{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/42578"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/42578","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"The Design of Polyelectrolyte Multilayers Using Galactosylated Chitosan","abstract":"A major challenge in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. Hepatocytes have a surface protein, called the asialoglycoprotein receptor (ASGP-R), which interacts with galactose via a specific receptor-ligand interaction. Polyelectrolyte multilayers (PEMs) were prepared by the layer by layer method, which is based on electrostatic attractions between oppositely charged polyelectrolytes (PEs). Anionic (hyaluronic acid) and cationic (chitosan and galactosylated chitosan) PEs were used in the fabrication of detachable, free-standing PEMs. The main focus of this study is the design of PEMs comprised of 50 bilayers of PEs. PEMs that contained galactose functional groups were assembled with either 5 or 10 bilayers of galactosylated chitosan (5 - 10 % of galactosylation). Optical properties, solvent stability and surface topography of the PEMs were measured.","abstract_html":"A major challenge in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. Hepatocytes have a surface protein, called the asialoglycoprotein receptor (ASGP-R), which interacts with galactose via a specific receptor-ligand interaction. Polyelectrolyte multilayers (PEMs) were prepared by the layer by layer method, which is based on electrostatic attractions between oppositely charged polyelectrolytes (PEs). Anionic (hyaluronic acid) and cationic (chitosan and galactosylated chitosan) PEs were used in the fabrication of detachable, free-standing PEMs. The main focus of this study is the design of PEMs comprised of 50 bilayers of PEs. PEMs that contained galactose functional groups were assembled with either 5 or 10 bilayers of galactosylated chitosan (5 - 10 % of galactosylation). Optical properties, solvent stability and surface topography of the PEMs were measured.","abstract_has_math":false,"creators":["Arca, Hale Cigdem"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Macromolecular Science and Engineering","degree_department":"Macromolecular Science and Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Rajagopalan, Padmavathy"],"committee_members":["Davis, Richey M.","Edgar, Kevin J."],"year":2012,"date_issued":"2012-04-26","date_published":"2012-04-26","updated_at":"2026-07-22T22:19:01Z","subjects":["ASGP-R","Chitosan","Galactose","Liver models","Polyelectrolyte multilayers"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-05092012-004123"],"render_values":[{"text":"etd-05092012-004123","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/42578","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Rajagopalan, Padmavathy"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Davis, Richey M.","Edgar, Kevin J."]},{"key":"dc:contributor.department","label":"Department","values":["Macromolecular Science and Engineering"]},{"key":"dc:creator","label":"Author","values":["Arca, Hale Cigdem"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:35:53Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:35:53Z","2012-06-15"]},{"key":"dc:date.issued","label":"Date","values":["2012-04-26"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Macromolecular Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["ASGP-R","Chitosan","Galactose","Liver models","Polyelectrolyte multilayers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-05092012-004123"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/42578"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A major challenge in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. Hepatocytes have a surface protein, called the asialoglycoprotein receptor (ASGP-R), which interacts with galactose via a specific receptor-ligand interaction. Polyelectrolyte multilayers (PEMs) were prepared by the layer by layer method, which is based on electrostatic attractions between oppositely charged polyelectrolytes (PEs). Anionic (hyaluronic acid) and cationic (chitosan and galactosylated chitosan) PEs were used in the fabrication of detachable, free-standing PEMs. The main focus of this study is the design of PEMs comprised of 50 bilayers of PEs. PEMs that contained galactose functional groups were assembled with either 5 or 10 bilayers of galactosylated chitosan (5 - 10 % of galactosylation). Optical properties, solvent stability and surface topography of the PEMs were measured."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["The Design of Polyelectrolyte Multilayers Using Galactosylated Chitosan"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Rajagopalan, Padmavathy"],"dc:contributor.committeemember":["Davis, Richey M.","Edgar, Kevin J."],"dc:contributor.department":["Macromolecular Science and Engineering"],"dc:creator":["Arca, Hale Cigdem"],"dc:date.accessioned":["2014-03-14T21:35:53Z"],"dc:date.available":["2014-03-14T21:35:53Z","2012-06-15"],"dc:date.issued":["2012-04-26"],"dc:description.abstract":["A major challenge in hepatic tissue engineering is that liver cells rapidly lose their phenotype in in vitro cell culture systems. For this reason, it is necessary to design biomaterials that can support and enhance hepatic functions. 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