Quantitative assessment of daily urinary conjugates in an adult male population

The effect of a self-selected and semisynthetic diet on urinary conjugates levels was determined in 18 male adults (22-40 y). Urinary conjugates were also quantified to develop an index of detoxification using a multivariate approach. The four major urinary conjugates measured were glucuronides, sulfoconjugates, mercapturates, and amino acid conjugates. Subjects consumed a self-selected diet for three days and a semisynthetic diet for seven days. Mercapturates and amino acid conjugates were most affected by dietary change, excretion levels reduced by about 50% during the semisynthetic diet period (0.27±0.11 vs 0.14±0.02 mmol/24-h; 5.99 vs 3.03 mmol/24-h, respectively). Glucuronides were the least responsive to dietary change with no significant difference between the means of the two diet periods (self-selected diet 2.93±0.77; semisynthetic 3.21±0.29 mmol/24-h). Four methods for developing 'normal' ranges were presented: mean±SD; percentiles; principal component analysis (princomp); Mahalanobis distance (distquan). The four methods were compared. In summary, conjugate excretion levels were found to be sensitive to dietary changes, with some pathways more responsive than others. Also, the princomp and distquan methods were stressed because they are a multivariate approach which combine values for all three pathways and their interaction into a single value that would then be representative of an individual's total, or overall, detoxification level relative to the others in this group.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Human Nutrition and Foods

Department dc:contributor.department

Human Nutrition and Foods

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1992

Author dc:creator

• Lugogo, Rita de Nicolo

Chair dc:contributor.committeechair

• Webb, Ryland E.

Committee members dc:contributor.committeemember

• Thye, Forrest W.
• Bevan, David R.
• Barbeau, William E.
• Webb, Kenneth E. Jr.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-06062008-171211

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/38453