{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/37559"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/37559","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Maize dwarf mosaic virus: purification, some physical properties, and yield of virus from several corn hybrids","abstract":"To better understand the nature of maize dwarf mosaic virus (MDMV) and its interaction with susceptible and resistant corn hybrids, the purification of MDMV and its physical properties were re-evaluated and a rapid assay procedure was developed. The virus was purified by chloroform clarification and differential and sucrose density-gradient centrifugation. Corn tissue was homogenized in 0.1 M sodium citrate plus 0.5% mercaptoethanol. The virus was resuspended after high speed centrifugation in 0.005 M sodium citrate, pH 7.0. Purified virus has an ultraviolet absorption spectrum typical for filamentous viruses having 5% RNA, shows birefringence, has an A260/280 of 1.18, and a sedimentation coefficient of 160-162 S. The virus is filamentous and appears to be a member of the potato virus Y group. A yield of 9.0-23.0 llg of virus/g fresh weigh.t of field-grown, mechanically inoculated Hy X Cl03 was determined by analytical sucrose density-gradient centrifugation. This represents a yield of purified virus of approximately 25% of that in the clarified sap. Results obtained from the density-gradient analysis were comparable to but more sensitive than those from the microprecipitin test and a systemic infectivity assay. The systemic infectivity assay on corn, analyzed by maximum likelihood and log log transformation, showed a yield of purified virus of 0.4% of that in the crude sap.","abstract_html":"To better understand the nature of maize dwarf mosaic virus (MDMV) and its interaction with susceptible and resistant corn hybrids, the purification of MDMV and its physical properties were re-evaluated and a rapid assay procedure was developed. The virus was purified by chloroform clarification and differential and sucrose density-gradient centrifugation. Corn tissue was homogenized in 0.1 M sodium citrate plus 0.5% mercaptoethanol. The virus was resuspended after high speed centrifugation in 0.005 M sodium citrate, pH 7.0. Purified virus has an ultraviolet absorption spectrum typical for filamentous viruses having 5% RNA, shows birefringence, has an A260/280 of 1.18, and a sedimentation coefficient of 160-162 S. The virus is filamentous and appears to be a member of the potato virus Y group. A yield of 9.0-23.0 llg of virus/g fresh weigh.t of field-grown, mechanically inoculated Hy X Cl03 was determined by analytical sucrose density-gradient centrifugation. This represents a yield of purified virus of approximately 25% of that in the clarified sap. Results obtained from the density-gradient analysis were comparable to but more sensitive than those from the microprecipitin test and a systemic infectivity assay. The systemic infectivity assay on corn, analyzed by maximum likelihood and log log transformation, showed a yield of purified virus of 0.4% of that in the crude sap.","abstract_has_math":false,"creators":["Jones, Ronald K."],"institution":"Virginia Tech","degree_name":"Ph. D.","degree_level":"doctoral","degree_discipline":"Plant Pathology","degree_department":"Plant Pathology","school":null,"contributors":[],"advisors":[],"committee_chairs":["Tolin, Sue A."],"committee_members":["Hale, Maynard G.","Genter, C. F.","Roane, Curtis W.","Fox, J. A.","Stipes, R. 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The systemic infectivity assay on corn, analyzed by maximum likelihood and log log transformation, showed a yield of purified virus of 0.4% of that in the crude sap."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph. D."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["BTD"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Maize dwarf mosaic virus: purification, some physical properties, and yield of virus from several corn hybrids"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Tolin, Sue A."],"dc:contributor.committeemember":["Hale, Maynard G.","Genter, C. F.","Roane, Curtis W.","Fox, J. A.","Stipes, R. 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