{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/41128"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/41128","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Particle Manipulation Using Electric Field Gradients in Microdevices","abstract":"Electrokinetics is a family of effects that induces motion of a liquid or a particle within a liquid in response to an external electric field. Using the intrinsic electrical properties of bacteria and of breast cancer cells, electrokinetics can be used to manipulate these particles for two different types of applications: tissue engineering and breast cancer detection. The first application studied the effects of electric fields on bacteria cells as well as calcium ions to potentially create a meniscus scaffold with hydroxyapatite ends for anchoring. In response to the electric field, calcium ions were able to deposit locally and simultaneously with cellulose growth. Bacteria cells were also studied to determine their response under an AC field. At low frequencies, bacteria demonstrated controlled movement caused by electroosmosis and dielectrophoresis with a net motion caused by a dielectrophoretic force. In the second application, the separation capabilities of different stages of breast cancer cells from the same cell line were tested using contactless dielectrophoretic (cDEP) devices. The electric field gradients in cDEP devices were altered to optimize selectivity and to determine an estimated membrane capacitance for each. From the results, the membrane capacitance of the early to intermediate stages proved to be very similar; however, late stage breast cancer cells have potential in being separated from early and intermediate stages.","abstract_html":"Electrokinetics is a family of effects that induces motion of a liquid or a particle within a liquid in response to an external electric field. Using the intrinsic electrical properties of bacteria and of breast cancer cells, electrokinetics can be used to manipulate these particles for two different types of applications: tissue engineering and breast cancer detection. The first application studied the effects of electric fields on bacteria cells as well as calcium ions to potentially create a meniscus scaffold with hydroxyapatite ends for anchoring. In response to the electric field, calcium ions were able to deposit locally and simultaneously with cellulose growth. Bacteria cells were also studied to determine their response under an AC field. At low frequencies, bacteria demonstrated controlled movement caused by electroosmosis and dielectrophoresis with a net motion caused by a dielectrophoretic force. In the second application, the separation capabilities of different stages of breast cancer cells from the same cell line were tested using contactless dielectrophoretic (cDEP) devices. The electric field gradients in cDEP devices were altered to optimize selectivity and to determine an estimated membrane capacitance for each. From the results, the membrane capacitance of the early to intermediate stages proved to be very similar; however, late stage breast cancer cells have potential in being separated from early and intermediate stages.","abstract_has_math":false,"creators":["Rojas, Andrea Diane"],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Materials Science and Engineering","degree_department":"Materials Science and Engineering","school":null,"contributors":[],"advisors":[],"committee_chairs":["Davalos, Rafael V."],"committee_members":["Aning, Alexander O.","Whittington, Abby R.","Schmelz, Eva M."],"year":2012,"date_issued":"2012-02-01","date_published":"2012-02-01","updated_at":"2026-07-22T22:20:37Z","subjects":["Electrokinetics","Bacterial Cellulose","Breast Cancer","Contactless Dielectrophoresis"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-02142012-134924"],"render_values":[{"text":"etd-02142012-134924","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/41128","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Davalos, Rafael V."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Aning, Alexander O.","Whittington, Abby R.","Schmelz, Eva M."]},{"key":"dc:contributor.department","label":"Department","values":["Materials Science and Engineering"]},{"key":"dc:creator","label":"Author","values":["Rojas, Andrea Diane"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:29:41Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:29:41Z","2012-04-02"]},{"key":"dc:date.issued","label":"Date","values":["2012-02-01"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Electrokinetics","Bacterial Cellulose","Breast Cancer","Contactless Dielectrophoresis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-02142012-134924"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/41128"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Electrokinetics is a family of effects that induces motion of a liquid or a particle within a liquid in response to an external electric field. Using the intrinsic electrical properties of bacteria and of breast cancer cells, electrokinetics can be used to manipulate these particles for two different types of applications: tissue engineering and breast cancer detection. The first application studied the effects of electric fields on bacteria cells as well as calcium ions to potentially create a meniscus scaffold with hydroxyapatite ends for anchoring. In response to the electric field, calcium ions were able to deposit locally and simultaneously with cellulose growth. Bacteria cells were also studied to determine their response under an AC field. At low frequencies, bacteria demonstrated controlled movement caused by electroosmosis and dielectrophoresis with a net motion caused by a dielectrophoretic force. In the second application, the separation capabilities of different stages of breast cancer cells from the same cell line were tested using contactless dielectrophoretic (cDEP) devices. The electric field gradients in cDEP devices were altered to optimize selectivity and to determine an estimated membrane capacitance for each. From the results, the membrane capacitance of the early to intermediate stages proved to be very similar; however, late stage breast cancer cells have potential in being separated from early and intermediate stages."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Particle Manipulation Using Electric Field Gradients in Microdevices"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Davalos, Rafael V."],"dc:contributor.committeemember":["Aning, Alexander O.","Whittington, Abby R.","Schmelz, Eva M."],"dc:contributor.department":["Materials Science and Engineering"],"dc:creator":["Rojas, Andrea Diane"],"dc:date.accessioned":["2014-03-14T21:29:41Z"],"dc:date.available":["2014-03-14T21:29:41Z","2012-04-02"],"dc:date.issued":["2012-02-01"],"dc:description.abstract":["Electrokinetics is a family of effects that induces motion of a liquid or a particle within a liquid in response to an external electric field. 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The electric field gradients in cDEP devices were altered to optimize selectivity and to determine an estimated membrane capacitance for each. From the results, the membrane capacitance of the early to intermediate stages proved to be very similar; however, late stage breast cancer cells have potential in being separated from early and intermediate stages."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-02142012-134924"],"dc:identifier.uri":["http://hdl.handle.net/10919/41128"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["Electrokinetics","Bacterial Cellulose","Breast Cancer","Contactless Dielectrophoresis"],"dc:title":["Particle Manipulation Using Electric Field Gradients in Microdevices"],"dc:type":["Thesis"],"thesis:degree_discipline":["Materials Science and Engineering"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:37Z"}