Effect of krilium on the respiratory activities of Rhizobium trifolii and Agrobacterium tumefaciens on various substrates

The respiratory rates of Rhizobium trifolii and Agrobacterium tumefaciens were measured by use of a Warburg Respirometer. A non-proliferating or "resting cell" technique was utilized in demonstrating the effect of Krilium on the oxygen consumption of the organisms when Krilium was aided to a given substrate, glucose, succinate or mannitol. Two types of Krilium were used in this investigation, Krilium (Blend #6) and Krilium (Blend #9). Observations were made on the effect of various concentrations (.05%, .10% and 1.0%) of the two blends of Krilium on the respiratory activities of the test organisms. It was observed that Krillium (Blend #6) caused a noticeable increase in the respiratory rate of Rhizobium trifolii, whereas in the case of Agrobacterium tumefaciens, no effect or a slight decrease was observed when Krilium (Blend #9) was added to the substrate, A very noticeable decreases in respiratory rates was observed when both test organisms were permitted to respire on substrates containing Krilium (Blend #9). When Krilium (Blend #6) and Krilium (Blend #9) were used as the only substrates, an increase in respiration rates of the organisms was observed. It is indicative from this work that Krilium (Blend #6) either causes a slight increase In the respiratory activities or has no deleterious effect on the respiration of the test organisms., The results obtained, when using Krilium (Blend #9), seem to indicate that this blend of Krillum has an undesirable effect on the respiration of the two organisms used in this investigation.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biochemistry

Department dc:contributor.department

Biochemistry

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1953

Author dc:creator

• Stafford, W. R.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-04072010-020017

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/41952