Analysis of Campylobacter jejuni, Campylobacter coli, Salmonella, Klebsiella pneumoniae, and Escherichia coli 0157:H7 in fresh hand picked blue crab (Callinectes sapidus) meat

This study was conducted to determine the quantities of Campylobacter jejuni, Campylobacter coli, Salmonella, fecal positive Klebsiella pneumoniae, and Escherichia coli 0157:H7 in fresh hand picked blue crab (Callinectes sapidus) meat. An attempt was made to correlate these selected pathogens and general microbial quality to processors Sanitation practices and facility size. Hand picked crabmeat samples from 12 blue crab processing facilities in the Chesapeake Bay region were collected and analyzed. Twenty samples from each of the different facilities were collected on different processing days. Facilities were chosen based on production levels and Virginia Department of Health inspection scores as an indicator of sanitation practices (excellent ≥ 94.5, acceptable < 94.5). All samples were tested between 16 h and 36 h after collection. Campylobacter jejuni was isolated from 36 (15%) of the 240 samples and Campylobacter coli was isolated from 14 (5.8%). Quantitative levels in all case were below limits of detection (<0.30 MPN/g). Klebsiella pneumoniae was isolated from a total of 51 (21%) samples. Counts ranged from less then 0.30 to 4.3 MPN/g. Aerobic plate counts ranged from 7.4x10³ to 4.6x10⁸ CFU/g with coliform counts ranging from <0.3 to 32.8 MPN/g. Fecal coliform levels were <0.3 to 2.26 MPN/g and Escherichia coli from <0.3 to 0.77 MPN/g. Salmonella and Escherichia coli 0157:H7 were not detected in any of the 240 samples analyzed. No significant differences (p<0.05) between size and inspection scores were observed for general microbial quality, the presence of Klebsiella pneumoniae, or Campylobacter species.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Food Science and Technology

Department dc:contributor.department

Food Science and Technology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1995

Author dc:creator

• Reinhard, Robert G.

• seafood
• crabmeat

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-08142009-040632

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/44250