{"id":{"repo_id":"vt","oai_identifier":"oai:vtechworks.lib.vt.edu:10919/37017"},"canonical_url":"https://search.dev.ndltd.org/etd/vt/oai:vtechworks.lib.vt.edu:10919/37017","repository":{"repo_id":"vt","name":"Virginia Tech","base_url":"https://vtechworks.lib.vt.edu/oai/request"},"display":{"title":"Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families","abstract":"Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones.","abstract_html":"Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones.","abstract_has_math":false,"creators":["Cutright, Rebecca J."],"institution":"Virginia Tech","degree_name":"Master of Science","degree_level":"masters","degree_discipline":"Horticulture","degree_department":"Horticulture","school":null,"contributors":[],"advisors":[],"committee_chairs":["Veilleux, Richard E."],"committee_members":["Beers, Eric P.","Griffey, Carl A."],"year":1998,"date_issued":"1998-07-16","date_published":"1998-07-16","updated_at":"2026-07-22T22:20:23Z","subjects":["Potato","Anther Culture","Haploid-inducing Pollinator"],"languages":[],"rights":["In Copyright"],"rights_urls":["http://rightsstatements.org/vocab/InC/1.0/"],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-8598-184718"],"render_values":[{"text":"etd-8598-184718","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/10919/37017","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Veilleux, Richard E."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Beers, Eric P.","Griffey, Carl A."]},{"key":"dc:contributor.department","label":"Department","values":["Horticulture"]},{"key":"dc:creator","label":"Author","values":["Cutright, Rebecca J."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2014-03-14T20:52:30Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2014-03-14T20:52:30Z","2012-11-20"]},{"key":"dc:date.issued","label":"Date","values":["1998-07-16"]},{"key":"dc:publisher","label":"Institution","values":["Virginia Tech"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Horticulture"]},{"key":"thesis:degree_level","label":"Degree Level","values":["masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Virginia Polytechnic Institute and State University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Potato","Anther Culture","Haploid-inducing Pollinator"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-8598-184718"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/10919/37017"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Master of Science"]},{"key":"dc:title","label":"Title","values":["Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Veilleux, Richard E."],"dc:contributor.committeemember":["Beers, Eric P.","Griffey, Carl A."],"dc:contributor.department":["Horticulture"],"dc:creator":["Cutright, Rebecca J."],"dc:date.accessioned":["2014-03-14T20:52:30Z"],"dc:date.available":["2014-03-14T20:52:30Z","2012-11-20"],"dc:date.issued":["1998-07-16"],"dc:description.abstract":["Monopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones."],"dc:description.degree":["Master of Science"],"dc:identifier.other":["etd-8598-184718"],"dc:identifier.uri":["http://hdl.handle.net/10919/37017"],"dc:publisher":["Virginia Tech"],"dc:rights":["In Copyright"],"dc:rights.uri":["http://rightsstatements.org/vocab/InC/1.0/"],"dc:subject":["Potato","Anther Culture","Haploid-inducing Pollinator"],"dc:title":["Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families"],"dc:type":["Thesis"],"thesis:degree_discipline":["Horticulture"],"thesis:degree_level":["masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Virginia Polytechnic Institute and State University"]},"updated_at":"2026-07-22T22:20:23Z"}