Effect of immunoglobulins on early bovine embryo development in vitro

Bovine morulae (day 6: n=257) were obtained to evaluate the [effect of immunoglobulins (Ig) on early bovine embryo development in vitro. Fifty-four cows superovulations were conducted in 36 cows with follicle stimulating hormone. Embryos were collected by non-surgical procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in Ham's F-10 containing 10% (6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma . globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml) bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS plus 1% 1gM. Embryos were cultured to the hatched blastocyst stage or degeneration and evaluated at 12 h intervals.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Dairy Science

Department dc:contributor.department

Dairy Science

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1985

Author dc:creator

• Sedano, Rodolfo Canseco

Chair dc:contributor.committeechair

• Gwazdauskas, Francis C.

Committee members dc:contributor.committeemember

• Vinson, William E.
• Barnes, Michael A.
• White, John M.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-03122013-040259

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/41575