{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/39955"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/39955","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12","abstract":"The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the <i>glp</i> regulon of Escherichia coli and are under negative transcriptional regulation by the <i>glpR</i>-encoded repressor. The <i>glpR</i> gene is adjacent to, and is transcribed divergently from, <i>glpD</i>. <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of <i>glpD</i> was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a <i>glpD-lacZ</i>- translational fusion.","abstract_html":"The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the &lt;i&gt;glp&lt;/i&gt; regulon of Escherichia coli and are under negative transcriptional regulation by the &lt;i&gt;glpR&lt;/i&gt;-encoded repressor. The &lt;i&gt;glpR&lt;/i&gt; gene is adjacent to, and is transcribed divergently from, &lt;i&gt;glpD&lt;/i&gt;. &lt;i&gt;GlpR&lt;/i&gt; and &lt;i&gt;glpD&lt;/i&gt; are separated by two open reading frames, designated &lt;i&gt;glpE&lt;/i&gt; and &lt;i&gt;glpG&lt;/i&gt;, encoding proteins of unknown function. The &lt;i&gt;glpD&lt;/i&gt;-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of &lt;i&gt;glpD&lt;/i&gt; was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a &lt;i&gt;glpD-lacZ&lt;/i&gt;- translational fusion.","abstract_has_math":false,"creators":["Austin, T. Denise"],"institution":"Virginia Tech","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Biochemistry and Nutrition","degree_department":"Biochemistry and Nutrition","school":null,"contributors":[],"advisors":[],"committee_chairs":["Larson, Timothy J."],"committee_members":["Dean, Dennis R.","Gregory, Eugene M.","Potts, Mokahy","Sitz, Thomas O."],"year":1991,"date_issued":"1991-05-03","date_published":"1991-05-03","updated_at":"2026-07-22T22:19:54Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10192005-113259"],"render_values":[{"text":"etd-10192005-113259","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/39955","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Larson, Timothy J."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Dean, Dennis R.","Gregory, Eugene M.","Potts, Mokahy","Sitz, Thomas O."]},{"key":"dc:contributor.department","label":"Department","values":["Biochemistry and Nutrition"]},{"key":"dc:creator","label":"Author","values":["Austin, T. Denise"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:21:29Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:21:29Z","2005-10-19"]},{"key":"dc:date.issued","label":"Date","values":["1991-05-03"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochemistry and Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph. 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The <i>glpR</i> gene is adjacent to, and is transcribed divergently from, <i>glpD</i>. <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of <i>glpD</i> was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a <i>glpD-lacZ</i>- translational fusion."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Larson, Timothy J."],"dc:contributor.committeemember":["Dean, Dennis R.","Gregory, Eugene M.","Potts, Mokahy","Sitz, Thomas O."],"dc:contributor.department":["Biochemistry and Nutrition"],"dc:creator":["Austin, T. Denise"],"dc:date.accessioned":["2014-03-14T21:21:29Z"],"dc:date.available":["2014-03-14T21:21:29Z","2005-10-19"],"dc:date.issued":["1991-05-03"],"dc:description.abstract":["The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the <i>glp</i> regulon of Escherichia coli and are under negative transcriptional regulation by the <i>glpR</i>-encoded repressor. The <i>glpR</i> gene is adjacent to, and is transcribed divergently from, <i>glpD</i>. <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide sequence of <i>glpD</i> was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide sequence across the fusion joint of a <i>glpD-lacZ</i>- translational fusion."],"dc:description.degree":["Ph. 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