{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/43054"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/43054","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Evaluation of a mitochondrial test for the determination of chemical toxicity","abstract":"The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. Mitochondrial fractions were exposed to six concentrations of five chlorophenols. Respiratory parameters were measured and compared to control experiments. The toxicity of the chlorophenos, measured by the 50% uncoupling concentration (UC50), increased with increasing chloro substitution. The UC50 values for the five chlorophenols were compared to six physicochemical parameters for the same chlorophenols and high degrees of correlation were found (r >/- 0.890). The highest correlation coefficient obtained was with the octanol-water partition coefficient. UC50 values were also compared to nine currently existing short-term toxicity tests. High degrees of correlation were obtained with several of these tests, including bacterial and fish bioassays. From the results of these experiments, the measurement and use of mitochondria respiratory parameters as a short-term toxicity test appears to offer an alternative to currently used short-term toxicity tests, particularly with chemicals having physicochemical characteristics similar to mitochondria uncouplers.","abstract_html":"The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. Mitochondrial fractions were exposed to six concentrations of five chlorophenols. Respiratory parameters were measured and compared to control experiments. The toxicity of the chlorophenos, measured by the 50% uncoupling concentration (UC50), increased with increasing chloro substitution. The UC50 values for the five chlorophenols were compared to six physicochemical parameters for the same chlorophenols and high degrees of correlation were found (r >/- 0.890). The highest correlation coefficient obtained was with the octanol-water partition coefficient. UC50 values were also compared to nine currently existing short-term toxicity tests. High degrees of correlation were obtained with several of these tests, including bacterial and fish bioassays. From the results of these experiments, the measurement and use of mitochondria respiratory parameters as a short-term toxicity test appears to offer an alternative to currently used short-term toxicity tests, particularly with chemicals having physicochemical characteristics similar to mitochondria uncouplers.","abstract_has_math":false,"creators":["Shannon, Robert David"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Environmental Sciences and Engineering","degree_department":"Environmental Sciences and Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Boardman, Gregory D."],"committee_members":["Hoehn, Robert C.","Bevan, David R.","Dietrich, Andrea M."],"year":1988,"date_issued":"1988-07-15","date_published":"1988-07-15","updated_at":"2026-07-22T22:20:21Z","subjects":[],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06102012-040255"],"render_values":[{"text":"etd-06102012-040255","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/43054","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Boardman, Gregory D."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Hoehn, Robert C.","Bevan, David R.","Dietrich, Andrea M."]},{"key":"dc:contributor.department","label":"Department","values":["Environmental Sciences and Engineering"]},{"key":"dc:creator","label":"Author","values":["Shannon, Robert David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:37:41Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:37:41Z","2012-06-10"]},{"key":"dc:date.issued","label":"Date","values":["1988-07-15"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Environmental Sciences and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06102012-040255"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/43054"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. 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From the results of these experiments, the measurement and use of mitochondria respiratory parameters as a short-term toxicity test appears to offer an alternative to currently used short-term toxicity tests, particularly with chemicals having physicochemical characteristics similar to mitochondria uncouplers."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Evaluation of a mitochondrial test for the determination of chemical toxicity"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Boardman, Gregory D."],"dc:contributor.committeemember":["Hoehn, Robert C.","Bevan, David R.","Dietrich, Andrea M."],"dc:contributor.department":["Environmental Sciences and Engineering"],"dc:creator":["Shannon, Robert David"],"dc:date.accessioned":["2014-03-14T21:37:41Z"],"dc:date.available":["2014-03-14T21:37:41Z","2012-06-10"],"dc:date.issued":["1988-07-15"],"dc:description.abstract":["The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. 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