Factors that did not affect the decolorization reaction included temperature (14-30° C) and pH (3-8). Cultures of M-17 grown in broth containing crystal violet were able to decolorize still broth solutions, but not when incubated on a shaker (150 rpm). Stationary cultures formed a red precipitate. Efforts to characterize the precipitate revealed that it was soluble in polar organic solvents and insoluble in non-polar organic solvents. Thin layer chromatography revealed the presence of six bands that possibly represented various demethylated forms of crystal violet. Chemically derived mutants (cry-) did not produce a red halo but a clear, colorless region surrounding bacterial growth was observed. A greenhouse study demonstrated that strain M-17 provided protection against fungal disease as shown by plant stands on cotton (67% stand) equivalent to the commercial fungicides (70% stand) and significantly improved over the unamended control (38% stand)."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-10102009-020058"],"dc:identifier.uri":["http://hdl.handle.net/10919/45106"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Metabolism, nutritional effects, and mutagenesis of crystal violet decolorization by a biofungicide agent Pseudomonas putida strain M-17"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Crop and Soil Environmental Sciences"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:09Z"}