{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/39911"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/39911","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicides","abstract":"The activity of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, EC 1.1.1.34) is highly expressed in 4-day-old etiolated seedlings of normal ('DeKalb XL72AA'), dwarf (<u>d₅</u>), and albino (<u>lw₃</u>) maize (<u>Zea mays</u> L.). HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m²/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from 'Dekalb XL72AA' maize seedlings.","abstract_html":"The activity of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, EC 1.1.1.34) is highly expressed in 4-day-old etiolated seedlings of normal (&#x27;DeKalb XL72AA&#x27;), dwarf (&lt;u&gt;d₅&lt;/u&gt;), and albino (&lt;u&gt;lw₃&lt;/u&gt;) maize (&lt;u&gt;Zea mays&lt;/u&gt; L.). HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m²/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from &#x27;Dekalb XL72AA&#x27; maize seedlings.","abstract_has_math":false,"creators":["Ji, Wan"],"institution":"Virginia Tech","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Plant Physiology","degree_department":"Plant Physiology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Kushad, Mosbah M."],"committee_members":["Cramer, Carole L.","Hatzios, Kriton K.","Hess, John L.","Orcutt, David M."],"year":1991,"date_issued":"1991-05-05","date_published":"1991-05-05","updated_at":"2026-07-22T22:20:09Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-10142005-135755"],"render_values":[{"text":"etd-10142005-135755","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/39911","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Kushad, Mosbah M."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Cramer, Carole L.","Hatzios, Kriton K.","Hess, John L.","Orcutt, David M."]},{"key":"dc:contributor.department","label":"Department","values":["Plant Physiology"]},{"key":"dc:creator","label":"Author","values":["Ji, Wan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T21:21:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T21:21:20Z","2005-10-14"]},{"key":"dc:date.issued","label":"Date","values":["1991-05-05"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"dc:type.dcmitype","label":"Dc Type Dcmitype","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Plant Physiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph. 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HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m²/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from 'Dekalb XL72AA' maize seedlings."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicides"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Kushad, Mosbah M."],"dc:contributor.committeemember":["Cramer, Carole L.","Hatzios, Kriton K.","Hess, John L.","Orcutt, David M."],"dc:contributor.department":["Plant Physiology"],"dc:creator":["Ji, Wan"],"dc:date.accessioned":["2014-03-14T21:21:20Z"],"dc:date.available":["2014-03-14T21:21:20Z","2005-10-14"],"dc:date.issued":["1991-05-05"],"dc:description.abstract":["The activity of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, EC 1.1.1.34) is highly expressed in 4-day-old etiolated seedlings of normal ('DeKalb XL72AA'), dwarf (<u>d₅</u>), and albino (<u>lw₃</u>) maize (<u>Zea mays</u> L.). HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m²/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from 'Dekalb XL72AA' maize seedlings."],"dc:description.degree":["Ph. D."],"dc:format.medium":["BTD"],"dc:format.mimetype":["application/pdf"],"dc:identifier.other":["etd-10142005-135755"],"dc:identifier.uri":["http://hdl.handle.net/10919/39911"],"dc:language.iso":["en"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicides"],"dc:type":["Dissertation"],"dc:type.dcmitype":["Text"],"thesis:degree_discipline":["Plant Physiology"],"thesis:degree_level":["doctoral"],"thesis:degree_name":["Ph. 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