{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/44831"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/44831","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Epidermal growth factor receptor in equine gastric stratified squamous mucosa: effect of progressive ulceration on receptor density","abstract":"The objective of the study reported here was to document the distribution of epidermal growth factor receptor (EGFr) and quantitate receptor density in normal as well as ulcerated equine gastric squamous mucosa. Fifteen horses with endoscopically normal stomachs were divided into three equal groups. Group 1 was a normal control. A protocol that alternated 24 hour periods of free-choice hay with 24 hours of feed deprivation was utilized to induce squamous mucosal gastric ulceration in Group 2 (48 hours total off-feed) and Group 3 (96 hours total off-feed). Gastric tissue was collected from 3 stomach locations at post-mortem examination and an avidin-biotin immunoperoxidase technique was developed to stain the formalin-fixed tissue for EGFr. A computerized image analysis system was used to measure EGFr area and mean intensity values at four sites within the epithelium from the basal cell layers to the lumen in the ulcer/erosion margin, erosion bed, and 10-14 mm distant from the lesion.","abstract_html":"The objective of the study reported here was to document the distribution of epidermal growth factor receptor (EGFr) and quantitate receptor density in normal as well as ulcerated equine gastric squamous mucosa. Fifteen horses with endoscopically normal stomachs were divided into three equal groups. Group 1 was a normal control. A protocol that alternated 24 hour periods of free-choice hay with 24 hours of feed deprivation was utilized to induce squamous mucosal gastric ulceration in Group 2 (48 hours total off-feed) and Group 3 (96 hours total off-feed). Gastric tissue was collected from 3 stomach locations at post-mortem examination and an avidin-biotin immunoperoxidase technique was developed to stain the formalin-fixed tissue for EGFr. A computerized image analysis system was used to measure EGFr area and mean intensity values at four sites within the epithelium from the basal cell layers to the lumen in the ulcer/erosion margin, erosion bed, and 10-14 mm distant from the lesion.","abstract_has_math":false,"creators":["Jeffrey, Stuart C."],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Veterinary Medical Sciences","degree_department":"Veterinary Medical Sciences","school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":["Murray, Michael J.","Pfeiffer, Carl J.","Robertson, John L."],"year":1996,"date_issued":"1996-02-05","date_published":"1996-02-05","updated_at":"2026-07-22T22:19:10Z","subjects":["gastric ulcers","epidermal growth factor receptor","equine"],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-09182008-063547"],"render_values":[{"text":"etd-09182008-063547","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/44831","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Murray, Michael J.","Pfeiffer, Carl J.","Robertson, John L."]},{"key":"dc:contributor.department","label":"Department","values":["Veterinary Medical Sciences"]},{"key":"dc:creator","label":"Author","values":["Jeffrey, Stuart C."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:46:04Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:46:04Z","2008-09-18"]},{"key":"dc:date.issued","label":"Date","values":["1996-02-05"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Medical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["gastric ulcers","epidermal growth factor receptor","equine"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-09182008-063547"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/44831"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The objective of the study reported here was to document the distribution of epidermal growth factor receptor (EGFr) and quantitate receptor density in normal as well as ulcerated equine gastric squamous mucosa. 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