{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/43237"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/43237","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The distribution of anaerobic bacteria along a soil drainage catena","abstract":"Strict anaerobic culture techniques were used to enumerate the anaerobic bacteria present in three soil sites located along a drainage catena near Blacksburg, Virginia. An anaerobic cooked meat plus 0.5% glucose medium cultured the largest number of anaerobes from the poorly drained soil. The population of obligate anaerobes ranged from 10⁶/ g dry weight soil on the poorly drained soil (% moisture = 112.06) to 10⁵/g dry weight soil on the intermediate soil (% moisture = 34.51) to 10⁴/g dry weight soil on the well drained soil (% moisture 20.81). The population of organisms able to grow anaerobically (facultative plus obligate) ranged from 10⁶/g dry weight soil on the poorly drained site to 10⁵/g dry weight soil on the well drained site. This same population on the poorly drained site was relatively constant over a nine month period with the exception of a sharp rise in early spring. The clostridia constituted at least one third of the obligately anaerobic bacteria present on the poorly drained soil. A sizeable percentage of the obligate anaerobic isolates on this site were either clostridia which formed spores unable to germinate in the medium employed, clostridia which were very pleomorphic in cell shape and gram reaction, or nonsporeforming obligate anaerobes. These results indicate that strict anaerobes and possibly nonsporeforming strict anaerobes exist in soils of different drainage character even though facultative organisms appear to be more successful competitors on the more well drained sites.","abstract_html":"Strict anaerobic culture techniques were used to enumerate the anaerobic bacteria present in three soil sites located along a drainage catena near Blacksburg, Virginia. An anaerobic cooked meat plus 0.5% glucose medium cultured the largest number of anaerobes from the poorly drained soil. The population of obligate anaerobes ranged from 10⁶/ g dry weight soil on the poorly drained soil (% moisture = 112.06) to 10⁵/g dry weight soil on the intermediate soil (% moisture = 34.51) to 10⁴/g dry weight soil on the well drained soil (% moisture 20.81). The population of organisms able to grow anaerobically (facultative plus obligate) ranged from 10⁶/g dry weight soil on the poorly drained site to 10⁵/g dry weight soil on the well drained site. This same population on the poorly drained site was relatively constant over a nine month period with the exception of a sharp rise in early spring. The clostridia constituted at least one third of the obligately anaerobic bacteria present on the poorly drained soil. A sizeable percentage of the obligate anaerobic isolates on this site were either clostridia which formed spores unable to germinate in the medium employed, clostridia which were very pleomorphic in cell shape and gram reaction, or nonsporeforming obligate anaerobes. These results indicate that strict anaerobes and possibly nonsporeforming strict anaerobes exist in soils of different drainage character even though facultative organisms appear to be more successful competitors on the more well drained sites.","abstract_has_math":false,"creators":["Dolan, Rodney Martin"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Microbiology","degree_department":"Microbiology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Benoit, Robert E."],"committee_members":["Holdeman, Lillian V.","Martens, David C."],"year":1977,"date_issued":"1977-03-05","date_published":"1977-03-05","updated_at":"2026-07-22T22:19:45Z","subjects":[],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06122010-020220"],"render_values":[{"text":"etd-06122010-020220","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/43237","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Benoit, Robert E."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Holdeman, Lillian V.","Martens, David C."]},{"key":"dc:contributor.department","label":"Department","values":["Microbiology"]},{"key":"dc:creator","label":"Author","values":["Dolan, Rodney Martin"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:38:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:38:20Z","2010-06-12"]},{"key":"dc:date.issued","label":"Date","values":["1977-03-05"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06122010-020220"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/43237"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Strict anaerobic culture techniques were used to enumerate the anaerobic bacteria present in three soil sites located along a drainage catena near Blacksburg, Virginia. An anaerobic cooked meat plus 0.5% glucose medium cultured the largest number of anaerobes from the poorly drained soil. The population of obligate anaerobes ranged from 10⁶/ g dry weight soil on the poorly drained soil (% moisture = 112.06) to 10⁵/g dry weight soil on the intermediate soil (% moisture = 34.51) to 10⁴/g dry weight soil on the well drained soil (% moisture 20.81). The population of organisms able to grow anaerobically (facultative plus obligate) ranged from 10⁶/g dry weight soil on the poorly drained site to 10⁵/g dry weight soil on the well drained site. This same population on the poorly drained site was relatively constant over a nine month period with the exception of a sharp rise in early spring. The clostridia constituted at least one third of the obligately anaerobic bacteria present on the poorly drained soil. A sizeable percentage of the obligate anaerobic isolates on this site were either clostridia which formed spores unable to germinate in the medium employed, clostridia which were very pleomorphic in cell shape and gram reaction, or nonsporeforming obligate anaerobes. These results indicate that strict anaerobes and possibly nonsporeforming strict anaerobes exist in soils of different drainage character even though facultative organisms appear to be more successful competitors on the more well drained sites."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The distribution of anaerobic bacteria along a soil drainage catena"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Benoit, Robert E."],"dc:contributor.committeemember":["Holdeman, Lillian V.","Martens, David C."],"dc:contributor.department":["Microbiology"],"dc:creator":["Dolan, Rodney Martin"],"dc:date.accessioned":["2014-03-14T21:38:20Z"],"dc:date.available":["2014-03-14T21:38:20Z","2010-06-12"],"dc:date.issued":["1977-03-05"],"dc:description.abstract":["Strict anaerobic culture techniques were used to enumerate the anaerobic bacteria present in three soil sites located along a drainage catena near Blacksburg, Virginia. An anaerobic cooked meat plus 0.5% glucose medium cultured the largest number of anaerobes from the poorly drained soil. The population of obligate anaerobes ranged from 10⁶/ g dry weight soil on the poorly drained soil (% moisture = 112.06) to 10⁵/g dry weight soil on the intermediate soil (% moisture = 34.51) to 10⁴/g dry weight soil on the well drained soil (% moisture 20.81). The population of organisms able to grow anaerobically (facultative plus obligate) ranged from 10⁶/g dry weight soil on the poorly drained site to 10⁵/g dry weight soil on the well drained site. This same population on the poorly drained site was relatively constant over a nine month period with the exception of a sharp rise in early spring. The clostridia constituted at least one third of the obligately anaerobic bacteria present on the poorly drained soil. A sizeable percentage of the obligate anaerobic isolates on this site were either clostridia which formed spores unable to germinate in the medium employed, clostridia which were very pleomorphic in cell shape and gram reaction, or nonsporeforming obligate anaerobes. These results indicate that strict anaerobes and possibly nonsporeforming strict anaerobes exist in soils of different drainage character even though facultative organisms appear to be more successful competitors on the more well drained sites."],"dc:description.degree":["Master of Science"],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-06122010-020220"],"dc:identifier.uri":["http://hdl.handle.net/10919/43237"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["The distribution of anaerobic bacteria along a soil drainage catena"],"dc:type":["Thesis"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:19:45Z"}