{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/38443"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/38443","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Capture filtration for concentration and detection of selected microorganisms in milk","abstract":"The effectiveness of an adsorption filter in retaining bacteria present in milk was examined. Skim milk and whole milk (100ml) were separately filtered through a 47mm adsorption filter. No significant change in total solids, total fat, and solids-not-fat percentages of skim and whole milk permeates was observed after filtration. Adsorption of Pseudomonas fluorescens at target concentrations of 103 , 102 , and 101 cells/ml was determined in 100ml of dairy standard methods buffer, nutrient broth, whole milk, and skim milk. The average percentage bacterial retentions were 95 ± 5.5%, 95 ± 2.6%, 28 ± 22.1%, and 62 ± 15.5%, respectively. A treatment was developed for milk to increase the bacterial retention of ~ fluorescens after filtration. The preferred treatment for 100ml of skim milk involved the following final concentrations (v/v): 0.80% disodium ethylenediamine-tetraacetic acid, 0.02% sodium dodecyl sulfate, pH to 7.5 with 1N sodium hydroxide. The average bacterial retention of ~ fluorescens using the treatment was 91 ± 7.1%. Enumeration of bacteria adsorbed to the filter was then conducted using impedance microbiology. When milk was inoculated with ~ fluorescens at target concentrations of 103 , 102 , and 101 cells/ml, an average log bacterial increase of 1.4 ± 0.1 (25x) was obtained. This method will allow for rapid detection of microorganisms in milk by increasing microbial load in the tested sample and eliminating the need for pre-enrichment.","abstract_html":"The effectiveness of an adsorption filter in retaining bacteria present in milk was examined. Skim milk and whole milk (100ml) were separately filtered through a 47mm adsorption filter. No significant change in total solids, total fat, and solids-not-fat percentages of skim and whole milk permeates was observed after filtration. Adsorption of Pseudomonas fluorescens at target concentrations of 103 , 102 , and 101 cells/ml was determined in 100ml of dairy standard methods buffer, nutrient broth, whole milk, and skim milk. The average percentage bacterial retentions were 95 ± 5.5%, 95 ± 2.6%, 28 ± 22.1%, and 62 ± 15.5%, respectively. A treatment was developed for milk to increase the bacterial retention of ~ fluorescens after filtration. The preferred treatment for 100ml of skim milk involved the following final concentrations (v/v): 0.80% disodium ethylenediamine-tetraacetic acid, 0.02% sodium dodecyl sulfate, pH to 7.5 with 1N sodium hydroxide. The average bacterial retention of ~ fluorescens using the treatment was 91 ± 7.1%. Enumeration of bacteria adsorbed to the filter was then conducted using impedance microbiology. When milk was inoculated with ~ fluorescens at target concentrations of 103 , 102 , and 101 cells/ml, an average log bacterial increase of 1.4 ± 0.1 (25x) was obtained. This method will allow for rapid detection of microorganisms in milk by increasing microbial load in the tested sample and eliminating the need for pre-enrichment.","abstract_has_math":false,"creators":["Byrne, Robert Duane"],"institution":"Virginia Tech","degree_name":"Ph. 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III","Hackney, Cameron Raj","Jones, Gerald M."],"year":1994,"date_issued":"1994-05-04","date_published":"1994-05-04","updated_at":"2026-07-22T22:20:18Z","subjects":[],"languages":["en"],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-06062008-170931"],"render_values":[{"text":"etd-06062008-170931","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/38443","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Bishop, J. Russell"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Duncan, Susan E.","Eigel, William N. 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No significant change in total solids, total fat, and solids-not-fat percentages of skim and whole milk permeates was observed after filtration. Adsorption of Pseudomonas fluorescens at target concentrations of 103 , 102 , and 101 cells/ml was determined in 100ml of dairy standard methods buffer, nutrient broth, whole milk, and skim milk. The average percentage bacterial retentions were 95 ± 5.5%, 95 ± 2.6%, 28 ± 22.1%, and 62 ± 15.5%, respectively. A treatment was developed for milk to increase the bacterial retention of ~ fluorescens after filtration. The preferred treatment for 100ml of skim milk involved the following final concentrations (v/v): 0.80% disodium ethylenediamine-tetraacetic acid, 0.02% sodium dodecyl sulfate, pH to 7.5 with 1N sodium hydroxide. The average bacterial retention of ~ fluorescens using the treatment was 91 ± 7.1%. Enumeration of bacteria adsorbed to the filter was then conducted using impedance microbiology. When milk was inoculated with ~ fluorescens at target concentrations of 103 , 102 , and 101 cells/ml, an average log bacterial increase of 1.4 ± 0.1 (25x) was obtained. This method will allow for rapid detection of microorganisms in milk by increasing microbial load in the tested sample and eliminating the need for pre-enrichment."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Capture filtration for concentration and detection of selected microorganisms in milk"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Bishop, J. Russell"],"dc:contributor.committeemember":["Duncan, Susan E.","Eigel, William N. 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A treatment was developed for milk to increase the bacterial retention of ~ fluorescens after filtration. The preferred treatment for 100ml of skim milk involved the following final concentrations (v/v): 0.80% disodium ethylenediamine-tetraacetic acid, 0.02% sodium dodecyl sulfate, pH to 7.5 with 1N sodium hydroxide. The average bacterial retention of ~ fluorescens using the treatment was 91 ± 7.1%. Enumeration of bacteria adsorbed to the filter was then conducted using impedance microbiology. When milk was inoculated with ~ fluorescens at target concentrations of 103 , 102 , and 101 cells/ml, an average log bacterial increase of 1.4 ± 0.1 (25x) was obtained. This method will allow for rapid detection of microorganisms in milk by increasing microbial load in the tested sample and eliminating the need for pre-enrichment."],"dc:description.degree":["Ph. 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